Effect of the host cell line on the vaccine efficacy of an attenuated porcine reproductive and respiratory syndrome virus

Calzada-Nova, Gabriela; Husmann, Robert J.; Schnitzlein, William M.; Zuckermann, Federico A.

doi:10.1016/j.vetimm.2012.05.008

Cited by 24 publications

(14 citation statements)

References 54 publications

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“…NADC-20 is a virulent North American PRRSV strain, which was first isolated in an “atypical PRRSV abortion storm” in 2001 [ 7 ]. It has been widely used for viral challenge to evaluate the efficacy of PRRSV vaccines in the U.S [ 8 ]. Compared with the other strains of PRRSV in the U.S., NADC-20 can lead to clinical fever (≥ 40°C) and more robust immune responses after infection of pigs [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…It has been widely used for viral challenge to evaluate the efficacy of PRRSV vaccines in the U.S [ 8 ]. Compared with the other strains of PRRSV in the U.S., NADC-20 can lead to clinical fever (≥ 40°C) and more robust immune responses after infection of pigs [ 8 ]. Therefore, analysis of the host immune responses elicited by two virulent strains of PRRSV will contribute to better understanding of the pathogenesis of HP-PRRSV and facilitate more effective vaccine development.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Immune Responses in Pigs Infected with Chinese Highly Pathogenic PRRS Virus Strain HV and North American Strain NADC-20

Galliher-Beckley

Wang

et al. 2017

TOVJ

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Introduction:Chinese HP-PRRSV characterized by high morbidity and mortality of all ages of pigs emerged since 2006 in China. The immune response of HP-PRRSV was never compared with conventional low pathogenic PRRSV strain. Objective:In this study, we compared the immune responses elicited by a Chinese HP-PRRSV strain HV and a North American RRSV strain NADC20 infections. Result:Pigs infected with NADC-20 showed significantly higher Ab titers than HV-PRRSV infected pigs at 9 DPI. Infection with HV-PRRSV induced a significantly higher levels of TNF-α and IL-10 in both sera and lung tissues and higher IFN-α and IFN-γ in the serum. Flow cytometry analysis showed that HV-PRRSV infected pigs generated significantly higher frequencies of NK cells in the peripheral blood and Th/memory, CTLs, and T-reg cells in the lung as compared with NADC-20 infected pigs. Conclusion:This study demonstrates that different immunity profiles were elicited by HV-PRRSV and NADC-20, and these differences may contribute to the distinct pathogenesis of HV-PRRSV and NADC-20.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of Immune Responses in Pigs Infected with Chinese Highly Pathogenic PRRS Virus Strain HV and North American Strain NADC-20

Galliher-Beckley

Wang

et al. 2017

TOVJ

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“…However, the adaptation of ASF viruses has always resulted in genomic changes to the point of reducing the virus replication in pigs such that protection is not achieved [ 136 ]. In relation to this, five different porcine cell lines of monocyte origin have been developed so far: ZMAC, IPAM WT, IPAM-CD163, WSL, and CΔ2+ [ 137 , 138 , 139 , 140 , 141 ]. In addition, the COS-cell line has shown to be highly efficient to sustain the “in vitro” replication of ASF viruses with little apparent adaptation [ 135 , 142 , 143 ].…”

Section: Approaches Towards Asfv Vaccine Developmentmentioning

confidence: 99%

Approaches and Perspectives for Development of African Swine Fever Virus Vaccines

et al. 2017

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African swine fever (ASF) is a complex disease of swine, caused by a large DNA virus belonging to the family Asfarviridae. The disease shows variable clinical signs, with high case fatality rates, up to 100%, in the acute forms. ASF is currently present in Africa and Europe where it circulates in different scenarios causing a high socio-economic impact. In most affected regions, control has not been effective in part due to lack of a vaccine. The availability of an effective and safe ASFV vaccines would support and enforce control-eradication strategies. Therefore, work leading to the rational development of protective ASF vaccines is a high priority. Several factors have hindered vaccine development, including the complexity of the ASF virus particle and the large number of proteins encoded by its genome. Many of these virus proteins inhibit the host's immune system thus facilitating virus replication and persistence. We review previous work aimed at understanding ASFV-host interactions, including mechanisms of protective immunity, and approaches for vaccine development. These include live attenuated vaccines, and "subunit" vaccines, based on DNA, proteins, or virus vectors. In the shorter to medium term, live attenuated vaccines are the most promising and best positioned candidates. Gaps and future research directions are evaluated.

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“…The predicted linear relationships between age at time of infection and peak viremia as measured by virus isolation (dotted line) and RT ‐ PCR (solid line) are shown only for adolescent pigs. Data from adult pigs are shown on the right and suggest that there is a lower risk of PRRS virus transmission in pork harvested from adult pigs (Bloemraad et al., ; Duan et al., ; Cuartero et al., ; Greiner et al., , ,b; Martin and Steverink, ; Osorio et al., ; Shin and Molitor, ; Grebennikova et al., ; Johnson et al., ; Lohse et al., ; Truong et al., ; Wasilk et al., ; Petry et al., ; Cano et al., ; Gozio and Van Woensel, ; Lopez et al., ; Martelli et al., ; Opriessnig et al., ; Calzada‐Nova et al., , ; Loving et al., ; Mondaca‐Fernandez et al., ; Mulupuri et al., ; Costers et al., ; Klinge et al., ; Vanhee et al., ; Geldhof et al., ; Gomez‐Laguna et al., ; Karniychuk et al., ; Liu et al., ; Reiner et al., ; Dwivedi et al., ; Kim et al., ; Wang et al., ; Boddicker et al., ; Diaz et al., ; Han et al., ,b; Roca et al., ; Silva‐Campa et al., ; Wei et al., ).…”

Section: Kinetics Of Prrs Virus Infection In Pigsmentioning

confidence: 99%

Fresh Pork and Porcine Reproductive and Respiratory Syndrome Virus: Factors Related to the Risk of Disease Transmission

Hall¹,

Neumann

2013

Transbound Emerg Dis

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Porcine reproductive and respiratory syndrome virus (PRRS) is a highly infectious virus. Experimentally, the disease can be induced in naïve pigs by the oral, intranasal and intramuscular routes. Depending on the virulence of the strain of the virus and the age of the pig, peak viremia can occur within 7 days of infection, and live virus can be isolated from blood or lymph nodes for several months post-infection. Young pigs tend to develop higher titres of viremia than older pigs infected by the same route and dose with the same strain of virus. Porcine reproductive and respiratory syndrome virus survives in pork harvested from infected pigs for extended periods at temperatures of -20 or -70°C. In experimentally infected pigs, survival of PRRS virus in muscle held at 4°C has been demonstrated for at least 7 days, and infectivity of the virus in these samples was confirmed by bioassay. The optimal pH range for the survival of PRRS virus is thought to be 6.0 to 7.5. The elevated pH of non-meat tissues (generally one pH unit higher) is likely to favour extended survival of PRRS virus in pig carcasses from which all superficial and deep lymph nodes have not been removed. It is likely that exsanguinated carcasses held at 4°C retain sufficient blood or lymph tissue to contain infective doses of PRRS virus. Porcine reproductive and respiratory syndrome virus is rapidly inactivated by heat, providing a predictable method to ensure that pork tissues are free of viable virus and feeding of cooked swill or garbage should not constitute a risk to pigs. While the probability of viable PRRS virus being present in a pig carcass may be low, the risk is not zero. The importation of raw pork into countries where PRRS is not endemic represents a hazard with potentially severe economic consequences.

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Effect of the host cell line on the vaccine efficacy of an attenuated porcine reproductive and respiratory syndrome virus

Cited by 24 publications

References 54 publications

Comparison of Immune Responses in Pigs Infected with Chinese Highly Pathogenic PRRS Virus Strain HV and North American Strain NADC-20

Comparison of Immune Responses in Pigs Infected with Chinese Highly Pathogenic PRRS Virus Strain HV and North American Strain NADC-20

Approaches and Perspectives for Development of African Swine Fever Virus Vaccines

Fresh Pork and Porcine Reproductive and Respiratory Syndrome Virus: Factors Related to the Risk of Disease Transmission

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