Effect of the fat globule membrane on in vitro digestion of milk fat globules with pancreatic lipase

Ye, Aiqian; Cui, Jian; Singh, Harjinder

doi:10.1016/j.idairyj.2010.06.007

Cited by 81 publications

(60 citation statements)

References 29 publications

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“…These changes of surface charges during duodenal digestion reflected changes in surface compositions, which may be attributed to the action of enzymes and bile salts of the duodenal digestion fluid. A previous study showed that bile salts as an amphiphilic agent can absorb and/or displace the components of milk fat globule membrane . Changes in zeta potential observed in this study during duodenal digestion suggested that different digestive products were obtained in colostrum and mature milk.…”

Section: Resultssupporting

confidence: 51%

“…Then the size distribution became wider, and the shoulder peak shifted towards a medium size in the next 2 h. With a further 1 h of duodenal digestion, 66.20% of particles were returned to 10–100 μm, but smaller than that of the original particles. The increase in size may indicate that particles coalesced from protein‐like materials were formed after the digestion of milk fat globule membrane proteins (MFGMPs) …”

Section: Resultsmentioning

confidence: 99%

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Protein digestion properties of Xinong Saanen goat colostrum and mature milk usingin vitrodigestion model

et al. 2019

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BACKGROUND Xinong Saanen goat milk is a raw material for goat milk‐based infant formula production. This study aims to analyze digestion properties of Xinong Saanen goat colostrum and mature milk by simulating infant gastrointestinal digestion. Zeta potential, particles size, protein profile and peptides composition of these two kinds of milk during the digestion process were studied. RESULTS Zeta‐potential values of the digested colostrum were lower than those of mature milk through the whole digestion. Absolute zeta potential of colostrum duodenal digestion samples showed a decrease from 16.63 ± 2.08 to 11.80 ± 2.03 mV while that of mature milk decreased sharply and then increased (P < 0.05). Colostrum had a larger particle size than mature milk and both milks showed decreased particle size with increasing digestion time but an increase for the last 30 min. Colostrum showed more high molecular weight (MW) proteins which cannot be hydrolyzed completely compared with mature milk. Digested peptides (< 10 kDa) were characterized using liquid chromatography combined with tandem mass spectrometry (LC–MS/MS). The casein‐derived peptides identified in digested colostrum and mature milk accounted for 76.67% and 59.53%, respectively. β‐Casein was the most abundant in colostrum while that in mature milk was αs1‐casein. Enterotoxin‐binding glycoprotein PP20K, butyrophilin subfamily 1 member A1 (BTN1A1) and perilipin (PLIN) were only detected in digested mature milk. CONCLUSION Differences in digestion properties between goat colostrum and mature milk were mainly shown in duodenal digestion phase. Data may provide useful information about utilization of goat milk for infant formula formulation. © 2019 Society of Chemical Industry

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Section: Resultssupporting

confidence: 51%

Section: Resultsmentioning

confidence: 99%

Protein digestion properties of Xinong Saanen goat colostrum and mature milk usingin vitrodigestion model

et al. 2019

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“…It is evident that breast milk had a much longer T c compared to the infant formulas, coinciding with our in vitro observation of a lag time presented earlier. It has been well established that there is an inhibitory effect of the native milk fat globular membrane on pancreatic lipase activity [68]. No major differences were found between the studied infant formulas.…”

Section: Resultsmentioning

confidence: 78%

Biophysical Aspects of Lipid Digestion in Human Breast Milk and Similac™ Infant Formulas

et al. 2014

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Physico-chemical properties of human breast milk were compared to four Similac ™ infant formulas, and correlated with in vitro free fatty acid bioaccessibility using a simulated gastrointestinal system (TIM-1). Viscoelastic measurements, as a function of pH (pH 6.5 to 3.0) and shear rate, showed lower viscosities in breast milk compared to infant formulas. Droplet size and distribution measurements showed distinct differences between the tested formulas and breast milk. During lipid digestion, a lag period was observed for only breast milk. The rate of lipolysis was found to be higher in breast milk compared to Similac ™ formulas. The total bioaccessible free fatty acids for Advance infant formula and breast milk were not statistically different for the in vitro TIM-1 model and the shifted-logistical model using one-way ANOVA (p<0.05) with a Tukey's Multiple Comparison Test. All other infant formulas had significantly lower free fatty acid bioaccessibilities at the end of the simulated digestion. A positive correlation between rate of lipolysis and droplet surface area per gram for the Similac ™ infant formulas was found. However, breast milk did not follow that trend, suggesting the possible involvement of other factors in rate of lipolysis for breast milk.

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“…1). It has been shown in recent studies that emulsion droplets undergo flocculation, dissociation and coalescence during the digestion with pancreatic lipase under the duodenal conditions (Singh & Sarkar, 2011;Ye, Cui, & Singh, 2010). The droplet aggregation may have occurred due to bridging flocculation caused by some component in the lipase extract that adsorbed to the surfaces of more than one lipid droplet.…”

Section: Emulsions Under In Vitro Duodenal Conditionsmentioning

confidence: 99%

Comparative behavior of protein or polysaccharide stabilized emulsion under in vitro gastrointestinal conditions

et al. 2016

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Effect of the fat globule membrane on in vitro digestion of milk fat globules with pancreatic lipase

Cited by 81 publications

References 29 publications

Protein digestion properties of Xinong Saanen goat colostrum and mature milk usingin vitrodigestion model

Protein digestion properties of Xinong Saanen goat colostrum and mature milk usingin vitrodigestion model

Biophysical Aspects of Lipid Digestion in Human Breast Milk and Similac™ Infant Formulas

Comparative behavior of protein or polysaccharide stabilized emulsion under in vitro gastrointestinal conditions

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