Effect of the extract from custard apple (Annona squamosa) leaves prepared with pulsed electric field‐assisted process on the diversity of microorganisms and shelf‐life of refrigerated squid rings

Abstract

Antibacterial and antibiofilm properties of ethanolic custard apple leaf extract (CALE) prepared without and with pulsed electric field (PEF) assisted process (6 kV cm −1 , 300 pulses, 142 kJ kg −1 for 5 min) (PEF-CALE) were comparatively investigated. PEF-CALE showed higher bacterial inhibition against Bacillus subtilis, Listeria monocytogenes, Escherichia coli, and Pseudomonas aeruginosa, compared to CALE. Antibiofilm properties of both extracts towards the aforementioned bacteria were augmented with increasing concentrations (P < 0.05). The impact of PEF-CALE in extending the shelf-life of squid rings (SQR) was also investigated. Based on the microbiological limit, the shelf-life of refrigerated squid rings pretreated with 400 mg kg −1 PEF-CALE was extended by more than 6 days when compared to the control. Pseudomonas lundensis, Pseudomonas fragi, and Shewanella baltica were the most abundant spoilage bacteria found in SQR. Overall, PEF-CALE exhibited promising antimicrobial activities, which could serve as biopreservative agent for inhibiting bacterial growth in SQR during refrigerated storage.

Antioxidant and antibacterial activities of indigenous plant leaf ethanolic extracts and their use for extending the shelf‐life of Nile tilapia (Oreochromis niloticus) mince

“…Phenolic compounds have the ability to react with cell membrane by interfering with normal cell wall balance, leading to the leakage and infiltration of other compounds. The extract also affected internal organelles, which caused inhibition of growth of bacterial cells (Abuga et al ., 2020; Olatunde et al ., 2021). Since GK and RK extracts had highest TPC, they plausibly had the highest inhibition effect toward the tested bacteria than LP and SS counterparts ( P < 0.05).…”

“…Phenolic compounds are substances occurring naturally in many plants, such as vegetables, fruits, spices, herbs, and whole grains and have antioxidant and antimicrobial activities (Olatunde et al ., 2021). Tannins, flavonoids, gallic acid, catechins, etc, have been reported in plants at varying levels depending on type of plant, maturation, etc.…”

Ethanolic Cashew Leaf Extract: Antimicrobial Activity, Mode of Action, and Retardation of Spoilage Bacteria in Refrigerated Nile Tilapia Slices

“…Those specific perishable organisms (SSOs) can grow quickly and produce metabolites at low temperatures [ 34 ]. P. aeruginosa was found in tilapia sashimi [ 35 ], squid rings [ 36 ], and smoked salmon [ 37 ]. For rainbow trout fillets, Shewanella sp.…”

Extraction of natural bioactive compounds using clean label technologies and their application as muscle food preservatives

“…The antibacterial and antibiofilm properties of the ethanolic extract derived from custard apple leaf have been demonstrated. When custard apple leaf extract was combined with PEF it showed better bactericidal properties against Bacillus subtilis, Listeria monocytogenes, Escherichia coli, and Pseudomonas aeruginosa (Olatunde et al, 2021). It was recorded that the custard apple leaf (400 mg/kg) pre-treatment before PEF extended the shelf life of refrigeratedsquid rings more than 6 days (Olatunde et al, 2021).…”