Effect of the Elicitor Hyaluronic acid on Multiplication and Indirect Regeneration of Curcuma longa L. Plant

Abstract

The aim of this study was to evaluate the effect of hyaluronic acid (HA) concentrations for multiplication and indirect regeneration of Curcuma longa L. plant. Multiplied shoots of C. longa L. were used as explant materials and callus for indirect regeneration. The explants cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of HA as0.05, 0.5, 1.5, 3.0 and 15 mg l -1 for multiplication and 0.0, 0.16, 0.33, 0.66, 1.00, 2.00 and 5.00 mg l −1 for indirect regeneration. Concerning to multiplication, the highest number of shoot (3.77 shoots explant -1 ) was obtained from 3 mg l −1 HA. While, the least concentrations of HA (0.16, 0.33 and 0.66 mg l -1 ) enhanced shoot regeneration. On the other hand, the highest concentrations of HA produced higher number of roots and heaviest weight of callus under light condition.