Effect of the Culture System on Clostridium argentinense Toxigenicity in Coculture with Pseudomonas mendocina

Centorbi, H.J.; Silva, H.J.

doi:10.1006/anae.1999.0220

Cited by 2 publications

(1 citation statement)

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“…Additionally, a remarkable interaction was observed between C. argentinense G 89 and P. mendocina. In this case the production of botulinic toxin by the co-culture was 17 times higher (Centorbi and Silva 2000). Pseudomonas mendocina, originally isolated from soil in Mendoza, Argentina (Palleroni et al 1970) can produce an alginate polysaccharide (Govan et al 1981) and has potential for industrial alginate production since it has no strict fermentation requirements, no known pathogenicity and gives a good yield of product (Müller and Monte Alegre 2006).…”

Section: Introductionmentioning

confidence: 96%

Slowly utilized carbon sources enhance botulinic specific toxicity by co-culture of Clostridium argentinense with the non-pathogen Pseudomonas mendocina

Centorbi

Silva

2008

World J Microbiol Biotechnol

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The growth and botulinic toxin production of Clostridium argentinense G 89 HT in co-culture with Pseudomonas mendocina were studied using two culture systems with carbon sources of rapid and slow utilization. Growth with glucose in homogeneous co-culture presented a dual-phase progression, with the toxin produced in the slow-growing phase. The extended 50 h growth period of the second phase at low specific growth rate was attributed to the combined metabolization of glucose and a complex carbon source of the alginate type produced by P. mendocina under strongly reducing conditions. With dextrin, the co-culture grew at the lower specific growth rate (l = 0.03 h -1 ) for a period lasting 80 h. This fully enhanced the production of toxin with a specific toxicity 2.5 times higher than with glucose in a homogeneous system and 10.7 higher than that of C. argentinense G 89 HT single culture. The heterogeneous co-culture obtained with a dialysis membrane physically separating both bacteria (thereby eliminating the metabolic interaction) produced the lowest levels of growth and toxin of all the cases analyzed.

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Section: Introductionmentioning

confidence: 96%