Effect of the Copper Status of the Rat on the Copper-Molybdenum-Sulfate Interaction

Gray, Louise F.; Daniel, Louise J.

doi:10.1093/jn/84.1.31

Cited by 41 publications

(8 citation statements)

References 15 publications

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“…Many reports showed that high Mo could induce secondary low Cu or Cu deficiency in cattle, goat, horse, sheep, rat, and rabbit [3,[14][15][16][17][18][19][20][21]. But there have been no reports about the observation of apoptosis of splenocytes in secondary low Cu or Cu deficiency induced by high Mo in animals so far.…”

Section: Discussionmentioning

confidence: 99%

Increased Apoptotic Lymphocyte Population in the Spleen of Young Chickens Fed on Diets High in Molybdenum

Yang

Cui

Xiao

et al. 2010

Biol Trace Elem Res

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The experiment was conducted with the objective of examining the effects of high molybdenum (Mo) on the apoptosis of splenic lymphocytes in broilers by the methods of experimental pathology and flow cytometry (FCM). Three hundred 1-day-old avian broilers were divided into four groups of 75 each and were fed on control diet (Mo 13 mg/kg) and high Mo diets (Mo 500 mg/kg, high Mo group I; Mo 1,000 mg/kg, high Mo group II; and Mo 1,500 mg/kg, high Mo group III) for 42 days. The results showed that the relative weight of spleen was decreased, and lymphocytes were histopathologically decreased in high Mo groups II and III. Ultrastructurally, the apoptotic cells showed typical condensed nuclei with dark-round, petal-like, and horseshoe-like shapes. The mitochondria were swelled, and the endoplasmic reticulum and the Golgi apparatus were dilated in high Mo groups II and III. The statistical analyses by FCM indicated that the percentage of cellular apoptosis was higher in high Mo groups II and III than in control group. Also, the TUNEL staining was consistent with the results of FCM. It was concluded that dietary molybdenum in 1,000 and 1,500 mg/kg caused splenic lesions and lymphocyte apoptosis, which could inhibit the development of spleen and could impair immune function in broilers.

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Section: Discussionmentioning

confidence: 99%

Increased Apoptotic Lymphocyte Population in the Spleen of Young Chickens Fed on Diets High in Molybdenum

Yang

Cui

Xiao

et al. 2010

Biol Trace Elem Res

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“…1). Gray and Daniel (1964) found that normal liver Mo concentrations of 2-4 mg kg -1 could be increased to 30 mg kg -1 if the animals were fed a diet high in Mo. Puls (1980) found normal liver Mo levels to be 2.3-4.6 mg kg -1 (converted to a dry matter basis), which corresponded with the liver concentrations found at the beginning of the grazing season in 1994 and 1995, but not in 1996, when values were slightly higher.…”

Section: Livermentioning

confidence: 99%

Copper and health status of cattle grazing high-molybdenum forage from a reclaimed mine tailing site

Gardner¹,

Broersma²,

Popp³

et al. 2003

Can. J. Anim. Sci.

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. 2003. Copper and health status of cattle grazing high-molybdenum forage from a reclaimed mine tailing site. Can. J. Anim. Sci. 83: 479-485. High concentrations (21-44 mg kg -1 dry matter) of Mo have been identified in forage in several reclaimed mining areas in British Columbia. Since Mo concentrations greater than 5 mg kg -1 in forage dry matter may result in molybdenosis because of a secondary Cu deficiency in ruminants, a study was undertaken to determine if cattle can safely graze the reclaimed land. For 12-wk grazing periods in 1994, 1995 and 1996, 32 cow/calf pairs grazed high-Mo forage at a reclaimed mine tailings site located at the Highland Valley Copper mine near Logan lake, BC. Half of the animals in the trial received a Cu supplement (All-Trace copper bolus) and the other half served as a control group. There were no significant differences (P < 0.05) in weight gain, liver Mo, serum Cu and Mo, and milk Cu and Mo between the two treatment groups of cows. Liver Cu was higher for the Cu bolus group at certain time periods in 1994 and 1995, indicating that the bolus was effective at supplying Cu. At all times, the liver Cu levels for the animals remained above the recommended critical level of 25 mg kg -1 dry matter. Animals appeared healthy and no signs of Cu deficiency were observed. . Une concentration de Mo supérieure à 5 mg par kg de matière sèche pouvant entraîner la molybdénose consécutive-ment à une carence secondaire en cuivre (Cu) chez les ruminants, les auteurs ont tenté d'établir si les bovins peuvent paître sans danger sur ces terres. Trente-deux couples vaches/veaux ont brouté dans des champs à forte concentration de Mo pendant 12 semaines en 1994, 1995 et 1996, sur d'anciens sites restaurés à la mine de cuivre Highland Valley, près de Logan Lake, en Colombie-Britannique. La moitié des animaux ont reçu un supplément de Cu (cuivre pour bol alimentaire All-Trace), l'autre moitié servant de témoins. Les auteurs n'ont relevé aucune variation significative (P < 0,05) entre les deux traitements pour le gain de poids et la concentration de Mo dans le foie, de Cu et de Mo dans le sang, et de Cu et de Mo dans le lait. Le groupe qui avait reçu le Cu alimentaire présentait plus de Cu dans le foie à certains moments, en 1994 et 1995, signe que cette technique donne de bons résultats pour compenser le manque de Cu. La concentration de Cu dans le foie des animaux reste toujours au-dessus du seuil critique recommandé de 25 mg par kg de matière sèche. Les animaux semblaient tous en bonne santé et aucun symptôme de carence en cuivre n'a été observé.

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“…Although it cannot be excluded that microbial processes in the gastro-intestinal tract of rats may have some influence, in particular when sulphur compounds are involved, it was assumed that they play a minor role compared to those in the rumen; supportive evidence for this assumption is given by the finding that additional dietary Cu, Mo or S has no influence on the absorption of OSMo in sheep when this is administered via the duodenum (Mason et al 1978). Gray & Daniel (1964) suggested that the Cu status of the rat might be of importance when the influence of SO, on the Cu-Mo interaction is investigated. More evidence for a possible role of the Cu status in the Cu-Mo interaction is given by the observation that in Mo-fed rabbits the increased plasma Cu concentration can be decreased by raising the dietary Cu content while maintaining the dietary Mo supply (Gaballah, Abood, Kapsalis et al 1965).…”

mentioning

confidence: 99%

The influence of molybdenum on the copper metabolism of the rat at different Cu levels of the diet

Nederbragt

1980

Br J Nutr

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1.Male WAG/Cpb inbred rats fed on rations with approximately 1-5 rng copper/kg (deficient), 6.0 mg Cu/kg (adequate) and 25.0 mg Cu/kg (excess) were supplemented with varying amounts of molybdenum (0, 50, 150 and 500 mg/kg diet) and the effect on the Cu concentration of blood, plasma, liver and kidney, the caeruloplasmin activity of plasma and the Mo concentration of liver and kidney were studied.2. M o increased the Cu concentration of blood, plasma, liver and kidney and the Mo concentration of liver and kidney. 3. In the plasma of Mo-supplemented rats the presence of a Cu-containing fraction was demonstrated, the Cu of which did not react with dithiocarbamate and was not related to caeruloplasmin. The Cu in this fraction was not able to increase the caeruloplasmin activity in the plasma of Cu-deficient Mo-supplemented rats. The Cu concentration of the erythrocytes did not seem to have been increased by the Mo treatment.4. When compared to Cu-adequate rats the effect of Mo on the Cu distribution was reduced both by Cu deficiency and Cu excess. This decreased effect of Mo was explained by reduced uptake or retention of MO in the body as observed in the liver and kidney.In both ruminants and single-stomached animals (non-ruminants) molybdenum alone or in combination with sulphate interacts with copper metabolism. In ruminants Mo decreases the Cu concentration of blood and liver, an effect that is enhanced by SO,. In non-ruminants the Cu concentration of blood and liver is increased by Mo, an effect that is counteracted by SO,. In ruminants the Cu-Mo interaction may be explained by the dominating role of microbial processes in the rumen at the Mo levels commonly used in ruminant diets; it is suggested that the microbial-mediated formation of Cu-thiomolybdate complexes in the rumen prevents Cu absorption (Suttle, 1974; Dick et al. 1975). However, in sheep it has been observed that Mo has some effects comparable to those in non-ruminants. These effects are: an increase in the free plasma Cu concentration and the presence of a 'tightly' bound, non-caeruloplasmin Cu fraction (Smith et al. . 1975). This resemblance in influence of Mo on systemic Cu in ruminants and non-ruminants suggests that the effect of Mo feeding in ruminants may be the result of two interactive processes of which the first may be found in the rumen and the second (comparable to that in nonruminants) in the tissues. It was the purpose of this study to investigate the systemic effect of Mo on Cu metabolism in more detail, using rats as an animal model.

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Effect of the Copper Status of the Rat on the Copper-Molybdenum-Sulfate Interaction

Cited by 41 publications

References 15 publications

Increased Apoptotic Lymphocyte Population in the Spleen of Young Chickens Fed on Diets High in Molybdenum

Increased Apoptotic Lymphocyte Population in the Spleen of Young Chickens Fed on Diets High in Molybdenum

Copper and health status of cattle grazing high-molybdenum forage from a reclaimed mine tailing site

The influence of molybdenum on the copper metabolism of the rat at different Cu levels of the diet

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