Effect of the Anchoring Layer and Transport Type on the Adsorption Kinetics of Lambda Carrageenan

Michna, Aneta; Maciejewska-Prończuk, Julia; Pomorska, Agata; Wasilewska, Monika; Kilicer, Tayfun; Witt, Julia; Özcan, Özlem

doi:10.1021/acs.jpcb.1c03550

Cited by 7 publications

(6 citation statements)

References 75 publications

(202 reference statements)

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“…It was found that λ-carrageenan can form both loosely and highly packed structures depending on the anchoring layer type [ 219 ]. For example, the adsorption of λ-carrageenan on the PAMAM dendrimer layer leads to a heavier and more viscous/soft bilayer than the one built on the bPEI layer.…”

Section: Macroionsmentioning

confidence: 99%

“…For example, the adsorption of λ-carrageenan on the PAMAM dendrimer layer leads to a heavier and more viscous/soft bilayer than the one built on the bPEI layer. Moreover, the λ-carrageenan chains tend to adsorb in the “side-on” conformation for low initial bulk concentrations of the polysaccharide, whereas for high bulk concentrations, the “end-on” conformation is preferred, and the adsorbed polysaccharide chains tend to form highly hydrated quasi “polymeric brushes” [ 219 ].…”

Section: Macroionsmentioning

confidence: 99%

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Biocompatible Macroion/Growth Factor Assemblies for Medical Applications

2023

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Growth factors are a class of proteins that play a role in the proliferation (the increase in the number of cells resulting from cell division) and differentiation (when a cell undergoes changes in gene expression becoming a more specific type of cell) of cells. They can have both positive (accelerating the normal healing process) and negative effects (causing cancer) on disease progression and have potential applications in gene therapy and wound healing. However, their short half-life, low stability, and susceptibility to degradation by enzymes at body temperature make them easily degradable in vivo. To improve their effectiveness and stability, growth factors require carriers for delivery that protect them from heat, pH changes, and proteolysis. These carriers should also be able to deliver the growth factors to their intended destination. This review focuses on the current scientific literature concerning the physicochemical properties (such as biocompatibility, high affinity for binding growth factors, improved bioactivity and stability of the growth factors, protection from heat, pH changes or appropriate electric charge for growth factor attachment via electrostatic interactions) of macroions, growth factors, and macroion-growth factor assemblies, as well as their potential uses in medicine (e.g., diabetic wound healing, tissue regeneration, and cancer therapy). Specific attention is given to three types of growth factors: vascular endothelial growth factors, human fibroblast growth factors, and neurotrophins, as well as selected biocompatible synthetic macroions (obtained through standard polymerization techniques) and polysaccharides (natural macroions composed of repeating monomeric units of monosaccharides). Understanding the mechanisms by which growth factors bind to potential carriers could lead to more effective delivery methods for these proteins, which are of significant interest in the diagnosis and treatment of neurodegenerative and civilization diseases, as well as in the healing of chronic wounds.

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Section: Macroionsmentioning

confidence: 99%

Section: Macroionsmentioning

confidence: 99%

Biocompatible Macroion/Growth Factor Assemblies for Medical Applications

2023

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“…In the experiments, a homemade streaming potential (SP) cell was applied, described in detail in Refs. 30,31 . The SP cell consisted of two polished Te on blocks having two inlet and outlet compartments.…”

Section: Streaming Potential Measurements (Spm)mentioning

confidence: 99%

Neurotrophin-loaded PDADMAC/HEPARIN multilayers as an unexpected system to damage neuroblastoma cancer cells

Dąbkowska,

Stukan,

Kowalski

et al. 2023

Preprint

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Biomaterial science has contributed tremendously to developing nanoscale materials for delivering biologically active compounds, enhancing protein stability, and enabling its therapeutic use. We describe a process of formation of polyelectrolyte multilayer (PEM) prepared by sequential adsorption of positively charged polydiallyldimethylammonium chloride (PDADMAC) and negatively charged heparin sodium salt (HP), from low polyelectrolyte concentration, on a solid substrate. PEM was further applied as a platform for the adsorption of a brain-derived growth factor (BDNF), which is a protein capable of regulating neuronal cell development. The multilayers containing BDNF were thoroughly characterized by electrokinetic (streaming potential measurements, SPM) and optical (optical waveguide lightmode spectroscopy, OWLS) techniques. It was found that BDNF was successfully adsorbed on polyelectrolyte multilayers, terminated by PDADMAC and HP under physiological conditions. We further explore the effect of established PEMs in vitro on the neuroblastoma SH-SY5Y cell line. An enzyme-linked immunosorbent assay (ELISA) confirmed that BDNF was released from multilayers, and the use of the PEMs intensified its cellular uptake. PEMs significantly inhibited the proliferation of neuroblastoma cancer cells up to 72% and decreased polarization of mitochondrial membrane up to 58% (in relation to control). HPLC analysis showed that both PDADMAC and HP - terminated multilayers have antioxidative properties as they almost by half decreased lipid peroxidation in SH-SY5Y cells. Finally, enhanced formation of spheroid-like, 3D structures was observed by light microscopy. We offer a well-characterized, biodegradable PEM with antioxidant properties acting as BDNF carrier, stabilizing BDNF and making it more accessible to cells in an inhomogeneous, dynamic, and transient in vitro environment. Described multilayers can be utilized in future biomedical applications such as boosting the effect of treatment by selective anticancer as adjuvant therapy, and in biomedical research for future development of more precise neurodegenerative disease models, as they enhance cellular 3D structure formation.

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“…9 λ-carrageenan is used as a thickening agent. 10,11 The polymer matrix of carrageenan has been studied in the pharmaceutical industry as an oral extended-release tablet, as a novel extrusion that assists in the preparation of pellets, and as a stabilizer in nanoparticle systems. It has also been employed for tissue regeneration and cell transport with medicinal bio macromolecules.…”

Section: Introductionmentioning

confidence: 99%

“…Carrageenan, kappa and iota -CG, is used to form thermally reversible gels due to the presence of anhydro bridges . λ-carrageenan is used as a thickening agent. , …”

Section: Introductionmentioning

confidence: 99%

Development, Characterization, and Burn Wound-Healing Potential of Neomycin-Loaded Clay-Reinforced Nanofibers

Alotaibi,

Khan,

Ijaz

et al. 2023

ACS Omega

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Background: Skin wounds affect millions of individuals around the world, and their treatment is expensive. Objective: The purpose of this study was to make neomycinloaded CG/PVA/PAN (NCPP) nanofibers to improve wound healing. Methods: The NCPP nanofibers were characterized by using thermogravimetric analysis (TGA), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and X-ray diffraction. Drug solubility, dissolution, swelling ratio, erosion, and antibacterial studies were performed. The in vivo wound healing study of nanofibers was performed in a rabbit model and was supported by % age wound closure and histopathology. Results: The results of SEM showed some sort of agglomeration on the surface of fibers, while TGA showed 10% more stability for drug-loaded nanofibers. The drug permeation study indicated that the formulation with 15% PVA showed a controlled release profile of the drug. The NCPP nanofibers had an appreciable water retention capability. The NCPP nanofibers showed appreciable antibacterial activity against Enterococcus faecalis (Gram-positive bacteria) and Klebsiella pneumonia (Gram-negative bacteria). The wound healing study showed the better healing properties of NCPP nanofibers within 15 days. Conclusion: The findings helped us to conclude that the NCPP nanofibers were successfully fabricated and found to have a promising role in infected wound healing.

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Effect of the Anchoring Layer and Transport Type on the Adsorption Kinetics of Lambda Carrageenan

Cited by 7 publications

References 75 publications

Biocompatible Macroion/Growth Factor Assemblies for Medical Applications

Biocompatible Macroion/Growth Factor Assemblies for Medical Applications

Neurotrophin-loaded PDADMAC/HEPARIN multilayers as an unexpected system to damage neuroblastoma cancer cells

Development, Characterization, and Burn Wound-Healing Potential of Neomycin-Loaded Clay-Reinforced Nanofibers

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