Effect of the alcohol consumption on osteocyte cell processes: a molecular imaging study

Maurel, Daniel; Benaîtreau, Delphine; Jaffré, Christelle; Toumi, Hechmi; Portier, H.; Uzbekov, Rustem; Pichon, Chantal; Benhamou, Claude Laurent; Lespessailles, Éric; Pallu, Stéphane

doi:10.1111/jcmm.12113

Cited by 16 publications

(17 citation statements)

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“…However, abnormal intracellular lipid storage can cause cell and tissue damage, which is associated with several diseases [71]. In osteocytes, a strong correlation between increased lipid storage and apoptosis has been demonstrated in rat model of alcohol-induced osteopenia [72]. In the present study, the accumulation of lipid droplets in osteocytes of estrogen deficient rats may be partially associated to osteocyte apoptosis.…”

Section: Discussionsupporting

confidence: 51%

Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats

Florencio‐Silva

Sasso

Sasso-Cerri

et al. 2018

Biomedicine & Pharmacotherapy

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Estrogen maintains osteocyte viability, whereas its deficiency induces osteocyte apoptosis. As autophagy is important for osteocyte viability, we hypothesized whether the anti-apoptotic effect of estrogen is related to autophagy in osteocytes. Thirty adult female rats were sham-operated (SHAM) or ovariectomized (OVX). After three weeks, twelve rats of SHAM and OVX groups were killed before treatment (basal period), whereas the remaining rats received estrogen (OVXE) or vehicle (OVX) for 45 days. Fragments of maxilla containing alveolar process of the first molars were embedded in paraffin or Araldite. Paraffin-sections were stained with hematoxylin/eosin for histomorphometry, or subjected to the silver impregnation method for morphological analysis of osteocyte cytoplasmic processes. Autophagy was analyzed by immunohistochemical detections of beclin-1, MAP-LC3α and p62, whereas apoptosis was evaluated by immunohistochemical detections of cleaved caspase-3 and BAX, TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) method and by ultrastructural analysis. Araldite-semithin sections were subjected to the Sudan-black method for detection of lipids. OVX-basal group showed high frequency of caspase-3-, TUNEL- and p62-positive osteocytes accompanied with low frequency of beclin-1- and MAP-LC3α-positive osteocytes. At 45 days, OVXE group exhibited higher number of osteocytes, higher frequency of beclin-1- and MAP-LC3α-positive osteocytes, and lower frequency of caspase-3, BAX-, TUNEL- and p62-positive osteocytes than OVX group. Significant reduction in bone area was observed in the OVX compared to OVXE and SHAM groups. The highest frequency of Sudan-Black-positive osteocytes and osteocytes with scarce cytoplasmic processes, or showing apoptotic features were mainly observed in OVX groups. Our results indicate that estrogen deficiency decreases autophagy and increases apoptosis, whereas estrogen replacement enhances osteocyte viability by inhibiting apoptosis and maintaining autophagy in alveolar process osteocytes. These results suggest that the anti-apoptotic effect of estrogen may be, at least in part, related to autophagy regulation in osteocytes.

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Section: Discussionsupporting

confidence: 51%

Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats

Florencio‐Silva

Sasso

Sasso-Cerri

et al. 2018

Biomedicine & Pharmacotherapy

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“…5A ). Defected region were immunostained for CD44, a specific marker of MSCs, to detect endogenous MSCs in newly mineralized bone tissue ( Maurel et al, 2014 ; Osugi et al, 2012 ). The arrows in the figure indicate CD44 expression, which was identified by red staining.…”

Section: Resultsmentioning

confidence: 99%

Enhanced Healing of Rat Calvarial Bone Defects with Hypoxic Conditioned Medium from Mesenchymal Stem Cells through Increased Endogenous Stem Cell Migration via Regulation of ICAM-1 Targeted-microRNA-221

Chang¹,

Kim²,

Park³

et al. 2015

Molecules and Cells

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The use of conditioned medium from mesenchymal stem cells may be a feasible approach for regeneration of bone defects through secretion of various components of mesenchymal stem cells such as cytokines, chemokines, and growth factors. Mesenchymal stem cells secrete and accumulate multiple factors in conditioned medium under specific physiological conditions. In this study, we investigated whether the conditioned medium collected under hypoxic condition could effectively influence bone regeneration through enhanced migration and adhesion of endogenous mesenchymal stem cells. Cell migration and adhesion abilities were increased through overexpression of intercellular adhesion molecule-1 in hypoxic conditioned medium treated group. Intercellular adhesion molecule-1 was upregulated by microRNA-221 in mesenchymal stem cells because microRNAs are key regulators of various biological functions via gene expression. To investigate the effects in vivo, evaluation of bone regeneration by computed tomography and histological assays revealed that osteogenesis was enhanced in the hypoxic conditioned medium group relative to the other groups. These results suggest that behavioral changes of endogenous mesenchymal stem cells through microRNA-221 targeted-intercellular adhesion molecule-1 expression under hypoxic conditions may be a potential treatment for patients with bone defects.

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“…Globally, it is one of the major causes of osteoporosis in men (Giusti & Bianchi, ; Kim et al, ; Misiorowski, ). Higher use has been shown to decrease BMD (Alvisa‐Negrín et al, ; Lairinen, Lamberg‐Allardt, Tunninen, Harkonen, & Valimaki, ; Malik et al, ; Maurel, Boisseau, Benhamou, & Jaffre, ; Paccou et al, ; Ulhøi, Meldgaard, Steiniche, Odgaard, & Vesterby, ), and increase the number of empty osteocyte lacunae (Maurel et al, ). With continued high consumption, men may be more susceptible to BMD decrements than their female counterparts (Kanis et al, ; Malik et al, ).…”

Section: Discussionmentioning

confidence: 99%

Use of backscattered scanning electron microscopy to quantify the bone tissues of mid‐thoracic human ribs

Beresheim

Pfeiffer

Grynpas

et al. 2019

American J Phys Anthropol

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Objectives: Novel information on apartheid health conditions may be obtained through the study of recent skeletal collections. Using a backscattered scanning electron microscopy (BSE-SEM) approach, this study aims to produce bone quality and tissue mineralization data for an understudied South African population from the Western Cape province.Methods: Using BSE-SEM imaging, cortical porosity (Ct.Po), osteocyte lacunar density (Ot.Lc. Dn), and the degree of tissue mineralization were quantified in mid-thoracic ribs from the Kirsten Skeletal Collection. Individuals ( n female = 75, n male = 68, and mean age = 46.3 years)were predominantly from the South Africa Colored (SAC) population group ( n SAC = 103, 72%).Full cross-sectional images of each rib were manually stitched together in Adobe Photoshop.Photomontages were imported into MATALB (Mathworks, Natick, MA) for image processing and analysis. Age-related changes in histomorphometric parameters and sex differences were examined using correlation analysis, as well as linear and nonlinear regressions.Results: Young adult men have significantly less mineralized bone and fewer osteocyte lacunae, compared to women. Only men demonstrate a significant negative relationship between Ot.Lc.Dn and age. Average tissue mineralization decreases with age in women, while Ct.Po increases.Pore area (Po.Ar) does not vary with age, but pore density (Po.Dn) is highest in the perimenopause, when accelerated rates of bone turnover are first anticipated. Ct.Po is highest in the years following the predicted age of menopause, but levels off in the final decades of life.Conclusions: Men and women display disparate patterns of bone aging. Systemic disenfranchisement of non-white population groups affected bone health in South Africa, and may continue to do so today. Indicators of poor bone quality are evident in the full study sample, indicating that osteoporosis and fracture risk are not just of concern to the aged white female population. K E Y W O R D Sbone histomorphometry, BSE-SEM, osteoporosis, South African apartheid

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Effect of the alcohol consumption on osteocyte cell processes: a molecular imaging study

Cited by 16 publications

References 44 publications

Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats

Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats

Enhanced Healing of Rat Calvarial Bone Defects with Hypoxic Conditioned Medium from Mesenchymal Stem Cells through Increased Endogenous Stem Cell Migration via Regulation of ICAM-1 Targeted-microRNA-221

Use of backscattered scanning electron microscopy to quantify the bone tissues of mid‐thoracic human ribs

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