Effect of the Aerobic Denitrifying Bacterium Pseudomonas furukawaii ZS1 on Microbiota Compositions in Grass Carp Culture Water

Abstract

Background: Although functional bacteria are widely used in aquaculture water treatment, whether they affect the indigenous microbiota and whether the impact is persistent remain unclear. Therefore, we aimed to explore the denitrification effect of Pseudomonas furukawaii ZS1 isolated from a grass carp culture pond in nitrogen-rich aquaculture water, and determine whether its effect on the microbiota structure of the aquaculture water was persistent. Methods: Three each of treatment and control groups were set up, and P. furukawaii ZS1 was added to the treatment group. The concentrations of ammonia nitrogen, nitrite, and nitrate, and the pH of each sample were measured for eight consecutive days. Changes of microbiota composition in the water were analysed via high-throughput sequencing. Results: Ammonia, nitrite, and nitrate concentrations were substantially lower in the treatment group than in the control group. There were significant differences in the microbiota structure between treatment and control groups, especially on days 2–7 after adding P. furukawaii ZS1. Furthermore, significantly enriched bacterial genera in the treatment group were initially higher in number than inhibited genera, but subsequently reverted to being lower in number. Conclusions: These results provide theoretical guidance for the effective use of P. furukawaii ZS1 to control aquaculture water.

Berberine Relieves Metabolic Syndrome in Mice by Inhibiting Liver Inflammation Caused by a High-Fat Diet and Potential Association With Gut Microbiota

“…Although high-throughput sequencing of 16S rRNA gene has been widely used to analyze microbiota composition in various environments ( Wu et al, 2017 ; Gong et al, 2021 ), it is difficult to distinguish microorganisms into species or strain level, which hinders a more detailed analysis of the function of gut microbes at species or strain level. Additionally, it is important to further exploit the gut microbiota-derived metabolites of berberine through metabonomics analysis.…”

Method for screening water physicochemical parameters to calculate water quality index based on these parameters’ correlation with water microbiota

“…If the WQI is concerned about its indicative effect on aquatic vertebrates, it is recommended to use aquatic vertebrate communities as biological community information. Another issue should be clarified is that we had adopted the DNA high-throughput sequencing technology of 16 S rRNA gene to analyze the bacterioplankton community composition in this study, mainly because this method is widely used to analyze the composition of bacterioplankton community (such as [ 1 , [42] , [43] , [44] , [45] ]. However, this does not mean that other analysis methods of plankton community, such as morphological identification [ 20 , 46 , 47 ], are not applicable to the calculation of WQI b and WQI minb that described in this study.…”

Probiotics Have the Potential to Significantly Mitigate the Environmental Impact of Freshwater Fish Farms

“…For early life stages of cultivated fish, an appropriate administration option may be to add probiotics directly to water containing animal larva and combine this mixture with the use of enriched live feed [78][79][80]. In addition to the beneficial digestive and health effects of probiotics as feed supplements [81,82], adding probiotics to fishpond water can also be beneficial, and these benefits may include improvements in water quality, which is a critical parameter for growth, feed conversion, and farmed fish welfare [83][84][85][86]. Fish excrete NH 4 -N and N compounds in fishponds, and denitrification bacteria can convert these compounds to nitrites and nitrates.…”

“…Probiotic microorganisms have been shown to improve the quality of water in a variety of ways. Heterotrophic bacteria that require both organic and inorganic sources of carbon for growth play a critical role in the breakdown of organic matter and the generation of particulate food items from dissolved organics [83]. Some evidence can be found that suggests that probiotics in freshwater aquaculture ponds or tanks, including recirculated freshwater aquaculture systems, have the potential to reduce toxic levels of NH 3 and P [6][7][8].…”