Effect of the additives polyethylenimine and glutaraldehyde on the immobilization of Erwinia sp. D12 cells in calcium alginate for isomaltulose production

Kawaguti, Haroldo Yukio; Buzzato, Michele Ferrari; Orsi, Daniela Castilho; Suzuki, Gaby Tiemi; Sato, Hélia Harumi

doi:10.1016/j.procbio.2006.05.003

Cited by 29 publications

(29 citation statements)

References 14 publications

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“…D12 [22][23][24] , Erwinia rhapontici 10,25 , Serratia plymuthica 3,26,27 , Klebsiella planticola 9 e Klebsiella sp. 28,29 .…”

Section: Conversão De Sacarose Em Isomaltulose Por Via Microbianamentioning

confidence: 99%

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Produção de isomaltulose, um substituto da sacarose, utilizando glicosiltransferase microbiana

Kawaguti¹,

Sato²

2008

Quím. Nova

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“…D12 [22][23][24] , Erwinia rhapontici 10,25 , Serratia plymuthica 3,26,27 , Klebsiella planticola 9 e Klebsiella sp. 28,29 .…”

Section: Conversão De Sacarose Em Isomaltulose Por Via Microbianamentioning

confidence: 99%

“…Ahn et al 36 23,24 estudaram a influência da concentração de alginato de sódio, da suspensão de células e aplicação de aditivos glutaraldeído e polietilenoimina na imobilização de Erwinia sp. D12 em alginato de cálcio.…”

Section: Figura 3 Formação Do Gel De Alginato De Cálcio: (A) Homopolunclassified

Produção de isomaltulose, um substituto da sacarose, utilizando glicosiltransferase microbiana

Kawaguti¹,

Sato²

2008

Quím. Nova

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“…It is suggested that, the strong attachment of cells to the non-treated membrane resulted in the highest CGTase excretion. Kawagutiet al 5 reported that the production of isomaltulose from immobilized Erwinia sp. D12 increased when calcium alginate beads treated with 0.06% of glutaraldehyde was used, which was contradicted to the finding of this study.…”

Section: Resultsmentioning

confidence: 99%

“…Cross linking of immobilization support with chemicals is a common approach in order to strengthen the attachment of microbial cells to the solid support 4 and to improve both mechanical and chemical stability of the support, resulting in better retention and higher viability of cell, thus improve the product formation 5 . Generally, cross linking of immobilization support with chemicals involve two steps, first activation of the support and secondly binding the cell to the activated support.…”

Section: Introductionmentioning

confidence: 99%

Effect of the cross linkers’ concentration on the immobilization of recombinant Escherichia coli cells on hollow fiber membrane for excretion of cyclodextrin glucanotransferase (CGTase)

CheMan

Illias

Sulaiman

et al. 2017

Chem. Eng. Res. Bull.

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Abstract:The excretion of recombinant proteins into the culture medium is a preferred approach over cytoplasmic expression because of the high specific activity and ease of purification. However, cell lysis is one of a major problem during the excretion of recombinant protein due to the pressure build up in the periplasmic space through overproduction of the expressed recombinant protein. Cell immobilization is a promising solution for the improvement of protein excretion with reduction in cell lysis. In this study, the effect of cross linkers concentrations on the process of cell immobilization for the cyclodextringlucanotransferase (CGTase) excretion and cell viability were investigated. The hollow fiber membrane was treated using different concentrations (0.3, 0.67 and 1%) of cross linkers namely glutaraldehyde (GA), poly-L-lysine (PLL) and polyethylenimine (PEI). The optimum concentration of cross linkers was found to be 0.3% with the high excretion of CGTase. Interestingly, the immobilized cell on the non-treated hollow fiber membrane showed up to 15% increment of CGTase excretion with 55% reduction of cell lysis, as compared to the immobilized cells on the treated hollow fiber membrane. It could be concluded that, the low concentration of cross linkers exhibited the highest CGTase excretion. Moreover, the non-treated hollow fiber membrane is a promising approach for attachment of cells to the membrane without requiring treatment with any chemicals for the production of recombinant enzyme.

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“…Although few studies have been conducted specifically applying them to riboflavin production (20,21,22,28), factorial design and surface response methodology as an optimization strategy is a common practice in biotechnology with many research reports of it (1,5,10,13,15,23,27). …”

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confidence: 99%

Medium composition influence on Biotin and Riboflavin production by newly isolated Candida sp

Suzuki¹,

Macedo²,

Macedo³

2011

Braz. J. Microbiol.

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Complex B vitamins as Biotin and Riboflavin are required by living organisms, not only for growth but also for metabolite production, and the feed market classifies them as growth promoters. Since Brazil will soon be one of the world's biggest animal protein producers, feed production is a large consumer of vitamins and micronutrients. The industry requires 10 mg riboflavin/0.2 mg biotin per kilogram of feed; a ratio of 40 ~ 50:1. Although few studies have been conducted specifically on riboflavin production using factorial design and surface response method as an optimization strategy, it is a common practice in biotechnology with many research reports available. However, there are no reports on the use of statistical design for biotin production. This study set out to evaluate medium composition influence on biotin and riboflavin production using a statistical design. There are no studies relating biotin and riboflavin production by Candida sp LEB 130. In this preliminary study to improve the simultaneous production of biotin and riboflavin, the maximum riboflavin/biotin ratio of 8.3 µg/mL was achieved with medium component concentrations of: sucrose 30 g/L, KH 2 PO 4 2 g/L, MgSO 4 1 g/L and ZnSO 4 0.5mL/L.

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Effect of the additives polyethylenimine and glutaraldehyde on the immobilization of Erwinia sp. D12 cells in calcium alginate for isomaltulose production

Cited by 29 publications

References 14 publications

Produção de isomaltulose, um substituto da sacarose, utilizando glicosiltransferase microbiana

Produção de isomaltulose, um substituto da sacarose, utilizando glicosiltransferase microbiana

Effect of the cross linkers’ concentration on the immobilization of recombinant Escherichia coli cells on hollow fiber membrane for excretion of cyclodextrin glucanotransferase (CGTase)

Medium composition influence on Biotin and Riboflavin production by newly isolated Candida sp

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