Effect of the addition of two superoxide dismutase analogues (Tempo and Tempol) to alpaca semen extender for cryopreservation

Santiani, Alexei; Evangelista‐Vargas, Shirley; Valdivia, Martha; González, Jorge Hernández; Gutiérrez, Raúl Sánchez

doi:10.1016/j.theriogenology.2012.12.012

Cited by 30 publications

(23 citation statements)

References 30 publications

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“…With regard to the samples equilibrated at 5 °C, our results with glycerol are similar to those of most studies found in the literature, where it has been reported that the use of 6–8% glycerol does not promote motility despite using different extenders (llama: von Baer & Helleman, ; Aller et al ., ; alpaca: Vaughan et al ., ; Santiani et al ., ; Santiani Acosta et al ., ). On the surface, the only report that differs is that of Bravo et al .…”

Section: Discussionmentioning

confidence: 97%

“…With regard to sperm membrane integrity, all freeze–thawing protocols used in this study retained between 46 and 60% of sperm viability in the thawed samples. It is generally accepted that a substantial number (50%) of spermatozoa are damaged during cryopreservation (Watson, ), and this decrease in viability in cryopreserved semen has been observed in various species (horse: Neild et al ., ; boar: Fraser & Strzeżek, ; dog: Kim et al ., ; human: Hammadeh et al ., ) and also in SAC (llama: Aller et al ., ; alpaca: Santiani et al ., ; Santiani Acosta et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…In SAC, there is only one report in alpacas that evaluates sperm DNA fragmentation using TUNEL in frozen semen (Santiani Acosta et al ., ). They cryopreserved ejaculates with ethylene glycol and superoxide dismutase analogues, and their results showed a DNA fragmentation index that varied between 16.7 and 38.8%.…”

Section: Discussionmentioning

confidence: 99%

“…With regard to sperm membrane function, no differences were observed between any of the freeze–thawing protocols. There is only one other report in the literature (Santiani Acosta et al ., ) that evaluates this parameter in frozen–thawed SAC spermatozoa, and similar to our observations, these authors reported a decreased membrane function after cryopreservation. With regard to sperm membrane integrity, all freeze–thawing protocols used in this study retained between 46 and 60% of sperm viability in the thawed samples.…”

Section: Discussionmentioning

confidence: 99%

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Effect of cryoprotectant and equilibration temperature on cryopreservation ofLama glamaspermatozoa

et al. 2014

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The aim of this study was to determine the effect of two equilibration temperatures (5 °C and room temperature) and two cryoprotectants (glycerol and dimethylformamide, both at 7%) on llama sperm cryopreservation. Llama ejaculates were divided into four aliquots. A lactose-EDTA-egg yolk (LEEY) extender with either 7% glycerol (LEEY-G) or 7% dimethylformamide (LEEY-DMF) was added to two of the aliquots, which were equilibrated for 20 min at room temperature and subsequently frozen. The other two aliquots were extended in LEEY, cooled to 5 °C, then LEEY-G or LEEY-DMF was added, equilibrated for 20 min at 5 °C and frozen. No significant differences (P > 0.05) were observed in membrane function and chromatin condensation between any of the freeze-thawing protocols. Post-thaw motility was greater (P < 0.05) in LEEY-DMF than LEEY-G. DNA fragmentation was not different between raw and frozen semen with LEEY-DMF but was high in all samples with glycerol. Our results indicate that 7% glycerol would be detrimental for llama spermatozoa, but further studies are needed to evaluate effectiveness if used at lower concentrations. Dimethylformamide preserved motility and DNA integrity of frozen-thawed llama spermatozoa and could be used to replace glycerol at the concentrations used in this study.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of cryoprotectant and equilibration temperature on cryopreservation ofLama glamaspermatozoa

et al. 2014

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“…To improve the motility of sperm that has been frozen and then thawed, several supplements can be added to the freezing solution, including chemicals and proteins [9, 27, 31, 34]. It is known that freezing of sperm induces a lipid phase transition in the plasma membrane resulting in cholesterol depletion, which causes membrane destabilization [4].…”

Section: Introductionmentioning

confidence: 99%

Effects of Cholesterol-Loaded Cyclodextrins on the Rate and the Quality of Motility in Frozen and Thawed Rabbit Sperm

et al. 2014

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The motility of sperm after freezing and thawing is critical for effective cryopreservation. It is known that supplementation with cholesterol-loaded cyclodextrin (CLC) improves cryosurvival of sperm in various animals. To clarify the effects of supplementation with CLC on rabbit sperm motility after freezing and thawing, rabbit sperm motility was analyzed using a computer-assisted sperm analysis system. Sperm motility with CLC supplementation was 29.4 ± 9.6% (mean ± SD), which was significantly higher than that of controls (20.8 ± 7.1%, P<0.05). The curvilinear velocity of sperm with CLC exceeded that of controls, whereas the values for linearity and wobble were significantly lower in sperm with CLC compared with controls. After artificial insemination, 44.3% of recovered ova were fertilized in the CLC-supplemented group, which was higher than the percentage in the control group (36.4%). The results indicate that supplementation with CLC improves the rate and quality of motility in rabbit sperm after freezing and thawing, and would be advantageous for successful cryopreservation.

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Effect of the addition of six antioxidants on sperm motility, membrane integrity and mitochondrial function in red seabream (Pagrus major) sperm cryopreservation

Liu

Wang

Zhang

et al. 2014

Fish Physiol Biochem

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The present study was to evaluate the effects of six antioxidants on frozen-thawed sperm motility, viability, membrane integrity and mitochondrial function in red seabream (Pagrus major) by computer-assisted sperm analysis system and flow cytometry, respectively. All the parameters tested in this study were determined using one-way ANOVA and identified using the SNK test (P < 0.05). The results demonstrated that on the first day, the highest motility and longevity occurred in 100 mM trehalose (78.34 ± 3.41%, 29 ± 4.00 days) and 50 mM taurine (77.46 ± 1.54%, 29.33 ± 4.04 days), followed by 25 mM vitamin C (79.03 ± 5.37 %, 17 ± 1.00 days), 25 mM vitamin E (69.64 ± 1.64%, 27.67 ± 1.53 days) and 25 mM vitamin A (78.89 ± 2.81%, 9.33 ± 1.53 days), which were all higher than frozen-thawed sperm without antioxidant (control) (66.80 ± 5.55, 5.67 ± 1.15 days). Especially, the percentages of class A sperm with the addition of 100 mM trehalose (40.39 ± 5.20%) and 50 mM taurine (37.78 ± 3.22%) were significantly improved compared to the control (19.63 ± 5.44%). The viability of all groups on the third and sixth day showed a similar trend. Moreover, during the 4 °C storage process, the decrease of frozen-thawed sperm motility was closely associated with the decrease in membrane integrity and mitochondrial function. In conclusion, the present study indicated that antioxidant (100 mM trehalose and 50 mM taurine) provided the most pronounced protective effect in improving frozen-thawed quality of red seabream sperm. The addition of antioxidant may be capable of scavenging the ROS generated during the cryopreservation process and 4 °C storage.

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Effect of the addition of two superoxide dismutase analogues (Tempo and Tempol) to alpaca semen extender for cryopreservation

Cited by 30 publications

References 30 publications

Effect of cryoprotectant and equilibration temperature on cryopreservation ofLama glamaspermatozoa

Effect of cryoprotectant and equilibration temperature on cryopreservation ofLama glamaspermatozoa

Effects of Cholesterol-Loaded Cyclodextrins on the Rate and the Quality of Motility in Frozen and Thawed Rabbit Sperm

Effect of the addition of six antioxidants on sperm motility, membrane integrity and mitochondrial function in red seabream (Pagrus major) sperm cryopreservation

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