Effect of the addition of fly ash on the specific methane production and microbial communities in the anaerobic digestion of real winery wastewater

Abstract

BACKGROUND Fly ash is an important source of trace elements that can act as cofactors of enzyme systems and improve anaerobic digestion processes. However, its effect on anaerobic digestion using real wastewater has not yet been studied. In the present work, the effect of fly ash on the anaerobic digestion of real winery wastewaters was studied, by means of specific methanogenic activity assays and with a special focus on its effect on the specific methane production and the microbial communities present. Five fly ash concentration (25, 50, 75, 100, and 150 mg L−1) were supplemented under mesophilic conditions. RESULTS The results showed that 25 mg L−1 of fly ash improved the specific methane production by 18% compared to the control assay; meanwhile, a concentration of 100 mg L−1 of fly ash generated inhibition of the methanogenesis and a 20% specific methane production decrease with respect to the control experiment. The microbial community analysis verified that fly ash supplementation affected the bacterial communities, with a significant effect on acetogenic bacteria, such as Cloacimonetes and Firmicutes. CONCLUSION The addition of adequate concentrations of fly ash to the process can raise the relative abundance of some microorganisms and, consequently, could increase the specific methane production in the anaerobic digestion of winery wastewater. © 2021 Society of Chemical Industry (SCI).

Process Optimization and Biomethane Recovery from Anaerobic Digestion of Agro-Industry Wastes

“…However, in this study, the FA (100 ppm) amended stage 2 anaerobic process with 70% GM and 30% SG yielded lower biomethane (Figure 2f) than the anaerobic medium adapted from Moody et al [58]. Lauzurique et al [59] stated that 25 ppm FA enhanced methane production while 100 ppm inhibited it by 20%. It may be due to the imbalance of trace elements or calcium (Figure 3) by adding FA [60].…”

Iron/carbon conductive materials for enhancing anaerobic treatment of synthetic acrylamide wastewater

“…TSS and VSS were analyzed in accordance with Standard Methods for the Examination of Water and Wastewater 25 . COD was determined by rapid digestion spectrophotometry 26 (HACH‐DR900, Chongqing Instrument Co. Ltd, China).…”

“…Analytical methods TSS and VSS were analyzed in accordance with Standard Methods for the Examination of Water and Wastewater. 25 COD was determined by rapid digestion spectrophotometry 26 (HACH-DR900, Chongqing Instrument Co. Ltd, China). The content of ammonia nitrogen was measured using Nessler's reagent spectrophotometry (UV-visible spectrophotometer, EVOLUTION201, Shanghai Science & Technology, China).…”

Anodic Oxidation of Industrial Winery Wastewater Using Different Anodes

“…The ClO 3 − ion can be formed by the chemical reaction between the ClO − ion and the hypochlorous acid (HClO) (reaction ( 9)) or by direct oxidation of the ClO − ion on the electrode without the action of the hydroxyl radicals ( • OH) (reaction (10)).…”

“…In general, this industrial effluent has a high organic load that may lead to contamination if not treated correctly [6]. In addition, winery wastewater has a high concentration of suspended solids, low content of nutrients, and recalcitrant high molecular weight compounds such as polyphenols, fertilizers, and pesticides [7][8][9][10]. These compounds are not easily removed by the biological processes normally used to treat this type of wastewater, which makes it difficult to achieve a high removal of organic matter [11,12].…”