Effect of tetrandrine on the expressions of hypoxia-inducible factor-1 and vascular endothelial growth factor in neovascularized cornea

Abstract

This study investigated the effect of tetrandrine on the expressions of hypoxia inducible factor1 (HIF1 ) and vascular endothelial growth factor (VEGF) in neovascularized cornea of rats following alkaline burn. A rat model corneal neovascularization (CNV) was established by alkaline burn. The expressions of HIF1 and VEGF before and after tetrandrine treatment were detected by immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR) and slit-lamp biomicroscopy and photography were done to observe the neovascularization. Result showed the expressions of HIF1 and VEGF in the normal cornea were very weak or even absent. Following alkaline burn, the expressions of HIF1 and VEGF were markedly increased and mainly located in the neovascularized area and in the epithelial layer. After treatment with Tetrandrine, the density of neovascularization was significantly decreased, accompanied by remarkably decreased expressions of HIF1 and VEGF in the neovascularized corneal stroma. It is concluded that tetrandrine can effectively inhibit the neovascularization following alkaline burn in rats and reduce the expressions of HIF1 and VEGF in neovascularized cornea of rats following alkaline burn.

Chemical composition of n-butanol extract of Potentilla anserina L. and its protective effect of EAhy926 endothelial cells under hypoxia

“…Hypoxia injury to the endothelial cells is known to change the redox state of cell and result in pathological changes in the tissue (Wang et al, 2011;Castillo et al, 2002;Ma et al, 2011). Previous studies reported that hypoxiainduced apoptosis and necrosis of endothelial cells was related to the formation of free radicals (Sun et al, 2011;Mattson et al, 1995). In this study, we investigated the protective effects of n-butanol extract of P. anserina in the endothelial cells during the hypoxia compared with composite salviae extract (as positive control) for the first time.…”

“…Recently, a study showed that the effects of a group of 20 structurally related flavonoids, including flavones, flavonols and isoflavones, on the production of vascular endothelial growth factor (VEGF) induced by hypoxia in NCI-H157 cells were analyzed. VEGF is the main regulator of physiological and pathological angiogenesis and is highly stimulated by hypoxia-inducible factor 1 (HIF-1) (Sun et al, 2011). Structure-activity relationships demonstrated that flavones derivatives were the most active compounds and that hydroxylation of the A ring at positions 5 and 7 and of the B ring at position 4 were important for this activity (Elena et al, 2010).…”