Effect of Tetrafluoromethane Plasma Treatment of PMMA on MCF-7 Cell Proliferation

Abstract

Polymethyl methacrylate (PMMA) was surface modified by tetrafluoromethane (CF 4 ) plasma and its effect on the human breast cancer cells (MCF-7) adhesion and proliferation were investigated. The promotion of MCF-7 cell growth was positively and quantitatively related to the CF 4 plasma modification as revealed by different characterizations. From ESCA analysis, it is revealed that CF 4 plasma treatment chemically alters the capability of PMMA to adsorb proteins from cell culture medium, which is about four times comparing to that of pristine PMMA. In addition to chemical alteration, CF 4 plasma also concurrently delivered physical modification by doubling the surface roughness, as observed by AFM analysis.

Surface Roughening of Poly (methyl-methacrylate) Membranes by SF6-Glow-Discharge Plasma Etching

“…The solution was then pursed into flat-bottomed petrie-dish floated on mercury to ensure the uniformity in the membrane thickness. The solvent was allowed to evaporate slowly over a period of (12)(13)(14) hours in the dry atmosphere. The films so obtained were peeled off and dried in vacuum at 50 °C, for 2 hours in order to ensure the removal of the solvent [20].The schematic diagram of set up for low-pressure, glow-discharge plasma treatment on polymer films is shown in Fig.…”

“…Poly (methylmethacrylate) PMMA has been widely used in biomedical and optical applications for its advantages of chemically inert, transparent, excellent mechanical properties, low cost, and ease of fabrication. It has been widely used in applications such as tissue engineering [13]. PMMA is a hard, rigid, and transparent polymer with a glass transition temperature of (125)°C.…”