Effect of tetracycline on osteogenesis <i>in vitro</i>

Saxén, Lauri

doi:10.1002/jez.1401620305

Cited by 64 publications

(14 citation statements)

References 42 publications

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“…d) GTC-Iabelling represents a modern, commonly accepted labelling method. GTC has been found to have an injurious effect on bone during the embryonal stage, when given over a long period (Saxen 1966), but it is not known to have any effect on the longitudinal growth of postnatal bone when given for a short time, hence no sources of error concerning this part of the investigation should be expected. Kaitila (1971) draws the same conclusion in his work.…”

Section: Discussion Of the Present Methodsmentioning

confidence: 99%

Growth and Remodelling of the Ribs in Normal and Scoliotic Pigs

Snellman

1973

Acta Orthopaedica Scandinavica

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Section: Discussion Of the Present Methodsmentioning

confidence: 99%

Growth and Remodelling of the Ribs in Normal and Scoliotic Pigs

Snellman

1973

Acta Orthopaedica Scandinavica

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“…This is the case when tetracycline antibiotics are injected in vivo [4][5][6][7]. It has been found, however, that tetracyclines inhibit bone matrix synthesis and mineralization in vitro [8][9][10][11][12]. Whether these antibiotics will affect bone formation in vivo at the range of the size of doses normally used for labeling of bone is not known.…”

Section: Introductionmentioning

confidence: 97%

Tetracycline labeling of bone in vivo

Tam

Anderson

1980

Calcif Tissue Int

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We have analyzed various aspects of tetracycline labeling technique for the measurement of bone apposition rate in vivo. Our efforts were restricted to those aspects that are frequently questioned when data obtained using this technique are interpreted as representing the rate of bone apposition. Rat bone was labeled in vivo by sequential injections of oxytetracycline at a dose range of 3 to 24 mg/kg body weight and at intervals ranging from 24 to 72 h. The bone apposition rate was calculated by measuring the distance from the first dose of label to the subsequent ones. As these distances are by far too small to be determined accurately by any available micrometer eyepiece, we have used a scanning microscope photometer which allows measurements on slow-forming sites that otherwise would have been considered nongrowing sites. Using these techniques, we have demonstrated that oxytetracycline has no effect on the bone apposition rate when used in the concentrations indicated. In addition, we found that at labeling intervals of 96 h or more, periods of osteoblastic inactivity are likely to be included in measurements at individual sites. The instantaneous apposition rate is thus underestimated at these long time intervals.

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“…After incubation, the tissues were washed in D-MEM with 10% fetal calf serum, and the epithelium was dissected using fine tungsten needles. The mesenchyme was placed on membrane filters supported by metal grids according to the Trowel technique as modified by Saxén (Trowel, 1959;Saxén, 1966). It is important that the mandible mesenchyme is placed so that the oral surface is facing upward.…”

Section: Culturesmentioning

confidence: 99%