Effect of testosterone on the differentiation control of stromal vascular cells isolated from longissimus muscle of Hanwoo beef cattle

Baatar, Delgerzul; Hwang, Seong Gu

doi:10.1016/j.meatsci.2019.107916

Cited by 7 publications

(4 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, SVCs mostly contains adipogenic precursor known as adipose derived stem cells (ASCs) [11]. The SVCs used in current study were analyzed using surface markers and confirmed characterized ASCs in our previous published work [12].…”

Section: Discussionmentioning

confidence: 92%

“…Thus, high-dose GH treatment suppressed adipogenic differentiation and lipid accumulation in both myogenic Regardless of castration timing, blood testosterone level, another important factor that influences marbling score, drops after castration. Our previous study showed that, low levels of testosterone also had increased adipocyte differentiation in SVC but exposure to high levels of testosterone may have negative effect on marbling in late castrated calves [12]. Next, in vivo experiment was conducted to investigate whether the results of the cellular study showing marked differences in the effect of GH levels were also reflected in calves with different castration timing.…”

Section: A C C E T E Dmentioning

confidence: 99%

See 1 more Smart Citation

The difference of castration timing of Korean Hanwoo bulls does not significantly affect the carcass characteristics

Hong

Baatar²,

Hwang

2021

J Anim Sci Technol

Self Cite

View full text Add to dashboard Cite

It is already well known that castration improves marbling quality but exact timing of castration is still highly debated in beef cattle production industry. After castration, blood hormonal changes occur in steer and objective of this study was to investigate the effects of growth hormone (GH) levels on adipocyte differentiation in stromal vascular cells (SVCs) and transdifferentiation into adipocytes in C2C12 myoblasts. Total GH concentrations were measured via enzyme-linked immunosorbent assay (ELISA) in 24 male calves and 4 female calves. Cell proliferation, cellular triglyceride (TG) accumulation, and the cell’s lipolytic capability were measured in C2C12 myoblasts and SVCs. Myogenic, adipogenic, and brown adipocyte-specific gene expression was measured via real-time polymerase chain reaction (PCR) using SYBR green. Serum GH levels were the highest in late-castrated calves. Treatment with 5 ng/mL GH resulted in greater TG accumulation as well as increased CCAAT-enhancer-binding protein (C/EBP)α and peroxisome proliferator-activated receptor (PPAR) γ expression compared to that after treatment with 15 ng/mL GH. Treatment with 5 ng/mL GH also resulted in lower myogenin (myo)G and myoD expression compared to that after treatment with 15 ng/mL GH. The expression of bone morphogenetic protein (BMP) 7 after treatment with 5 ng/mL GH was higher than that after treatment with 15 ng/mL GH. But carcass characteristics data showed no significant difference between early and late castrated steers. Therefore, our results indicate that castration timing does not seem to be inevitable determinate of carcass qualities, particularly carcass weight and marbling score in Hanwoo beef cattle.

show abstract

Section: Discussionmentioning

confidence: 92%

Section: A C C E T E Dmentioning

confidence: 99%

The difference of castration timing of Korean Hanwoo bulls does not significantly affect the carcass characteristics

Hong

Baatar²,

Hwang

2021

J Anim Sci Technol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Additionally established in the scientific literature, males demonstrated to be heavier at weaning [ 72 ]. This response may be associated with the greater muscle fiber quantity in males [ 89 ], which is trigged by testosterone’s effects on the mesenchymal stem cells’ commitment [ 90 ]. Moreover, greater testosterone levels in males also promote greater anabolism for this group once the protein accretion is triggered by testosterone [ 91 ].…”

Section: Discussionmentioning

confidence: 99%

The Effects of Prenatal Diet on Calf Performance and Perspectives for Fetal Programming Studies: A Meta-Analytical Investigation

Barcelos

Nascimento

Silva

et al. 2022

Animals

View full text Add to dashboard Cite

This meta-analysis aimed to identify knowledge gaps in the scientific literature on future fetal-programming studies and to investigate the factors that determine the performance of beef cows and their offspring. A dataset composed of 35 publications was used. The prenatal diet, body weight (BW), average daily gain (ADG) during pregnancy, and calf sex were elicited as possible modulators of the beef cows and their offspring performance. Then, the correlations between these variables and the outcomes of interest were investigated. A mixed multiple linear regression procedure was used to evaluate the relationships between the responses and all the possible explanatory variables. A knowledge gap was observed in studies focused on zebu animals, with respect to the offspring sex and the consequences of prenatal nutrition in early pregnancy. The absence of studies considering the possible effects promoted by the interactions between the different stressors’ sources during pregnancy was also detected. A regression analysis showed that prenatal diets with higher levels of protein improved the ADG of pregnant beef cows and that heavier cows give birth to heavier calves. Variations in the BW at weaning were related to the BW at birth and calf sex. Therefore, this research reinforces the importance of monitoring the prenatal nutrition of beef cows.

show abstract

“…The findings were in agreement with the principle that testosterone deficiency contributes to the increment in lipid droplets in skeletal muscle [ 31 ] and improves adipocyte differentiation rather than that of myocytes in pluripotent mesenchymal stem cells through androgen-receptor-dependent signaling [ 32 ]. Moreover, a high content of testosterone inhibited preadipocyte preparation in and between intramuscular bundle in stromal vascular cells [ 33 ]. Therefore, castration would still be an effective way to produce meat with a high level of marbling.…”

Section: Discussionmentioning

confidence: 99%

Multi-Omics Analysis of Transcriptomic and Metabolomics Profiles Reveal the Molecular Regulatory Network of Marbling in Early Castrated Holstein Steers

Sun

Piao

Zhang

et al. 2022

Animals

View full text Add to dashboard Cite

The intramuscular fat (IMF), or so-called marbling, is known as potential determinant of the high quality beef in China, Korea, and Japan. Of the methods that affect IMF content in cattle, castration is markedly regarded as an effective and economical way to improve the deposition of IMF but with little attention to its multi-omics in early-castrated cattle. The aim of this study was to investigate the liver transcriptome and metabolome of early-castrated Holstein cattle and conduct a comprehensive analysis of two omics associated with the IMF deposition using transcriptomics and untargeted metabolomics under different treatments: non−castrated and slaughtered at 16 months of age (GL16), castrated at birth and slaughtered at 16 months of age (YL16), and castrated at birth and slaughtered at 26 months of age (YL26). The untargeted metabolome was analyzed using ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. The transcriptome of the hepatic genes was analyzed to identify marbling-related genes. Using untargeted metabolomics, the main altered metabolic pathways in the liver of cattle, including those for lipid and amino acid metabolism, were detected in the YL16 group relative to the GL16 and YL26 groups. Significant increases in the presence of betaine, alanine, and glycerol 3-phosphate were observed in the YL16 group (p < 0.05), which might have contributed to the improved beef-marbling production. Compared to the GL16 and YL26 groups, significant increases in the presence of glutathione, acetylcarnitine, and riboflavin but decreases in diethanolamine and 2-hydroxyglutarate were identified in YL16 group (p < 0.05), which might have been beneficial to the beef’s enhanced functional quality. The gene expressions of GLI1 and NUF2 were downregulated and that of CYP3A4 was upregulated in the YL16 group; these results were strongly correlated with the alanine, betaine, and leucine, respectively, in the liver of the cattle. In conclusion, implementation of early castration modified the hepatic metabolites and the related biological pathways by regulating the relevant gene expressions, which could represent a better rearing method for production of high marbled and healthier beef products.

show abstract

Effect of testosterone on the differentiation control of stromal vascular cells isolated from longissimus muscle of Hanwoo beef cattle

Cited by 7 publications

References 32 publications

The difference of castration timing of Korean Hanwoo bulls does not significantly affect the carcass characteristics

The difference of castration timing of Korean Hanwoo bulls does not significantly affect the carcass characteristics

The Effects of Prenatal Diet on Calf Performance and Perspectives for Fetal Programming Studies: A Meta-Analytical Investigation

Multi-Omics Analysis of Transcriptomic and Metabolomics Profiles Reveal the Molecular Regulatory Network of Marbling in Early Castrated Holstein Steers

Contact Info

Product

Resources

About