Effect of testosterone on lipolysis in human pre-adipocytes from different fat depots

Dicker, Andrea; Rydén, Mikael; Näslund, Erik; Muehlen, I. E.; Wirén, Mikael; Lafontan, Max; Arner, Peter

doi:10.1007/s00125-003-1324-0

Cited by 146 publications

(125 citation statements)

References 37 publications

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“…No association was observed with lipolysis, although only basal lipolysis was examined. Other measures of lipolysis, including hormone-sensitive lipase activity and lipolytic sensitivity to adrenergic agonists, have been shown to be modulated by sex hormones (2,11). Their association with AKR1C enzyme expression and activities remains to be determined.…”

Section: Discussionmentioning

confidence: 99%

Expression and activity of steroid aldoketoreductases 1C in omental adipose tissue are positive correlates of adiposity in women

Blouin

Blanchette

Richard

et al. 2005

American Journal of Physiology-Endocrinology and Metabolism

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Section: Discussionmentioning

confidence: 99%

Expression and activity of steroid aldoketoreductases 1C in omental adipose tissue are positive correlates of adiposity in women

Blouin

Blanchette

Richard

et al. 2005

American Journal of Physiology-Endocrinology and Metabolism

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“…Western blot analysis Differentiation of human preadipocytes was performed as previously described by Dicker et al 12 Briefly, cells were isolated from the stromavascular fraction of collagenasetreated adipose tissue obtained from obese, otherwise healthy donors. Cells were differentiated in 22 mm wells using a defined serum-free culture medium supplemented with 10 mmol/l rosiglitazone and 0.2 mmol/l 3-isobutyl-1-methylxanthine (IBMX) for 3 days and maintained in culture medium without rosiglitazone and IBMX until the end of differentiation.…”

Section: Real-time Quantitative Pcrmentioning

confidence: 99%

“…10,11 This could reflect reduced oestrogen levels and/or a relative increase in testosterone, following cessation of oestrogen production in the ovaries. 12 A role of oestrogen in maintenance of body weight is supported by the observation that central adiposity is prevented in women given hormone replacement treatment (HRT). [13][14][15] Furthermore, lower body weight and waist circumference were also seen after treatment with oestrogen and progestin compared to placebo in the Women's Health Initiative Study.…”

Section: Introductionmentioning

confidence: 99%

Oestrogen receptor α gene expression levels are reduced in obese compared to normal weight females

et al. 2007

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Objective: To determine whether oestrogen receptor (ER)a messenger RNA (mRNA) levels or single nucleotide polymorphisms (SNPs) are associated with obesity in Swedish women. Design: ERa mRNA expression levels were measured by real-time qPCR in subcutaneous adipose tissue from non-obese (N ¼ 16, BMI o30) and obese (N ¼ 17, BMI X30) women. In addition, ERa mRNA expression levels were determined in isolated adipocytes. ERa promoter usage was characterized by 5 0 RACE and by real-time qPCR in subcutaneous adipose tissue from the same non-obese and obese women. Two ERa SNPs were scored in 509 non-obese and 489 obese females. Results: ERa mRNA expression levels were lower in obese compared to non-obese women in both subcutaneous adipose tissue and in adipocytes. We show that two ERa promoters are differentially utilized in obese and non-obese individuals. We did not find any significant association between obesity and the ERa SNPs or haplotypes assayed. Conclusion: The reduced ERa mRNA levels observed in adipose tissue from obese compared to non-obese women support a role for oestrogen signaling via ERa, in control of body weight. Mechanistic studies of the role of ERa in adipocytes and how its expression is regulated in relation to fat mass should be performed. The latter studies should focus on the two promoters that are used differently in obese and non-obese individuals.

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“…15 The fat tissue was cut into small pieces and washed several times in phosphatebuffered saline immediately after arrival to the laboratory and incubated in 0.5 g l À1 of collagenase (Sigma, St Louis, MO, USA) in Krebs Ringer phosphate buffer (pH 7.4) with 40 g l À1 of dialyzed bovine serum albumin (BSA, fraction V, Sigma) for 1 h at 37 1C in shaking bath. The disaggregated adipose tissue was filtered through a nylon mesh with a pore size of 250 mm.…”

Section: Isolation and Differentiation Of Preadipocytesmentioning

confidence: 99%

“…Western blot analysis was carried out as described previously. 15 A total of 50 mg of total protein was loaded on 12% polyacrylamide gels and separated by standard SDS-PAGE. Proteins were transferred onto a PVDF transfer membrane (Hybond-P, GE-Healthcare, Amersham Place, Little Challfont, UK).…”

Section: Western Blot Experimentsmentioning

confidence: 99%

Effects of pain controlling neuropeptides on human fat cell lipolysis

et al. 2010

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Objective: Neuropeptides NPFF and NPSF are involved in pain control, acting through the G-protein coupled receptors (GPR)74 (high affinity for NPFF) and GPR147 (equal affinity for NPFF and NPSF). GPR74 also inhibits catecholamine-induced adipocyte lipolysis and regulates fat mass in humans. The aim of this study was to compare the effects of NPFF and NPSF on noradrenalineinduced lipolysis and to determine the expression of their receptors in human fat cells. Design: Adipose tissue was obtained during surgery. Adipocytes were prepared and kept in primary culture. Lipolysis, protein expression and gene expression were determined. Results: NPFF counteracted noradrenaline-induced lipolysis, which was more marked after 48 h than after 4 h exposure and was solely attributed to inhibition of b-adrenoceptor signalling. NPSF counteracted noradrenaline-induced lipolysis maximally after 4 h of exposure, which was attributed to a combination of inhibition of b-adrenoceptor signalling and decreased activation of the protein kinase-A hormone sensitive lipase complex by cyclic AMP. Both neuropeptides were effective in nanomolar concentrations. NPFF and NPSF had no effects on the expression of genes involved in catecholamine signal transduction. Both GPR74 and GPR147 were expressed at the protein level in fat cells from various adipose regions. GPR74 mRNA levels were higher in adipose tissue from obese as compared with non-obese subjects. High gene expression of either receptor correlated with low noradrenaline-induced lipolysis (Po0.05). Conclusions: Pain controlling neuropeptides NPFF and NPSF may be important for the regulation of lipolysis in man probably acting through GPR74 and GPR147. At low concentrations they inhibit catecholamine-induced lipolysis through rapid and longterm post-transcriptional effects at several steps in adrenoceptor signalling in fat cells.

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Effect of testosterone on lipolysis in human pre-adipocytes from different fat depots

Cited by 146 publications

References 37 publications

Expression and activity of steroid aldoketoreductases 1C in omental adipose tissue are positive correlates of adiposity in women

Expression and activity of steroid aldoketoreductases 1C in omental adipose tissue are positive correlates of adiposity in women

Oestrogen receptor α gene expression levels are reduced in obese compared to normal weight females

Effects of pain controlling neuropeptides on human fat cell lipolysis

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