Effect of Testosterone, Estradiol-17β, and 5α-Dihydrotestosterone on Inhibin Production by Sertoli Cells in Culture

Nagendranath, N; Jose, Tessey; Sheth, A. R.; Juneja, H. S.

doi:10.3109/01485018208990242

Cited by 9 publications

(5 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unlike in immature animals, in adults 10 ng DHT may increase the release of FSH-RF from day 82 onwards, resulting in raised serum FSH levels. On the other hand, DHT inhibits the release of inhibin by Sertoli cells in vitro (Nagendranath, Jose, Sheth & Juneja, 1982a). If this observation is con¬ sidered in relation to the present findings, it may be suggested that, unlike in immature animals, lOng DHT inhibits endogenous levels of inhibin from days 82 to 117, resulting in raised serum FSH levels.…”

Section: Discussioncontrasting

confidence: 40%

Ontogeny of serum and pituitary gonadotrophins in male rats treated with low doses of 5α-dihydrotestosterone

Karanth¹,

gill²,

Dutt³

et al. 1986

Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

The effect of s.c. daily injections of 10 or 1000 ng 5 alpha-dihydrotestosterone (DHT)100 g body weight from birth to day 21, or from days 26 to 117 of age, on the changes in concentration of serum and pituitary gonadotrophins was investigated in male rats. Treatment with 10 ng DHT from days 1 to 21 depressed serum FSH, but not LH, at day 7, while 1000 ng DHT depressed both serum LH and FSH. Treatment with both doses of DHT reduced pituitary levels of LH and FSH at day 7, with FSH being more depressed than LH. Treatment with 10 ng DHT from days 26 to 117 increased serum FSH from days 82 to 117, while 1000 ng DHT did not have this effect. Treatment with 1000 ng, but not 10 ng, DHT between days 26 and 117 reduced pituitary levels of LH and FSH at day 40. Rats treated with the two doses of DHT from days 26 to 117 showed a difference in the responsiveness of the pituitary to LH-releasing hormone (LHRH). Treatment with 10 ng DHT enhanced LHRH-induced release of LH without affecting FSH release, while 1000 ng DHT depressed LHRH-induced release of FSH but not of LH. These findings support the view that DHT may play a modulatory role in the ontogeny of serum gonadotrophins and the responsiveness of the pituitary to LHRH during the onset of puberty in the male rat.

show abstract

Section: Discussioncontrasting

confidence: 40%

Ontogeny of serum and pituitary gonadotrophins in male rats treated with low doses of 5α-dihydrotestosterone

Karanth¹,

gill²,

Dutt³

et al. 1986

Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

show abstract

“…This was particularly evident by the fact that studies from some laboratories showed a stimulatory effect of FSH on bioactive inhibin (10)(11)(12)(13)(14) while those from others showed no effect (14). Similarly, testosterone (T) was reported to stimulate (14) or suppress bioactive inhibin (15,16).…”

mentioning

confidence: 87%

Inhibin Production by Primary Sertoli Cell-Enriched Cultures: Regulation by Follicle-Stimulating Hormone, Androgens, and Epidermal Growth Factor*

et al. 1988

View full text Add to dashboard Cite

Inhibin levels in the serum-free media of primary Sertoli cell-enriched (SCE) cultures were studied as a function of time and hormonal treatment. SCE cultures were established from 20-day-old rats and maintained in SF media supplemented with insulin, transferrin, epidermal growth factor (EGF), and bacitracin. Radioimmunoassayable inhibin was measured using an antibody directed against a synthetic porcine inhibin-a (pla) and measured using this same synthetic peptide as well as a highly purified ovine inhibin standard. Results of these determinations are expressed in terms of a synthetic peptide as femtomoles of pIa-(l-26)-G-Y-per ml/10 6 cells. Inhibin levels (±SEM) that accumulated at this rate in media from control cultures were 184.9 ± 6.1 and 167.4 ± 5.2 on days 0-4 and 4-8, respectively. When FSH (oFSH 17; 1-1000 ng/ml) was added, a dose-dependent increase in inhibin levels was significant at all time points beyond the initial 24 h. The simultaneous addition of 2 X 10" 7 M testosterone (T) with low doses of FSH suppressed the inhibin response to FSH, but when T was combined with 300 ng/ml FSH, there was no effect of T on inhibin levels compared to FSH alone, regardless of time in culture. In spite of the modest effect of T, androstenedione (A; 2 X 10" 13 to x 10 5 M) produced a dose-dependent suppression of inhibin levels. This inhibition was also observed at all doses of FSH. The action of A was not due to its conversion to estrogens, as 17/3-estradiol had no effect on inhibin production by SCE cultures in either the presence or absence of FSH. The effect of EGF, a component of the basal serum-free medium, was next examined; it produced a 1.5-fold higher level of inhibin (188.7 ± 9.5) compared to cultures without EGF (135.6 ± 5.0). When SCE cultures were plated with FSH plus EGF, the stimulation of inhibin levels was additive. We conclude that in Sertoli cell cultures established from immature rats (1) the accumulation of inhibin in medium declines from 90 fmol/10 6 cells-day (initial 24 h) to 40-50 fmol/10 6 cells-day (over the first 48 h) and continues to accumulate in the medium for 8 days of culture; (2) FSH regulates the production of inhibin by Sertoli cells; the best dose response is observed over a 3-day period; (3) has a more marked suppressive effect on inhibin accumulation than does T; and (4) EGF stimulates basal inhibin production, and its effects are additive with those of FSH. (Endocrinology 122: [717][718][719][720][721][722][723][724][725] 1988)

show abstract

“…These results provided indirect evidence for the hypothesis that inhibin is involved in the control of FSH secretion. However, the hormonal regulation of inhibin in vivo has not been investigated, although several in-vitro studies employ¬ ing Sertoli cells in culture have been reported (Steinberger, 1979(Steinberger, , 1981Nagendranath, Jose, Sheth & Juneja, 1982;Verhoeven & Franchimont, 1983). Recently we have validated an in-vitro bioassay using cultured rat anterior pituitary cells for the determination of inhibin content in rat testes (Au et al 1983).…”

Section: Introductionmentioning

confidence: 97%

Effects of hypophysectomy and subsequent FSH and testosterone treatment on inhibin production by adult rat testes

Au¹,

Robertson²,

Kretser³

1985

Journal of Endocrinology

View full text Add to dashboard Cite

The hormonal control of inhibin production by adult rat testes was investigated using an in-vitro inhibin bioassay validated for the measurement of inhibin activity in charcoal-treated rat testicular extracts. The effect of hypophysectomy examined at 16 h, 3, 7 and 42 days after surgery showed a decrease in testicular inhibin content and seminiferous tubule fluid production by 7 days and a decrease in inhibin production by 42 days. Serum FSH and LH were suppressed 3 days after surgery. In 30-day chronically hypophysectomized adult rats treated for 3 days with twice daily s.c. injections of (a) human FSH (hFSH, 22 i.u./rat per day), (b) testosterone (5 mg/rat per day), (c) hFSH + testosterone (same doses as a and b), or (d) human chorionic gonadotrophin (hCG, 12 i.u./rat per day), hFSH or hFSH and testosterone stimulated an increase in testicular inhibin content but not in inhibin production or tubule fluid production. Testosterone and hCG had no effect on these parameters. It is concluded that in vivo, FSH alone stimulates an increase in testicular inhibin content. The failure to observe an increase in inhibin production in vivo is attributed to the suppression of seminiferous tubule fluid production under the same experimental conditions.

show abstract

Effect of Testosterone, Estradiol-17β, and 5α-Dihydrotestosterone on Inhibin Production by Sertoli Cells in Culture

Cited by 9 publications

References 7 publications

Ontogeny of serum and pituitary gonadotrophins in male rats treated with low doses of 5α-dihydrotestosterone

Ontogeny of serum and pituitary gonadotrophins in male rats treated with low doses of 5α-dihydrotestosterone

Inhibin Production by Primary Sertoli Cell-Enriched Cultures: Regulation by Follicle-Stimulating Hormone, Androgens, and Epidermal Growth Factor*

Effects of hypophysectomy and subsequent FSH and testosterone treatment on inhibin production by adult rat testes

Contact Info

Product

Resources

About