Effect of testosterone deficiency on cholesterol metabolism in pigs fed a high-fat and high-cholesterol diet

Cai, Zhaowei; Hao, Xi Shan; Pan, Yongming; Jiang, Xiaoling; Chen, Liang; Cai, Yan; Zhu, Kun; Chen, Cheng; Xu, Xiaoping; Chen, Minli

doi:10.1186/s12944-015-0014-5

Cited by 47 publications

(39 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We also found significant differences in serum high density 38 who found that the levels of total cholesterol and LDL cholesterol are increased significantly by castration.…”

Section: Discussionsupporting

confidence: 51%

Effects of Testosterone Replacement on Insulin Sensitivity, Blood Glucose, Serum Lipids and Vitamin D Concentration in a Rat Model of Andropause

Rezk¹

2017

IJCEMS

View full text Add to dashboard Cite

Abstract:Background: Testosterone facilitates various metabolic processes in the bones, muscles, bone marrow, brain and immune system. Testosterone deficiency may contribute to cardiovascular diseases, central obesity and metabolic dysfunction. Aim: to investigate the effects of testosterone deficiency on blood glucose levels, insulin sensitivity, vitamin D, Homeostatic model assessment of insulin resistance (HOMA-IR), Homeostatic model assessment for beta function (HOMA-IR) and serum lipids in rat models of andropause. Methods, 48 male rats divided into 4 equal groups (n=12/group); a) Control (C) group. b) Orchiectomy (O) group underwent bilateral removal of the testicles. c) Orchiectomy and treated (O+T) group given daily IM testosterone replacement therapy for 7 days, d) Sham and treated (Sh+T) group in which sham operated rats were given the same daily dose of T as O+T group for 7 days. Results: we found that plasma glucose concentration increased significantly (P<0.001) in O group. This increase was corrected by testosterone injection in O+T group. In (Sh+T) group, there is a significant reduction in serum glucose (P<0.001). We also found that insulin level reduced significantly (P<0.001) in O group. This reduction in insulin level was corrected by testosterone. In (Sh+T) group, insulin level increased significantly. 25-OHVD3 was reduced significantly (P<0.05) in O group and increased significantly in O+T group. HOMA-IR level decreased significantly (P<0.001) and this reduction was corrected by Testosterone. HOMA-β reduced significantly (P<0.001) and this reduction was corrected by Testosterone in O+T group. In SH group, there is a marked increase in HOMA-β. We also found insignificant differences between the four groups in serum triglycerides. However, we found very highly significant differences in total cholesterol, LDL and VLDL between C group and O group and very highly significant differences between O+T group and Sh+T group in comparison with O group. We also found significant differences in serum HDL-c in (O) group in comparison with (C) group and between O+T and Sh+T groups in comparison with O group. In conclusion, we found significant reduction in 25-OH(VD), Testosterone, Insulin, HOMA-IR and HOMA-B and significant increase in glucose serum levels in O group and these changes were corrected by Testosterone injection in O+T group. We also found insignificant differences between the four groups in serum triglycerides. However, we found highly significant differences in total cholesterol, LDL, HDL-c and VLDL between C group and O group and very highly significant differences between O+T group and Sh+T group in comparison with O group.

show abstract

“…We also found significant differences in serum high density 38 who found that the levels of total cholesterol and LDL cholesterol are increased significantly by castration.…”

Section: Discussionsupporting

confidence: 51%

Effects of Testosterone Replacement on Insulin Sensitivity, Blood Glucose, Serum Lipids and Vitamin D Concentration in a Rat Model of Andropause

Rezk¹

2017

IJCEMS

View full text Add to dashboard Cite

show abstract

“…Also, animal studies have displayed marked increase in serum TC levels in testosteronedeficient male mice (Hatch et al, 2012). In addition, testosterone depletion led to increase in TC, TG and LDL-c levels in animals (Cai et al, 2015). Interestingly, present study displayed dose dependent decrease in the serum testosterone level (data not shown) accompanied with markedly enhanced concentrations of lipid profile (TC, TG and LDL-c) in FLU-treated rats.…”

Section: Discussionsupporting

confidence: 39%

Exploring the protective effect of silymarin against flutamide-induced hepatotoxicity in male rats

2016

Res. Opin. Anim. Vet. Sci.

View full text Add to dashboard Cite

The current study was designed to explore the protective impact of silymarin (SIL) against the hepatotoxicity induced by daily treatment with flutamide (FLU), for one month, in intact male rats. Two different doses of FLU, one was low and corresponds to the human therapeutic dose (10 mg/kg) and the other was high and corresponds to ½ lowest lethal dose (500 mg/kg) were applied. Obtained results showed that oral administration of FLU in male rats significantly increased hepatic malonic dialdehyde (MDA), and markedly decreased hepatic reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-stranseferase (GST). In parallel, activities of alanine aminotranseferase (ALT), aspartate aminotranseferase (AST) and alkaline phosphatase (ALP), and level of total bilirubin in serum were significantly elevated; while serum total proteins, albumin and globulin, and hepatic total proteins were decreased in FLU-treated animals. Also, significant increases in serum and liver contents of total lipids (TL), total cholesterol (TC) and triglycerides (TG), and serum low density lipoproteins-cholesterol (LDL-c), which accompanied with decrease in serum level of high density lipoproteinscholesterol (HDL-c) following administration of FLU. The adverse effects of FLU on measured parameters appeared to be dose-dependent. Treatment with SIL (50 mg/kg) in rats given FLU (10 mg or 500 mg/kg) greatly improved the levels of investigated biomarkers of liver status. However, the beneficial effect of SIL was more remarkable in rats receiving FLU at a dose of 10 mg/kg. Obtained biochemical changes induced by the two used doses of FLU and the ameliorative impact of SIL were confirmed by histopathological examinations of liver sections of treated rats. In conclusion, SIL can be used effectively in the protection against hepatotoxicity induced by FLU.

show abstract

“…It is likely that an adverse intrauterine environment is better able to affect the neuroendocrine programming of lipid metabolic-related genes of female foetuses in utero . This gender difference may be related to sex hormones, and the different effects of oestrogen and androgen on hepatic cholesterol metabolism might play important roles [42, 43]. For example, low oestrogen levels can cause hypercholesterolemia by inhibiting the expression of hepatic CYP7A1 [43].…”

Section: Discussionmentioning

confidence: 99%

Effects and Interactions of Prenatal Ethanol Exposure, a Post-Weaning High-Fat Diet and Gender on Adult Hypercholesterolemia Occurrence in Offspring Rats

Qi¹,

Luo²,

Hu³

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Prenatal ethanol exposure (PEE) could induce intrauterine programming of hypothalamic-pituitary-adrenal axis-associated neuroendocrine metabolism, resulting in intrauterine growth retardation and susceptibility to adult hypercholesterolemia in offspring. This study aimed to analyse the effects and interactions of PEE, a post-weaning high-fat diet (HFD) and gender on the occurrence of adult hypercholesterolemia in offspring rats. Methods: Wistar female rats were treated with ethanol (4 g/kg.d) at gestational days 11-20. The offspring were given a normal diet or HFD after weaning, and the blood cholesterol metabolism phenotype and expression of hepatic cholesterol metabolism related genes were detected in 24-week-old offspring. Furthermore, the interactions among PEE, HFD, and gender on hypercholesterolemia occurrence were analysed. Results: PEE increased the serum total cholesterol (TCH) and low-density lipoprotein-cholesterol (LDL-C) levels and decreased the serum high-density lipoprotein-cholesterol (HDL-C) level in adult offspring rats; the changes in female offspring were greater than those in males. At the same time, the mRNA expression levels of hepatic cholesterol metabolic enzymes (apolipoprotein B (ApoB) and 7α-hydroxylase (CYP7A1))—were increased, while the mRNA expression levels of the scavenger receptor B1 (SR-B1) and LDL receptor (LDLR) were decreased. Furthermore, a three-way ANOVA showed there were interactions among PEE, post-weaning HFD and gender. For PEE offspring, a post-weaning HFD aggravated the elevated hepatic ApoB and CYP7A1 expression and reduced SR-B1 and LDLR expression; the changes in hepatic SR-B1 and CYP7A1 expression were greater in female HFD rats than in males. Conclusion: Our findings suggest that a post-weaning HFD could aggravate offspring hypercholesterolemia caused by PEE and that this mechanism might be associated with hepatic cholesterol metabolic disorders that are aggravated by a post-weaning HFD; hepatic cholesterol metabolism was more sensitive to neuroendocrine metabolic alterations by PEE and a post-weaning HFD in the female offspring than in the male offspring.

show abstract

Effect of testosterone deficiency on cholesterol metabolism in pigs fed a high-fat and high-cholesterol diet

Cited by 47 publications

References 48 publications

Effects of Testosterone Replacement on Insulin Sensitivity, Blood Glucose, Serum Lipids and Vitamin D Concentration in a Rat Model of Andropause

Effects of Testosterone Replacement on Insulin Sensitivity, Blood Glucose, Serum Lipids and Vitamin D Concentration in a Rat Model of Andropause

Exploring the protective effect of silymarin against flutamide-induced hepatotoxicity in male rats

Effects and Interactions of Prenatal Ethanol Exposure, a Post-Weaning High-Fat Diet and Gender on Adult Hypercholesterolemia Occurrence in Offspring Rats

Contact Info

Product

Resources

About