Effect of testosterone and hypoxia on the expansion of umbilical cord blood CD34+ cells in vitro

Zhou, Liping; Zhang, Xiaowei; Zhou, Panpan; Xue, Li; Xu, Xuejing; Shi, Qi; Li, Dong; Ju, Xiuli

doi:10.3892/etm.2017.5026

Cited by 5 publications

(4 citation statements)

References 42 publications

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“…We found that decreased erythropoiesis that results from ADTinduced profound hypogonadism is likely due to reduced stimulation of marrow erythroid precursors. Indeed, pluripotent bone marrow stem cells have androgen receptors, and stimulation by testosterone leads to differentiation of these cells toward erythroid lineage (34,50,59,70). Therefore, unliganded androgen receptor, during ADT, likely inhibits this differentiation.…”

Section: Discussionmentioning

confidence: 99%

Mechanisms responsible for reduced erythropoiesis during androgen deprivation therapy in men with prostate cancer

Gagliano‐Jucá

Pencina

Ganz

et al. 2018

American Journal of Physiology-Endocrinology and Metabolism

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Androgen deprivation therapy (ADT) is a mainstay of treatment for prostate cancer (PCa). As androgens stimulate erythropoiesis, ADT is associated with a reduction in hematocrit, which in turn contributes to fatigue and related morbidity. However, the mechanisms involved in ADT-induced reduction in erythropoiesis remain unclear. We conducted a 6-mo prospective cohort study and enrolled men with PCa about to undergo ADT (ADT-Group) and a control group of men who had previously undergone prostatectomy for localized PCa and were in remission (Non-ADT Group). All participants had normal testosterone levels at baseline. Fasting blood samples were collected at baseline, 12 wk, and 24 wk after initiation of ADT; samples were obtained at the same intervals from enrollment in the Non-ADT group. Blood count, iron studies, erythropoietin, erythroferrone, and hepcidin levels were measured. Seventy participants formed the analytical sample (31 ADT, 39 Non-ADT). ADT was associated with a significant reduction in erythrocyte count (estimated mean difference = −0.2×106cells/µl, 95%CI = −0.3 to −0.1×106cells/µl, P < 0.001), hematocrit (−1.9%, 95%CI = −2.7 to −1.1%, P < 0.001), and hemoglobin (−0.6 g/dl, 95%CI = −0.8 to −0.3 g/dl, P < 0.001). Serum hepcidin concentration increased in the ADT-group (18 ng/ml, P < 0.001); however, iron concentrations did not change (−1.1 µg/dl, P = 0.837). Ferritin levels increased in men on ADT (60 ng/ml, P < 0.001). Iron binding capacity, transferrin saturation, erythroferrone, and erythropoietin did not change. Nine men undergoing ADT developed new-onset anemia. In conclusion, reduced proliferation of marrow erythroid progenitors leads to ADT-induced reduction in erythropoiesis. Future studies should evaluate the role of selective androgen receptor modulators in the treatment of ADT-induced anemia.

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Section: Discussionmentioning

confidence: 99%

Mechanisms responsible for reduced erythropoiesis during androgen deprivation therapy in men with prostate cancer

Gagliano‐Jucá

Pencina

Ganz

et al. 2018

American Journal of Physiology-Endocrinology and Metabolism

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“…During the recent decades, many evidences have been provided on the neuroendocrinology of testosterone in relation to brain activities, cognitive, emotional, and environmental behavior functions, sexual functions, mental health, suicidal behaviors, aging and pathophysiology of mood disorders, psychosis and aggression unobtrusively, and the testosterone's deficiency that may negatively affect the quality of life. [ 10 24 25 26 ] However, a few studies have evaluated the relationship between testosterone levels and bipolar disorder.…”

Section: Discussionmentioning

confidence: 99%

Comparison of salivary testosterone levels in different phases of bipolar I disorder and control group

Mousavizadegan

Maroufi

2018

J Res Med Sci

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Background:Testosterone is considered as a primary sex hormone, also known as an important anabolic steroid, that may involve in various mental disorders such as bipolar I disorder (BID). The goal of this study was to compare the testosterone salivary levels between different phases of BID and its association with the clinical features of BID.Materials and Methods:In a case–control study, 15 patients in the mania phase, 10 patients in the depression phase, and 16 in the euthymia phase were selected as patient groups. 18 healthy sex- and age-matched individuals were considered as healthy control group. Salivary samples obtained from all patients and control group and levels of testosterone were determined in saliva using an enzyme-linked immunosorbent assay. All statistical calculations were conducted with the software Statistical Package for Social Science version 20 (IBM Inc., Chicago, IL, USA).Results:The mean testosterone level in euthymia phase was 186.34 ± 182.62 pg/mL, mania phase was 239.29 ± 273.22 pg/mL, depression was 153.49 ± 222.50 pg/mL, and healthy participants was 155.73 ± 126.0 pg/mL; no significant difference was found between groups (P = 0.68.(No statistically significant differences were found between psychotic and nonpsychotic as well as between patients who attempted suicide and nonattempter patients in terms of testosterone levels (P > 0.1).Conclusion:Our findings do not reveal significant difference between different phases of BID in terms of salivary testosterone levels. However, more comprehensive studies with larger sample size are required to confirm our findings.

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“…26 Di sisi lain, hormon testosteron juga diketahui memiliki efek stimulasi pada aktivitas fungsional HSCs, meningkatkan proliferasi dan mobilisasinya dengan melibatkan transkripsi gen homeobox (HOX)A9, HOXB2, HOXB4, HOXC4 dan BMI-1. 28 Estradiol juga memiliki aktivitas yang sama dengan testosteron dalam meningkatkan aktivitas HSCs yaitu dengan meningkatkan aktivitas telomerase melalui induksi ekspresi gen TERT sehingga masa hidup HSCs lebih panjang. 29 Sel punca hematopoietik memiliki molekul yang khas pada permukaan selnya, yaitu molekul glikoprotein CD34 + .…”

Section: Bahasan Pelatihan Fisik Seimbang Meningkatkan Jumlah Hscsunclassified

Pelatihan Fisik Berlebih Menurunkan Jumlah Hematopoietic Stem Cells (HSCs) Dibandingkan Pelatihan Fisik Seimbang pada Tikus (Rattus norvegicus) Wistar Jantan

Zenitalia¹,

Pangkahila²,

Pangkahila³

et al. 2018

JBM

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This study was aimed to prove that balanced physical exercise increased mobilization of hematopoietic stem cells (HSCs) compared to excessive physical exercise in male Wistar rats (Rattus norvegicus). This was an experimental study with the randomized pretest-posttest control group design. Subjects were 24 male Wistar rats (Rattus norvegicus), 2.5-3 months old, weighing 180-200 g, healthy (active and did not show any anatomical abnormality), divided into two groups of 12 rats each. One group (P0) was treated with balanced physical exercise and the other group (P1) was treated with excessive physical exercise. Before (pretest) and 4 weeks after treatment (posttest), peripheral blood of 1 ml was drawn through medial canthus sinus orbitalis for examination of HSCs number in peripheral blood quantitatively. The results showed that the number of HSCs in the P0 group was increased from 1.60±0.70 x 106 cell/μl before treatment to 2.70±0.62 x 106 cells/μl after 4-week treatment (P <0.05). Meanwhile, in the P1 group, the number of HSCs was decreased from 1.74±0.68 x 106 cells/μl before treatment to 1.34±0.55 x 106 cells/μl after 4-week treatment (P <0.05). Conclusion: Excessive physical exercise decreased number of hematopoietic stem cells (HSCs) compared to balanced physical exercise in male Wistar rats (Rattus norvegicus).Keywords: balanced physical exercise, excessive physical exercise, hematopoietic stem cells (HSCs)Abstrak: Tujuan penelitian ini ialah untuk membuktikan bahwa pelatihan fisik berlebih menurunkan jumlah hematopoietic stem cells (HSCs) dibandingkan pelatihan fisik seimbang pada tikus (Rattus norvegicus) Wistar jantan. Jenis penelitian ini ialah eksperimental dengan randomized pre-post test control group design. Subyek penelitian ialah 24 ekor tikus Wistar (Rattus norvegicus) jantan, umur 2,5-3 bulan, berat badan 180-200 gr, sehat (aktif dan tidak menunjukkan abnormalitas anatomi) yang dibagi menjadi dua kelompok yaitu P0 dan P1, masing-masing berjumlah 12 ekor. Kelompok P0 diberikan pelatihan seimbang dan kelompok P1 diberi pelatihan fisik berlebih. Sebelum (pretest) dan 4 minggu setelah perlakuan (posttest), darah diambil sebanyak 1 ml melalui medial canthus sinus orbitalis untuk pemeriksaan kadar HSCs darah tepi secara kuantitatif. Hasil penelitian menunjukkan bahwa pada kelompok P0 terjadi peningkatan jumlah HSCs dari 1,60±0,70 x106 sel/μl pretest menjadi 2,70±0,62 x106 sel/μl posttest (P <0,05). Pada kelompok P1 terjadi penurunan jumlah HSCs dari 1,74±0,68 x106 sel/μl pretest menjadi 1,34±0,55 x106 sel/μl posttest (P <0,05). Simpulan: Pelatihan fisik berlebih menurunkan jumlah hematopoietic stem cells (HSCs) dibandingkan pelatihan fisik seimbang pada tikus (Rattus norvegicus) Wistar jantan.Kata kunci: pelatihan fisik seimbang, pelatihan fisik berlebih, hematopoietic stem cells (HSCs)

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Effect of testosterone and hypoxia on the expansion of umbilical cord blood CD34+ cells in vitro

Cited by 5 publications

References 42 publications

Mechanisms responsible for reduced erythropoiesis during androgen deprivation therapy in men with prostate cancer

Mechanisms responsible for reduced erythropoiesis during androgen deprivation therapy in men with prostate cancer

Comparison of salivary testosterone levels in different phases of bipolar I disorder and control group

Pelatihan Fisik Berlebih Menurunkan Jumlah Hematopoietic Stem Cells (HSCs) Dibandingkan Pelatihan Fisik Seimbang pada Tikus (Rattus norvegicus) Wistar Jantan

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