Effect of temperature, pressure and lipid acyl chain length on the structure and phase behaviour of phospholipid–gramicidin bilayers

Zein, M.; Winter, Roland

doi:10.1039/b003565n

Cited by 82 publications

(74 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In such a dynamic situation, the significance of the observed conformation would be unclear. However, at room temperature the DSPC membrane is in its gel phase (30), and static disorder on molecular scales is expected to play a prominent role. It is possible that the observed dimer conformation -an anisotropic structure-is strongly influenced by the shapes and sizes of free volume pockets that form spontaneously inside the amphiphilic membrane domain.…”

Section: Resultsmentioning

confidence: 99%

Conformation of self-assembled porphyrin dimers in liposome vesicles by phase-modulation 2D fluorescence spectroscopy

Lott

Perdomo-Ortiz

Utterback

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

112

160

View full text Add to dashboard Cite

By applying a phase-modulation fluorescence approach to 2D electronic spectroscopy, we studied the conformation-dependent exciton coupling of a porphyrin dimer embedded in a phospholipid bilayer membrane. Our measurements specify the relative angle and separation between interacting electronic transition dipole moments and thus provide a detailed characterization of dimer conformation. Phase-modulation 2D fluorescence spectroscopy (PM-2D FS) produces 2D spectra with distinct optical features, similar to those obtained using 2D photon-echo spectroscopy. Specifically, we studied magnesium meso tetraphenylporphyrin dimers, which form in the amphiphilic regions of 1,2-distearoyl-sn-glycero-3-phosphocholine liposomes. Comparison between experimental and simulated spectra show that although a wide range of dimer conformations can be inferred by either the linear absorption spectrum or the 2D spectrum alone, consideration of both types of spectra constrain the possible structures to a "T-shaped" geometry. These experiments establish the PM-2D FS method as an effective approach to elucidate chromophore dimer conformation.fluorescence-detected 2D photon echo | nonlinear spectroscopy | supramolecular conformation | excitonically coupled dimer T he ability to determine three-dimensional structures of macromolecules and macromolecular complexes plays a central role in the fields of molecular biology and material science. Methods to extract structural information from experimental observations such as X-ray crystallography, NMR, and optical spectroscopy are routinely applied to a diverse array of problems, ranging from investigations of biological structure-function relationships to the chemical basis of molecular recognition.In recent years, two-dimensional optical methods have become well established to reveal incisive information about noncrystalline macromolecular systems-information that is not readily obtainable by conventional linear spectroscopic techniques. Twodimensional optical spectroscopy probes the nanometer-scale couplings between vibrational or electronic transition dipole moments of neighboring chemical groups, similar to the way NMR detects the angstrom-scale couplings between adjacent nuclear spins in molecules (1). For example, 2D IR spectroscopy probes the couplings between local molecular vibrational modes and has been used to study the structure and dynamics of mixtures of molecular liquids (2), aqueous solutions of proteins (3), and DNA (4). Similarly, 2D electronic spectroscopy (2D ES) probes correlations of electronic transitions and has been used to study the mechanisms of energy transfer in multichromophore complexes. Such experiments have investigated the details of femtosecond energy transfer in photosynthetic protein-pigment arrays (5-8), conjugated polymers (9), and semiconductors (10, 11).Following the examples established by 2D NMR and 2D IR, 2D ES holds promise as a general approach for the structural analysis of noncrystalline macromolecular systems, albeit for the nanometer length scales ...

show abstract

Section: Resultsmentioning

confidence: 99%

Conformation of self-assembled porphyrin dimers in liposome vesicles by phase-modulation 2D fluorescence spectroscopy

Lott

Perdomo-Ortiz

Utterback

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

112

160

View full text Add to dashboard Cite

show abstract

“…The conformation of gramicidin, a low-molecular-weight peptide antibiotic, is modulated by phase transitions of the lipid matrix. Incorporation of the peptide leads to significant changes in the structure and pressure-temperature phase behavior of the lipid bilayer system (52).…”

Section: Discussionmentioning

confidence: 99%

“…As a general rule, oligomeric proteins are dissociated at relatively low pressure (Ͻ200 MPa), whereas the irreversible denaturation of enzymes and proteins in aqueous solution requires pressures higher than 300 MPa (21,28). Few data are available on pressure-induced structural changes of proteins dissolved in membranes (52).…”

mentioning

confidence: 99%

Effects of Pressure-Induced Membrane Phase Transitions on Inactivation of HorA, an ATP-Dependent Multidrug Resistance Transporter, in Lactobacillus plantarum

Ulmer

Herberhold

Fahsel

et al. 2002

Appl Environ Microbiol

Self Cite

107

View full text Add to dashboard Cite

The effects of pressure on cultures of Lactobacillus plantarum were characterized by determination of the viability and activity of HorA, an ATP-binding cassette multidrug resistance transporter. Changes in the membrane composition of L. plantarum induced by different growth temperatures were determined. Furthermore, the effect of the growth temperature of a culture on pressure inactivation at 200 MPa was determined. Cells were characterized by plate counts on selective and nonselective agar after pressure treatment, and HorA activity was measured by ethidium bromide efflux. Fourier transform-infrared spectroscopy and Laurdan fluorescence spectroscopy provided information about the thermodynamic phase state of the cytoplasmic membrane during pressure treatment.

show abstract

“…The major advantage of using D 2 O is a shift in the deformation band of water, which prevents the band from overlapping with the amide I' band and facilitates the analysis of the hydration state of the amide C = O groups. [10] The amide I' region consists mainly of the C=O stretching vibration with a minor contribution of NÀH stretching. As the amide I' region is very sensitive to hydrogen bonding, we focus on this band.…”

mentioning

confidence: 99%

Interplay between Hydrogen Bonding and Macromolecular Architecture Leading to Unusual Phase Behavior in Thermosensitive Microgels

et al. 2007

View full text Add to dashboard Cite

Effect of temperature, pressure and lipid acyl chain length on the structure and phase behaviour of phospholipid–gramicidin bilayers

Cited by 82 publications

References 34 publications

Conformation of self-assembled porphyrin dimers in liposome vesicles by phase-modulation 2D fluorescence spectroscopy

Conformation of self-assembled porphyrin dimers in liposome vesicles by phase-modulation 2D fluorescence spectroscopy

Effects of Pressure-Induced Membrane Phase Transitions on Inactivation of HorA, an ATP-Dependent Multidrug Resistance Transporter, in Lactobacillus plantarum

Interplay between Hydrogen Bonding and Macromolecular Architecture Leading to Unusual Phase Behavior in Thermosensitive Microgels

Contact Info

Product

Resources

About