Effect of temperature on the <i>Arabidopsis</i> cryptochrome photocycle

Pooam, Marootpong; Dixon, Nykiera; Hilvert, Michael; Misko, Peter; Waters, Kristy; Jourdan, Nathalie; Drahy, Soria; Mills, Stephen A.; Engle, Dorothy; Link, Justin; Ahmad, Margaret

doi:10.1111/ppl.13365

Cited by 20 publications

(16 citation statements)

References 21 publications

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“…Moreover, these light intensities correspond to natural conditions that plants encounter in winter (Robson and Aphalo, 2019 ). Furthermore, photoreceptors of blue and red light are sufficiently activated at this intensity (Park et al, 2012 ; Liu et al, 2020b ), and reduced temperature prolongs their activated state (Legris et al, 2016 ; Pooam et al, 2021 ). The action spectrum for photosynthesis is comparable for the blue and red light components, and approximately the same amount of energy was available for photosynthesis in our experiment.…”

Section: Discussionmentioning

confidence: 99%

Light Quality Modulates Plant Cold Response and Freezing Tolerance

Kameniarová

Černý²,

Novák³

et al. 2022

Front. Plant Sci.

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The cold acclimation process is regulated by many factors like ambient temperature, day length, light intensity, or hormonal status. Experiments with plants grown under different light quality conditions indicate that the plant response to cold is also a light-quality-dependent process. Here, the role of light quality in the cold response was studied in 1-month-old Arabidopsis thaliana (Col-0) plants exposed for 1 week to 4°C at short-day conditions under white (100 and 20 μmol m−2s−1), blue, or red (20 μmol m−2s−1) light conditions. An upregulated expression of CBF1, inhibition of photosynthesis, and an increase in membrane damage showed that blue light enhanced the effect of low temperature. Interestingly, cold-treated plants under blue and red light showed only limited freezing tolerance compared to white light cold-treated plants. Next, the specificity of the light quality signal in cold response was evaluated in Arabidopsis accessions originating from different and contrasting latitudes. In all but one Arabidopsis accession, blue light increased the effect of cold on photosynthetic parameters and electrolyte leakage. This effect was not found for Ws-0, which lacks functional CRY2 protein, indicating its role in the cold response. Proteomics data confirmed significant differences between red and blue light-treated plants at low temperatures and showed that the cold response is highly accession-specific. In general, blue light increased mainly the cold-stress-related proteins and red light-induced higher expression of chloroplast-related proteins, which correlated with higher photosynthetic parameters in red light cold-treated plants. Altogether, our data suggest that light modulates two distinct mechanisms during the cold treatment - red light-driven cell function maintaining program and blue light-activated specific cold response. The importance of mutual complementarity of these mechanisms was demonstrated by significantly higher freezing tolerance of cold-treated plants under white light.

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Section: Discussionmentioning

confidence: 99%

Light Quality Modulates Plant Cold Response and Freezing Tolerance

Kameniarová

Černý²,

Novák³

et al. 2022

Front. Plant Sci.

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“…However, there were five GhCRY genes which were induced after a 12-h low temperature treatment, along with the repression of an equal number of the GhCRY s. These results demonstrated the functional divergence among the CRYs in G. hirsutum . Plant cryptochromes have been implicated in adaptations to the changing environmental factors, for instance, a report showed that low temperatures would increase the biological activity of CRY [ 62 ]. Consistently, as reported herein in cotton each CRYs may behave differently in response to low temperature stresses.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive identification and expression analysis of CRY gene family in Gossypium

Huang

Cao

et al. 2022

BMC Genomics

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Background The cryptochromes (CRY) are specific blue light receptors of plants and animals, which play crucial roles in physiological processes of plant growth, development, and stress tolerance. Results In the present work, a systematic analysis of the CRY gene family was performed on twelve cotton species, resulting in 18, 17, 17, 17, and 17 CRYs identified in five alloteraploid cottons (Gossypium hirsutum, G. barbadense, G. tomentosum, G. mustelinum and G. darwinii), respectively, and five to nine CRY genes in the seven diploid species. Phylogenetic analysis of protein-coding sequences revealed that CRY genes from cottons and Arabidopsis thaliana could be classified into seven clades. Synteny analysis suggested that the homoeolog of G. hirsutum Gh_A02G0384 has undergone an evolutionary loss event in the other four allotetraploid cotton species. Cis-element analysis predicated the possible functions of CRY genes in G. hirsutum. RNA-seq data revealed that Gh_D09G2225, Gh_A09G2012 and Gh_A11G1040 had high expressions in fiber cells of different developmental states. In addition, the expression levels of one (Gh_A03G0120), 15 and nine GhCRY genes were down-regulated following the PEG, NaCl and high-temperature treatments, respectively. For the low-temperature treatment, five GhCRY genes were induced, and five were repressed. These results indicated that most GhCRY genes negatively regulate the abiotic stress treatments. Conclusion We report the structures, domains, divergence, synteny, and cis-elements analyses systematically of G. hirsutum CRY genes. Possible biological functions of GhCRY genes in differential tissues as well as in response to abiotic stress during the cotton plant life cycle were predicted.

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“…Consistent with temperature control of CRY biological activity and protein stability Ma et al, 2021;Pooam et al, 2021), CRYs play important roles in temperature-regulated plant growth and development. For example, it was reported two decades ago that vegetative phyB cry1 mutant plants exhibited temperature-dependent internode elongation (Mazzella et al, 2000).…”

Section: Blue Light-mediated Temperature Responsesmentioning

confidence: 97%

“…It was proposed that FAD is in the inactive oxidized state (FADox) in the dark; upon absorbing B light, FAD is reduced to the redox state (FADH°) that is considered to be the active form of CRYs (Ahmad, 2016; Hammad et al, 2020). A recent report showed that the dark reversion rate from FADH° to FADox was lower at 15°C than at 25°C, suggesting that low ambient temperatures causes higher biological activity of CRYs by increasing the concentrations of the active FADH° form (Pooam et al, 2021). The molecular mechanisms underlying the temperature responses of CRYs remain currently obscure.…”

Section: The Roles Of Light Signaling Components In Temperature Respo...mentioning

confidence: 99%