Effect of temperature on the formation and inactivation of syringomycin E pores in human red blood cells and bimolecular lipid membranes

Agner, Gabriella; Kaulin, Yuri A.; Schagina, Ludmila V.; Takemoto, Jon Y.; Blaskó, Katalin

doi:10.1016/s0005-2736(00)00173-5

Cited by 14 publications

(6 citation statements)

References 44 publications

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“…This conductance is approximately two orders of magnitude lower than the conductance calculated from Ohm's law if the channel radius is taken to be equal to 1 nm. The 1-nm estimate for the channel radius was obtained by at least three different methods (Schagina et al, 1998;Kaulin et al, 1998;Dalla Serra et al, 1999;Agner et al, 2000). A similar large discrepancy between the measured and calculated values of the channel conductance was reported for colicin E1 (Raymond et al, 1985;Kayalar and Duzgunes, 1986;Slatin, 1988, Bullock et al, 1992 and, recently, for colicin Ia channels (Krasilnikov et al, 1998).…”

Section: Resultsmentioning

confidence: 70%

Syringomycin E Channel: A Lipidic Pore Stabilized by Lipopeptide?

Malev

Schagina

Gurnev

et al. 2002

Biophysical Journal

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Highly reproducible ion channels of the lipopeptide antibiotic syringomycin E demonstrate unprecedented involvement of the host bilayer lipids. We find that in addition to a pronounced influence of lipid species on the open-channel ionic conductance, the membrane lipids play a crucial role in channel gating. The effective gating charge, which characterizes sensitivity of the conformational equilibrium of the syringomycin E channels to the transmembrane voltage, is modified by the lipid charge and lipid dipolar moment. We show that the type of host lipid determines not only the absolute value but also the sign of the gating charge. With negatively charged bilayers, the gating charge sign inverts with increased salt concentration or decreased pH. We also demonstrate that the replacement of lamellar lipid by nonlamellar with the negative spontaneous curvature inhibits channel formation. These observations suggest that the asymmetric channel directly incorporates lipids. The charges and dipoles resulting from the structural inclusion of lipids are important determinants of the overall energetics that underlies channel gating. We conclude that the syringomycin E channel may serve as a biophysical model to link studies of ion channels with those of lipidic pores in membrane fusion.

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Section: Resultsmentioning

confidence: 70%

Syringomycin E Channel: A Lipidic Pore Stabilized by Lipopeptide?

Malev

Schagina

Gurnev

et al. 2002

Biophysical Journal

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“…Although interesting antifungal properties have mostly emerged from work relating to crop protection and biocontrol, they could also present an important therapeutic relevance as antifungal drugs. Unfortunately, purified Ps-CLPs can be haemolytic, such as reported for massetolide A-(V) ( Van De Mortel et al, 2009 ), WLIP-(V) ( Andolfi et al, 2008 ), orfamide A ( Henkels et al, 2014 ), arthrofactin ( Morikawa et al, 1993 ), tolaasins ( Rainey et al, 1991 ), syringomycin E ( Agner et al, 2000 ), and syringopeptins 22A-B ( Hutchinson and Gross, 1997 ). Despite this, a single injection of 10 mg kg -1 of massetolide A was reported to be non-toxic to mice ( Gerard et al, 1997 ).…”

Section: Pseudomonas Spp Produce a Large Variety Of Clpsmentioning

confidence: 99%

Cyclic Lipodepsipeptides From Pseudomonas spp. – Biological Swiss-Army Knives

2018

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Cyclic lipodepsipeptides produced by Pseudomonas spp. (Ps-CLPs) are biosurfactants that constitute a diverse class of versatile bioactive natural compounds with promising application potential. While chemically diverse, they obey a common structural blue-print, allowing the definition of 14 distinct groups with multiple structurally homologous members. In addition to antibacterial and antifungal properties the reported activity profile of Ps-CLPs includes their effect on bacterial motility, biofilm formation, induced defense responses in plants, their insecticidal activity and anti-proliferation effects on human cancer cell-lines. To further validate their status of potential bioactive substances, we assessed the results of 775 biological tests on 51 Ps-CLPs available from literature. From this, a fragmented view emerges. Taken as a group, Ps-CLPs present a broad activity profile. However, reports on individual Ps-CLPs are often much more limited in the scope of organisms that are challenged or activities that are explored. As a result, our analysis shows that the available data is currently too sparse to allow biological function to be correlated to a particular group of Ps-CLPs. Consequently, certain generalizations that appear in literature with respect to the biological activities of Ps-CLPs should be nuanced. This notwithstanding, the data for the two most extensively studied Ps-CLPs does indicate they can display activities against various biological targets. As the discovery of novel Ps-CLPs accelerates, current challenges to complete and maintain a useful overview of biological activity are discussed.

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“…There are several studies devoted to the comparative evaluation of 16S-23S rRNA intergenic spacer regions in P. syringae и P. fluorescens and to the rep-PCR analysis of a limited number of Pseudomonas strains isolated from cereal crops [16, 17]. Using MLST, Ignatov et.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of draft genomes of two closely related pseudomonas syringae phylogroup 2b strains infecting mono- and dicotyledon host plants

et al. 2016

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BackgroundIn recent years, the damage caused by bacterial pathogens to major crops has been increasing worldwide. Pseudomonas syringae is a widespread bacterial species that infects almost all major crops. Different P. syringae strains use a wide range of biochemical mechanisms, including phytotoxins and effectors of the type III and type IV secretion systems, which determine the specific nature of the pathogen virulence.ResultsStrains 1845 (isolated from dicots) and 2507 (isolated from monocots) were selected for sequencing because they specialize on different groups of plants. We compared virulence factors in these and other available genomes of phylogroup 2 to find genes responsible for the specialization of bacteria. We showed that strain 1845 belongs to the clonal group that has been infecting monocots in Russia and USA for a long time (at least 50 years). Strain 1845 has relatively recently changed its host plant to dicots.ConclusionsThe results obtained by comparing the strain 1845 genome with the genomes of bacteria infecting monocots can help to identify the genes that define specific nature of the virulence of P. syringae strains.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3358-y) contains supplementary material, which is available to authorized users.

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Effect of temperature on the formation and inactivation of syringomycin E pores in human red blood cells and bimolecular lipid membranes

Cited by 14 publications

References 44 publications

Syringomycin E Channel: A Lipidic Pore Stabilized by Lipopeptide?

Syringomycin E Channel: A Lipidic Pore Stabilized by Lipopeptide?

Cyclic Lipodepsipeptides From Pseudomonas spp. – Biological Swiss-Army Knives

Comprehensive analysis of draft genomes of two closely related pseudomonas syringae phylogroup 2b strains infecting mono- and dicotyledon host plants

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