Effect of temperature on the compositions of ladderane lipids in globally surveyed anammox populations

Kouba, Vojtěch; Hurkova, Kamila; Navrátilová, Klára; Vejmelková, Dana; Benáková, Andrea; Laureni, Michele; Vodickova, P.; Podzimek, T.; Lipovová, Petra; Niftrik, Laura van; Hajšlová, Jana; Loosdrecht, M.C.M. van; Weissbrodt, David G.; Bartáček, Jan

doi:10.1016/j.scitotenv.2022.154715

Cited by 11 publications

(5 citation statements)

References 27 publications

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“…Possibly, C16 alkyls might have been branched and their reduction might further restrict diffusion. In the polar part of lipids, respectively, the relative share of each phosphatidylcholine (FC), phosphatidylethanolamine (FE), or phosphatidylglycerol (FG) did not show a clear trend with increasing temperature varying from 0.21 to 0.55, which is consistent with the results of Kouba et al (2022c), Kouba et al (2022b) and Kouba et al (2022d), as each of these studies found a different polar head group dominating at lower temperatures. Similarly, the ester-to-ether ratio fluctuated from 0.05 to 0.16 without a link to temperature, again correspondingly to the divergencies in previous literature (Kouba et al, 2022c;Rattray et al, 2010).…”

Section: Non-ladderane Structural Alterationssupporting

confidence: 84%

“…The samples for ladderane analysis were sampled at the end of each Run after at least 5 days of operation of R1 at 40 °C and simultaneously from R0 (30 °C) with a dried weight of 0.3 g. The sample preparation and analysis were done according to Kouba et al (2022b) by lyophilizing the samples and subsequently shaking them in an extraction solvent mixture (MeOH: DCM, 2:1, v/v). Afterwards, the samples were sonicated, centrifuged (5 min, 10,000 rpm, 5 °C) and filtered through a 90 μm filter paper, grade 390 (Ahlstrom, Munksjö).…”

Section: Ladderane Analysismentioning

confidence: 99%

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Mechanisms of Anammox Adaptation to High Temperatures: Increased Cyclization of Ladderane Lipids and Proteomic Insights

Christina,

Klára,

Adam

et al. 2024

Preprint

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Although anammox-based processes have been widely applied in mesophilic conditions of reject water and recently in mainstream conditions, the potential of their implementation in high-temperature wastewaters remains largely unexplored. Therefore, this study investigated the operation parameters for the successful adaptation of anammox bacteria and the mechanisms involved on the proteomic and cellular level including unique ladderane lipids. For this purpose, the enrichment of Candidatus Brocadia was cultivated in two fed-batch reactors (FBRs) at a lab scale. The temperature of one FBR was gradually increased from 30 to 40 degrees C while the other FBR was maintained at 30 degrees C with four consecutive replicates of this experiment. For this adaptation to be successful, the original loading rate had to be at least halved, or ideally maintained below half the value of the specific anammox activity at the time. The most notable adaptation mechanisms included: (1) upregulation of chaperones and (2) doubled ladderane cyclization via the replacement of non-ladderane fatty acid by a ladderane fatty acid in ladderane lipids (p-value 0.005). To our best knowledge, this is the first study to describe the novel mechanism of ladderane cyclization which together with other adaptation strategies presents crucial indicators in anammox adaptation to high-temperature wastewaters.

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Section: Non-ladderane Structural Alterationssupporting

confidence: 84%

Section: Ladderane Analysismentioning

confidence: 99%

Mechanisms of Anammox Adaptation to High Temperatures: Increased Cyclization of Ladderane Lipids and Proteomic Insights

Christina,

Klára,

Adam

et al. 2024

Preprint

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“…4). Finally, the following triterpenoids were identified in anammox enrichments (ordered from lowest to highest abundance): squalene, bacteriohopanetetrol, and bacteriohopanetetrol cyclitol ether (for more details refer to Kouba et al [49]).…”

Section: Ladderane Compositionmentioning

confidence: 99%

“…Scalindua" under low temperatures raises inquiry into their membrane physiology. As described in more detail in Kouba et al [49], the [3]-ladderanes (Ea15-30 = 51 kJ. mol − 1 , C20/(C18 + C20) [3]-ladderane ether = 0.11; Fig.…”

Section: Anammox Performance Of Various Genera: Potential Of Marine "...mentioning

confidence: 99%

On anammox activity at low temperature: Effect of ladderane composition and process conditions

Kouba

Hurkova

Navrátilová

et al. 2022

Chemical Engineering Journal

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“…With further research, metabolomics has been widely introduced into the study of disease mechanisms, biomarkers, tissue development regulation, and other related molecular mechanisms ( Chevrette et al, 2019 ; Modoux et al, 2020 ; Chen et al, 2022 ). Metabolomics is a comprehensive analysis of metabolites in specific biological systems using high throughput mass spectrometry and nuclear magnetic resonance, among which UPLC-HRMS is the most promising metabolomics analysis tool internationally recognized to date due to its high accuracy and resolution and the simultaneous analysis of multiple indicators ( Kouba et al, 2022 ; Pan et al, 2022 ). Untargeted metabolomics combined with biological epigenetic observation is used to detect the dynamic changes of many metabolites before and after stimulation or disturbance, to find metabolites with different expressions, and then clarify the metabolic processes of organisms ( Snyder et al, 2013 ; Bode and Challinor, 2015 ; Kim et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Metabonomics reveals that entomopathogenic nematodes mediate tryptophan metabolites that kill host insects

Zhang

Wang²,

Zhao³

2022

Front. Microbiol.

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The entomopathogenic nematode (EPN) Steinernema feltiae, which carries the symbiotic bacterium Xenorhabdus bovienii in its gut, is an important biocontrol agent. This EPN could produce a suite of complex metabolites and toxin proteins and lead to the death of host insects within 24–48 h. However, few studies have been performed on the key biomarkers released by EPNs to kill host insects. The objective of this study was to examine what substances produced by EPNs cause the death of host insects. We found that all densities of nematode suspensions exhibited insecticidal activities after hemocoelic injection into Galleria mellonella larvae. EPN infection 9 h later led to immunosuppression by activating insect esterase activity, but eventually, the host insect darkened and died. Before insect immunity was activated, we applied a high-resolution mass spectrometry-based metabolomics approach to determine the hemolymph of the wax moth G. mellonella infected by EPNs. The results indicated that the tryptophan (Trp) pathway of G. mellonella was significantly activated, and the contents of kynurenine (Kyn) and 3-hydroxyanthranilic acid (3-HAA) were markedly increased. Additionally, 3-HAA was highly toxic to G. mellonella and resulted in corrected mortalities of 62.50%. Tryptophan metabolites produced by EPNs are a potential marker to kill insects, opening up a novel line of inquiry into exploring the infestation mechanism of EPNs.

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Effect of temperature on the compositions of ladderane lipids in globally surveyed anammox populations

Cited by 11 publications

References 27 publications

Mechanisms of Anammox Adaptation to High Temperatures: Increased Cyclization of Ladderane Lipids and Proteomic Insights

Mechanisms of Anammox Adaptation to High Temperatures: Increased Cyclization of Ladderane Lipids and Proteomic Insights

On anammox activity at low temperature: Effect of ladderane composition and process conditions

Metabonomics reveals that entomopathogenic nematodes mediate tryptophan metabolites that kill host insects

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