Effect of Temperature on Ozone Inactivation of
            <b>
              <i>Cryptosporidium parvum</i>
            </b>
            in Oxidant Demand-Free Phosphate Buffer

Li, Hanbin; Gyürék, Lyndon L.; Finch, Gordon R.; Smith, Daniel; Belosevic, Miodrag

doi:10.1061/(asce)0733-9372(2001)127:5(456)

Cited by 36 publications

(49 citation statements)

References 34 publications

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“…However, ozone inactivation of microorganisms in water has been reported to be even higher at higher temperatures. Ozone inactivation of Giardia lamblia at pH 7 (45), Naegleria gruberi at pH 7 (45), Cryptosporidium at pH 6 to 8 (41,42), and Bacillus subtilis spores at pH 7 (48) was higher as temperature increased toward 25°C, which is consistent with ozone inactivation of PrP Sc observed in this study. Inactivation of prions by other oxidants has also been examined in various studies (23,(54)(55)(56)(57)(58)(59)(60)(61).…”

Section: Discussionsupporting

confidence: 81%

Kinetics of Ozone Inactivation of Infectious Prion Protein

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Neumann

Price

et al. 2013

Appl Environ Microbiol

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eThe kinetics of ozone inactivation of infectious prion protein (PrP Sc , scrapie 263K) was investigated in ozone-demand-free phosphate-buffered saline (PBS). Diluted infectious brain homogenates (IBH) (0.01%) were exposed to a predetermined ozone dose (10.8 ؎ 2.0 mg/liter) at three pHs (pH 4.4, 6.0, and 8.0) and two temperatures (4°C and 20°C). The inactivation of PrP Sc was quantified by determining the in vitro destruction of PrP Sc templating properties using the protein misfolding cyclic amplification (PMCA) assay and bioassay, which were shown to correlate well. The inactivation kinetics were characterized by both Chick-Watson (CW) and efficiency factor Hom (EFH) models. It was found that the EFH model fit the experimental data more appropriately. The efficacy of ozone inactivation of PrP Sc was both pH and temperature dependent. Based on the EFH model, CT (disinfectant concentration multiplied by contact time) values were determined for 2-log 10 , 3-log 10 , and 4-log 10 inactivation at the conditions under which they were achieved. Our results indicated that ozone is effective for prion inactivation in ozone-demand-free water and may be applied for the inactivation of infectious prion in prion-contaminated water and wastewater.

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Section: Discussionsupporting

confidence: 81%

Kinetics of Ozone Inactivation of Infectious Prion Protein

Ding

Neumann

Price

et al. 2013

Appl Environ Microbiol

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“…The effect of temperature on the inactivation of microorganisms and the degradation of organic pollutants by ozone have also been investigated by us and others (9,18). In general, elevated temperatures result in a more rapid inactivation of microbial pollutants by ozone, an outcome also observed in the present study.…”

Section: Discussionsupporting

confidence: 74%

“…Ozone as an advanced oxidation technology is widely used in water and wastewater treatment processes for inactivation of bacteria (44), viruses (19,39), and protozoa (18). Although certain advanced oxidation processes have been shown to inactivate infectious prions (14,25,26,34,35), several major knowledge gaps exist; in particular, knowledge concerning how interactions between oxidant reaction conditions (e.g., temperature, pH, duration of exposure, ozone dose, and organic load) affect inactivation levels of prions is lacking.…”

mentioning

confidence: 99%

Inactivation of Template-Directed Misfolding of Infectious Prion Protein by Ozone

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Neumann

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et al. 2012

Appl Environ Microbiol

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Misfolded prions (PrP Sc ) are well known for their resistance to conventional decontamination processes. The potential risk of contamination of the water environment, as a result of disposal of specified risk materials (SRM), has raised public concerns. Ozone is commonly utilized in the water industry for inactivation of microbial contaminants and was tested in this study for its ability to inactivate prions (263K hamster scrapie ‫؍‬ PrP Sc ). Treatment variables included initial ozone dose (7.6 to 25.7 mg/liter), contact time (

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“…Unlike the IGH model, the EFH model enables researchers to describe disinfection kinetics by using mathematical functions available in commonly used computer packages such as Microsoft Excel (Microsoft Corp.) (19). IGH and EFH models have been employed to describe disinfection kinetics in previous studies (17,18,33) and have been used to predict enteric adenovirus type 40 and feline calicivirus inactivation by chlorine (44).…”

Section: Methodsmentioning

confidence: 99%