Effect of Temperature on Ovarian Maturation Stages and Embryonic Development of Mud Spiny Lobster, Panulirus polyphagus

Ikhwanuddi, M.; Fatihah, Siti Nor; Nurfaseha, A.H.; Fathiah, M.; Effenddy, M.; Shamsudin, A.; Aisha, A. Siti; Abol-Munaf, A.B.

doi:10.3923/ajcb.2014.1.13

Cited by 5 publications

(2 citation statements)

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“…The commercial-scale hatchery production of spiny lobster pueruli and juveniles is difficult (Phillips and Kittaka, 2000), and studies of the restocking of spiny lobsters remain limited (Herrnkind et al, 1997;Phillips and Evans, 1997). The predominant problems facing P. polyphagus breeding include low survival rates of the pueruli larval stage, the delicate maintenance of pueruli and juveniles, and difficulty in obtaining mature broodstock, particularly P. polyphagus males (Fatihah et al, 2014a(Fatihah et al, , 2014bIkhwanuddin et al, 2014a). Thus, artificial insemination, discriminating crossbreeding, domestication and conservation of stocks, and sperm cryopreservation techniques will improve the cultivation of P. polyphagus.…”

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confidence: 99%

Development of a sperm cryopreservation protocol for the mud spiny lobster, Panulirus polyphagus

et al. 2016

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This study aimed to develop a cryopreservation protocol for sperm of the mud spiny lobster, Panulirus polyphagus. Sperm of P. polyphagus were successfully cryopreserved using a protocol with cooling periods of 15 min per temperature, 25, 20, 16, 4, 2, −4, −20, −80, and −150°C, followed by immediate storage in liquid nitrogen (at −196°C). The efficacy of the cryopreserved protocol was determined by assessing the viability of sperm. The optimal thawing temperature for cryopreservation of sperm was 26°C for 30 s, with a viability rate of 76.09% ± 7.81. At room temperature, −20 and −80°C, 10% glycine provided the highest percentage of sperm viability at 91.87 ± 2.03% (5 min at room temperature), 91.31 ± 2.65% (6 h at − 20°C) and 75.88 ± 10.81% (6 h at − 80°C). In conclusion, we developed a protocol (Protocol I) for the successful cryopreservation of P. polyphagus sperm using Ca-F saline as an extender and 10% glycine as a cryoprotectant. Statement of relevance: This article is suitable with Aquaculture Journal because the findings will give a big contribution in mud spiny lobster aquaculture. Mud spiny lobster seedling especially P. polyphagus is getting less landing every year, new approach needed in ensuring enough supplies of P. polyphagus to be available. The content in this article would help developing the research innovation for the hatchery production of P. polyphagus to increase.

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Section: Introductionmentioning

confidence: 99%

Development of a sperm cryopreservation protocol for the mud spiny lobster, Panulirus polyphagus

et al. 2016

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“…This lack of protection of spawners could damage reproductive potential of the lobster population, resulting in recruitment failure and overexploitation and the fact, the average size of the lobster population has been decreasing over the last decade (Chang et al, 2007). In P. polyphagus breeding technology, the major problems are low survival rate in the larvae rearing of pueruli stage and hard to maintain from pueruli until juvenile stages (Fatihah et al, 2014a, b;Ikhwanuddin et al, 2014a). In addition, lack of information for the purposes of fisheries management is one of the major constraints in understanding the management and development of mud spiny lobster, P. polyphagus .…”

Section: Introductionmentioning

confidence: 99%

Effect of Water Salinity on Ovarian Maturation Stages and Embryonic Development of Mud Spiny Lobster, Panulirus polyphagus

Ikhwanuddi¹,

Fatihah²,

Nurfaseha³

et al. 2015

J. of Fisheries and Aquatic Science

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A study was carried out to determine the effect of salinity on ovarian maturation stages (external morphology and histology characteristics of the ovary) and embryonic development (time of embryonic development and size of egg) of mud spiny lobster, Panulirus polyphagus. For the ovarian maturation stages, there were three treatments; treatment 1 for 20 ppt (E1T1), treatment 2 for 40 ppt (E1T2) and control treatment for 30 ppt (E1C2) and conducted within 100 days. There was no significant difference (p>0.05) between control in 30 ppt at day 1 (E1C1), E1C2, E1T1and E1T2 noted in the Oocyte Size Index (OSI). Mean oocyte diameter and Gonadosomatic Index (GSI) had significantly differences (p<0.05) between E1C1, E1C2, E1T1 and E1T2. For the embryonic development, the berried female was placed in tank with 20 ppt for treatment 1 (E2T1), 30 ppt for control treatment (E2C1) and 40 ppt for treatment 2 (E2T2) and 20-30 eggs were taken for each salinity to observe the embryonic development. Stage 1, 2 and 3 for 20 ppt and stage 1 for 40 ppt cannot be observed the development of embryo. At low salinity of 20 ppt (E2T1), the shortest duration from stage 4 until hatched was about 4 days while at high salinity of 40 ppt (E2T2), the longest duration from stage 4 until hatched was about 8 days. Thus, the low salinity was the shortest time to hatch. The effect on the sizes changes was stronger in the salinities of 30 and 20 ppt than 40 ppt. Therefore, the high salinity was the lowest egg size compared to low and control treatments. In conclusion, the different salinity affected the ovarian maturation stages and embryonic development of P. polyphagus.

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Ontogenetic variation in digestive enzyme activities within embryos and newly-hatched larvae of the tropical spiny lobster, Panulirus ornatus

et al. 2022

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Effect of Temperature on Ovarian Maturation Stages and Embryonic Development of Mud Spiny Lobster, Panulirus polyphagus

Cited by 5 publications

References 6 publications

Development of a sperm cryopreservation protocol for the mud spiny lobster, Panulirus polyphagus

Development of a sperm cryopreservation protocol for the mud spiny lobster, Panulirus polyphagus

Effect of Water Salinity on Ovarian Maturation Stages and Embryonic Development of Mud Spiny Lobster, Panulirus polyphagus

Ontogenetic variation in digestive enzyme activities within embryos and newly-hatched larvae of the tropical spiny lobster, Panulirus ornatus

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