Effect of temperature on life cycle of entomopathogenic nematode, Heterorhabditis indica poinar

Paschapur, Amit Umesh; Vijayalakshmi, K.; Sunanda, B.S.; Pawar, V. M.

doi:10.15740/has/au/12.techsear(2)2017/578-582

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…Samples of isolated EPNs were stored in portable cool boxes within few hours when transferred to the laboratory of integrated protection, Alexandria or even to the location of field trials. The isolated EPNs were placed in polyurethane foams in darkness at 25 ± 2 o C few days prior to laboratory or semi-field experiments (Ramakuwela et al, 2015;Paschapur et al, 2017).…”

Section: Entomopathogenic Nematodes Culturesmentioning

confidence: 99%

The Humoral Immunological Response and Bio-Efficiency Evaluation to Entomopathogenic Nematodes as A Biopesticide on Spodoptera littoralis (Boisd)

Shahin

Khamis

El-Sabrout

2023

Alexandria Science Exchange Journal

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The current study aims to investigate the residual toxicity and nutritional indices of entomopathogenic nematodes (EPNs), as well as immunological response to an infection in Spodoptera littoralis. Compared to the individual impacts of Steinernema carpocapsae, Heterorhabditis indica and EPNs binary combinations showed a higher pathogenicity to S. littoralis according to mortality, physiological responses, and humoral immune. The anti-feedant activity percentages of H. indica and S. carpocapsae treated larvae had a more potent effect than their binary mixture at LC35, compared to full-feeding activity in the control. The biochemical characteristics of infected hemolymph showed a significant variation, not only in the total protein concentration, but also in the appearance of the new protein bands profile. An additional band, with a molecular weight of ~79 KDa was observed at immunized hemolymph, it has been postulated that it may be the phenoloxidase (PO), which is in close agreement with the significant induction of PO activity after infection. Interestingly, the immunised hemolymph of the larvae infected with binary combinations of EPNs showed a notably increased intensity of the major proteins and a rise in the number of antimicrobial peptides with molecular weights under 30 KDa. However, the significant reduction of total protein count and PO level, which ultimately resulting in larval death, is evidence that the binary mixture of the investigated nematodes is highly suppressed S. Littoralis larval immune system.

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Section: Entomopathogenic Nematodes Culturesmentioning

confidence: 99%

The Humoral Immunological Response and Bio-Efficiency Evaluation to Entomopathogenic Nematodes as A Biopesticide on Spodoptera littoralis (Boisd)

Shahin

Khamis

El-Sabrout

2023

Alexandria Science Exchange Journal

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“…Entomopathogenic nematodes of the genera Steinernema and Heterorhabditis reside in the soil as developmentally arrested dispersal-stage infective juvenile (IJ) larvae (reviewed in Dillman and Sternberg 2012;Schwartz 2015). Upon encountering a suitable insect host, an entomopathogenic nematode invades its body, molts, and resumes development; in the process the nematode releases endosymbiotic pathogenic bacteria from its intestine into its insect host.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of a peroxidase gene controlling body size in the entomopathogenic nematodeSteinernema hermaphroditum

Schwartz,

Tan,

Peraza

et al. 2023

Preprint

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The entomopathogenic nematodeSteinernema hermaphroditumwas recently rediscovered and is being developed as a genetically tractable experimental system for the study of previously unexplored biology, including parasitism of its insect hosts and mutualism with its bacterial endosymbiontXenorhabdus griffiniae. Through whole-genome re-sequencing and genetic mapping we have for the first time molecularly identified the gene responsible for a mutationally defined phenotypic locus in an entomopathogenic nematode. In the process we observed an unexpected mutational spectrum following EMS mutagenesis in this species. We find that the ortholog of the essentialC. elegansperoxidase geneskpo-2controls body size and shape inS. hermaphroditum. We confirmed this identification by inactivating the gene using CRISPR-Cas9. We propose that the identification ofskpo-2will accelerate gene targeting in otherSteinernemaentomopathogenic nematodes used commercially in pest control, asskpo-2is X-linked and males hemizygous for loss of its function can mate, makingskpo-2an easily recognized and maintained marker for use in co-CRISPR.

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Effect of temperature on life cycle of entomopathogenic nematode, Heterorhabditis indica poinar

Cited by 2 publications

References 7 publications

The Humoral Immunological Response and Bio-Efficiency Evaluation to Entomopathogenic Nematodes as A Biopesticide on Spodoptera littoralis (Boisd)

The Humoral Immunological Response and Bio-Efficiency Evaluation to Entomopathogenic Nematodes as A Biopesticide on Spodoptera littoralis (Boisd)

Molecular identification of a peroxidase gene controlling body size in the entomopathogenic nematodeSteinernema hermaphroditum

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