“…Intracellular protein was labeled by a 10‐min pulse of L ‐[ 14 C]leucine (3.7 kBq ml −1 , 5 μM) during exponential growth in SG medium. The labeled culture was centrifuged (5 min, 10 000× g , 4°C), resuspended in fresh ice‐cold SG medium supplemented with L ‐leucine (final concentration: 2 mM) [13], divided in two equal portions, and placed on a water bath shaker (35°C). After a 1‐min temperature equilibration of the resuspended culture, the salt stress was applied in one half of the population.…”