Effect of Temperature in the Evaluation of Yeast Flocculation Ability by the Helm's Method

Gonzalez, M.G.; Fernández, Sergio; Sierra, J. A.

doi:10.1094/asbcj-54-0029

Cited by 3 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The crystals seemed to block the NC tubes preventing cell entrapment. Additionally, cell flocculation caused by low temperature stress [10] , could also block the tubes. Figure 2B illustrates the formation of microbial flocks and proves that the reduction in microbial removal can be attributed to cell flocculation.…”

Section: Resultsmentioning

confidence: 99%

Nano-Tubular Cellulose for Bioprocess Technology Development

et al. 2012

View full text Add to dashboard Cite

Delignified cellulosic material has shown a significant promotional effect on the alcoholic fermentation as yeast immobilization support. However, its potential for further biotechnological development is unexploited. This study reports the characterization of this tubular/porous cellulosic material, which was done by SEM, porosimetry and X-ray powder diffractometry. The results showed that the structure of nano-tubular cellulose (NC) justifies its suitability for use in “cold pasteurization” processes and its promoting activity in bioprocessing (fermentation). The last was explained by a glucose pump theory. Also, it was demonstrated that crystallization of viscous invert sugar solutions during freeze drying could not be otherwise achieved unless NC was present. This effect as well as the feasibility of extremely low temperature fermentation are due to reduction of the activation energy, and have facilitated the development of technologies such as wine fermentations at home scale (in a domestic refrigerator). Moreover, NC may lead to new perspectives in research such as the development of new composites, templates for cylindrical nano-particles, etc.

show abstract

Section: Resultsmentioning

confidence: 99%

Nano-Tubular Cellulose for Bioprocess Technology Development

et al. 2012

View full text Add to dashboard Cite

show abstract

Quantification of Yeast Flocculation

Soares¹,

Mota

1997

Journal of the Institute of Brewing

View full text Add to dashboard Cite

A sedimentation method based in the Helm's flocculation test was improved. In this method, all steps were standardised: initial cell concentration, agitation, sampling and determination off settled cells. The results obtained with the sedimentation test do not differ significantly (P = 0.05) from those obtained with the Stratford test; besides, the precision of the two methods do not differ significantly (P o 0.05). The results presented clearly demonstrated that the method improved in this work is rigorous for quantification of yeast flocculation and allows the differentiation of flocculation ability of the strains.The respective micro-flocculation tests were also compared; Stratford micro-flocculation test was selected as producing the most meaningful results.

show abstract