Effect of Temperature and Ph on Phenol Biodegradation by a Newly Identified Serratia Sp. Aq5-03

Abstract

Phenol is mainly used by the industries to produce a variety of chemical products such as resins, textiles, pesticides, plastics and explosive. The wide use of phenol and other phenolic compounds by industries, has resulted in an increased presence of these toxic compounds in the environment as pollutants. Bio-removal of phenol by microorganisms especially bacteria has been demonstrated to be the most effective and economical approach compared to physio-chemical methods. The search for efficient phenol-degraders especially local sources to remediate local phenol pollution is important as indigenous bacteria usually have better survival and resilient to local geographical conditions. In this study, a phenol-degrading microorganism was isolated from local soil and waste water bodies. Identification was carried out using gram staining, 16s rRNA gene sequencing and molecular phylogeny analysis using the Phylip software. The isolates were inoculated in mineral salt media with 0.5 g/L phenol as the sole source of carbon. Phenol degradation was determined using 4-amino antipyrine method. Physical and cultural conditions influencing phenol degradation such as pH and temperature were optimized via one-factor-at-a-time. Through phylogeny analysis, the isolate was identified as Serratia sp. and the sequence was deposited the NCBI Genebank and accession number KT693287 was assigned to the bacteria. The highest degradation was achieved at pH 7.5 (phosphate buffer) and temperature of 30°C. Ammonium sulphate was established to be the best nitrogen source at the concentration of 0.4 g/L and a sodium chloride concentration of 0.15 g/L.
 Aisami, A. | Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, Selangor, Malaysia

Characterization and Biodegradation of Phenol by Pseudomonas aeruginosa and Klebsiella variicola Strains Isolated from Sewage Sludge and Their Effect on Soybean Seeds Germination

“…The highest phenol degradation by Serratia sp. (accession number KT693287) was achieved at pH 7.5, the temperature of 30 °C and ammonium sulfate as a nitrogen source at the concentration of 0.4 g/L as well as a sodium chloride concentration of 0.15 g/L [ 31 ].…”

“…Additionally, phenol degradation was recorded after four days of incubation in a shaking incubator (120 rpm) at different conditions of temperatures (25, 30, 35, and 40 °C), pH values (6, 7, and 8), and different nitrogen sources in growth medium MSM that contain peptone, yeast extract, urea extract, NaNO 3 , KNO 3 , and NH 4 Cl at a concentration of 2 g/L. Buffer solutions with different pH ranges, i.e., acetate buffer for pH 6.0, phosphate buffer for pH 6.5, 7.0, and 7.5, and Tris-HCl buffer for pH 9 were used to achieve different pH values [ 31 ].…”

“…The highest phenol degradation by Serratia sp. (accession number KT693287) was achieved at pH 7.5, the temperature of 30 °C and ammonium sulfate as a nitrogen source at the concentration of 0.4 g/L as well as a sodium chloride concentration of 0.15 g/L [ 31 ]. Klebsiella oxytoca degraded phenol (100 ppm) within 72 h. In comparison, at a phenol concentration above 400 ppm, the cells could not degrade the substrate efficiently due to the increasing phenol concentration in the medium.…”

Isolation and Characterization of Yarrowia lipolytica YQ22 from Diesel Samples for Phenol Biodegradation

“…Moreover, pH showed a significant difference on the percentage of phenol removal among the experimental assays (P < 0.05) with a value of 0.01. This is because pH affects the physiological and biochemical activities of the microorganism, influencing its growth and proliferation (Aisami et al, 2020). In Fig.…”

Microbiological treatment of post-industrial water: Example of efficient bioremediation of the heavily polluted Kalina pond, Poland

“…For the case of phenol, the highest degradation rates were achieved for Serratia sp. at 30°C (Aisami et al, 2020), Klebsiella oxytoca at 37°C (Mahgoub et al, 2023), and Pseudomonas aeruginosa KBM13 at 40°C (Ghaima, Rahal and Mohamed, 2017).…”