Effect of Temperature and Light on the Stability of Latanoprost and its Clinical Relevance

Morgan, Parham V.; Proniuk, Stefan; Blanchard, James; Noecker, Robert J.

doi:10.1097/00061198-200110000-00007

Cited by 44 publications

(30 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the actual therapeutic efficacy of thermally stressed and/or degraded PGAs remains to be determined. The current data correlate reasonably well with thermal degradation rates for latanoprost previously published by Morgan et al, 29 who reported that latanoprost was stable at 48C and 258C but measured T90s of 8.3 and 1.3 days when stored at 508C and 708C, respectively. Other studies have measured the remaining concentration of PGAs collected directly from glaucoma patients after patient-directed storage with varying results.…”

Section: Discussionsupporting

confidence: 90%

“…Several published studies have assessed the stability 28,29 or efficacy 30,31 of PGAs under conditions of thermal stress or patient storage, and one has compared the stability of patient-stored samples of latanoprost and travoprost. 32 However, to our knowledge, no published study exists comparing the thermal stability of all three PGAs when stored under identical controlled experimental conditions while also simulating normal use by patients.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Thermal Stability of Bimatoprost, Latanoprost, and Travoprost Under Simulated Daily Use

Johnson

Gupta

Vudathala

et al. 2011

Journal of Ocular Pharmacology and Therapeutics

View full text Add to dashboard Cite

Purpose: To determine the stability of bimatoprost, latanoprost, and travoprost under conditions of simulated daily use and varying degrees of thermal stress. Methods: Commercially available bimatoprost, latanoprost, and travoprost were obtained in their original bottles as distributed by the manufacturers. Bottles were stored in calibrated, nonhumidified, light-free incubators maintained at 278C, 378C, or 508C for 3, 9, 15, or 30 days. Capped bottles were inverted and left uncapped for 1 min daily to simulate patient use; no drops were expelled. Bimatoprost concentration was analyzed using liquid chromatography with ultraviolet detection at 210 nm. Latanoprost and travoprost concentrations were analyzed by liquid chromatography/tandem mass spectrometry (MS/MS) using selected reaction monitoring. Results: Off-the-shelf control bottles of bimatoprost contained 102% of the labeled concentration. In all combinations of stress temperature and duration, mean bimatoprost concentration ranged from 100% to 116% of the labeled concentration with no measurable degradation. Off-the-shelf control bottles of latanoprost contained 115% of the labeled concentration. Mean latanoprost concentration ranged from 97% to 120% of the labeled concentration. Latanoprost was stable at 278C. When stressed at 378C or 508C, latanoprost degraded at a rate of 0.15 or 0.29 mg/mL/day, respectively. Off-the-shelf control bottles of travoprost contained 120% of the labeled concentration. Mean travoprost concentration ranged from 83% to 142% of the labeled concentration. Travoprost was stable at 278C and 378C, although concentration measurements at 378C exhibited high variability. When stressed at 508C, travoprost degraded at a rate of 0.46 mg/mL/day. Conclusions: Higher than expected concentrations for stressed drug samples are likely a result of evaporation. Under the conditions of thermal stress tested in this study, bimatoprost remained stable for all conditions tested. Latanoprost degradation was measurable only in samples stressed at 378C and 508C, whereas travoprost degradation was statistically significant only in samples stressed at 508C.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Thermal Stability of Bimatoprost, Latanoprost, and Travoprost Under Simulated Daily Use

Johnson

Gupta

Vudathala

et al. 2011

Journal of Ocular Pharmacology and Therapeutics

View full text Add to dashboard Cite

show abstract

“…The HPLC-UV has been used but was limited to quantify latanoprost at microgram level. Morgan et al is the first to measure 12.2 μg mL −1 of latanoprost (minimum concentration) using this method but gave no information about RT [15]. Sakai et al also used this method to analyze latanoprost but neither the lowest concentration measured nor RT has been reported [16].…”

Section: Methods Optimizationmentioning

confidence: 99%

“…These analytical techniques are: high-performance liquid chromatography (HPLC) equipped with radioactive detectors [8][9][10][11][12][13], diode array detector [14], and UV detector [15][16][17][18][19][20]. The HPLC-UV is the simplest method for the quantification of latanoprost [19] but has longer analysis time (≥3.3 min).…”

Section: Fig 1 Chemical Structure Of Latanoprostmentioning

confidence: 99%

Simple, fast, and sensitive isocratic high-performance liquid chromatography method for the quantification of latanoprost

Mansoor

Taş

2014

Acta Chromatographica

View full text Add to dashboard Cite

Summary.A simple, sensitive, fast, and accurate isocratic high-performance liquid chromatography (HPLC) method with UV detection (205 nm) has been developed and validated for the quantification of latanoprost (in nanogram level) in pure and commercial dosage form. An Agilent Eclipse XDB-C 18 column (150 × 4.6 mm i.d., 3.5 µm particle size) was used. Chromatography was performed with a mobile phase containing a mixture of acetonitrile and water (70:30, v/v) containing 0.1% v/v trifluoroacetic acid, adjusted at pH 3.0 at a flow rate of 1 mL min −1 . Each analysis required 3.0 min including triamcinolone acetonide as an internal standard. The method was validated for linearity, accuracy, precision, specificity, system suitability, and robustness. The calibration curve was linear in the concentration range 0.0125-1 µg mL −1 ; the correlation coefficient was 0.999. The method proved to be accurate, precise, specific, rapid, and reproducible according to ICH standards. The method showed good recoveries (average 100.15 %), and the relative standard deviations of intra-and inter-day were <2.0%. This method is novel since there has been no report about analysis of latanoprost at ng mL −1 levels using a simple HPLC method. Besides regular quality control assay of latanoprost in raw material and in commercial formulations, this method has key importance for scientists working on sustained drug delivery system of latanoprost in which quantification in nanogram levels is one of the most important parameters.

show abstract

“…The recommendation for refrigeration was made based on experimental finding in the laboratory. There was 10% degradation after extreme exposure to 50°C for 198 hours (Morgan et al, 2001). In clinical practice, it is safe to transport and store at room temperature without reducing the effectiveness of the drug (Novak and Evans, 2001;Varma et al, 2006).…”

Section: Topical Latanoprost 0005%mentioning

confidence: 97%

Pressure Lowering Medications

Tajudin¹,

Yaakub²

2011

Glaucoma - Current Clinical and Research Aspects

View full text Add to dashboard Cite

Effect of Temperature and Light on the Stability of Latanoprost and its Clinical Relevance

Abstract: Latanoprost exhibits thermal and solar instability and should ideally be stored below room temperature and in the dark. The importance of these storage conditions should be conveyed clearly to the patient.

Cited by 44 publications

References 4 publications

Thermal Stability of Bimatoprost, Latanoprost, and Travoprost Under Simulated Daily Use

Thermal Stability of Bimatoprost, Latanoprost, and Travoprost Under Simulated Daily Use

Simple, fast, and sensitive isocratic high-performance liquid chromatography method for the quantification of latanoprost

Pressure Lowering Medications

Contact Info

Product

Resources

About