Effect of temperature and diet on wound healing in Atlantic salmon (Salmo salar L.)

Jensen, Linda B.; Wahli, Thomas; McGurk, Charles; Eriksen, Tommy Berger; Obach, Alex; Waagbø, Rune; Handler, Ana; Tafalla, Carolina

doi:10.1007/s10695-015-0105-2

Cited by 55 publications

(61 citation statements)

References 58 publications

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“…Hence, there has been a few studies reporting effects of environmental factors, hormones and dietary components on the healing rate of deep cutaneous wounds. In Atlantic salmon, low water temperature result in delayed epidermal repair 11 , 16 , while the stress hormone cortisol delay the dermal repair processes 12 . In contrast, dietary intake of zinc enhanced epidermal repair in Atlantic salmon 16 , while the dermal repair was promoted in Rainbow trout ( Oncorhynchus mykiss ) with high dietary levels of vitamin C 17 .…”

Section: Introductionmentioning

confidence: 99%

High fish density delays wound healing in Atlantic salmon (Salmo salar)

Sveen

Timmerhaus

Krasnov

et al. 2018

Sci Rep

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In this study, we look closer at how high fish densities influence wound repair mechanisms in post-smolt Atlantic salmon. The fish were wounded with a 5 mm skin punch biopsy needle and stocked at two different densities, a high fish density (100 kg/m3) treatment and a low fish density treatment (20 kg/m3) serving as the control. The healing wounds were followed for 57 days with samples taken 1, 3, 7, 14, 36, 43 and 57 days post wounding. The transcriptomic analysis suggests that high fish density enhance inflammation and represses cell proliferation, tissue secretion and collagen synthesis in the healing wounds. The histological analysis further showed delayed epidermal and dermal repair in the high fish density treatment compared to control. The overall wound contraction was also altered by the treatment. In conclusion, high fish density enhances immune responses and delay tissue repair, which ultimately results in delayed wound healing.

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Section: Introductionmentioning

confidence: 99%

High fish density delays wound healing in Atlantic salmon (Salmo salar)

Sveen

Timmerhaus

Krasnov

et al. 2018

Sci Rep

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“…WE-skin11f is shown here to express vimentin and collagen I, typical of DF, and mhIa and b2m genes, and to respond to ascorbic acid and cortisol. The behaviour of WE-skin11f at different temperatures also was explored because in fish temperature modulates wound healing (Jensen et al, 2015) and immune gene expression (Sever, Vo, Lumsden, Bols, & Dixon, 2014).…”

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confidence: 99%

Characterization of the continuous skin fibroblastoid cell line, WE‐skin11f, from walleye (Sander vitreus)

Katzenback

Kellendonk

et al. 2019

Journal of Fish Diseases

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A walleye dermal fibroblastoid cell line, WE‐skin11f, was established and characterized. WE‐skin11f was immunocytochemically positive for two known dermal fibroblast protein markers: vimentin and collagen I. At passage 26, WE‐skin11f cultures contained both diploid and aneuploid populations. Ascorbic acid was required to produce extracellular collagen I fibres. Both of the skin fibroblastoid cell lines, WE‐skin11f and rainbow trout‐derived RTHDF, were not as good as the walleye caudal fin fibroblastoid cell line, WE‐cfin11f, at forming abundant dense extracellular collagen matrices. The thermobiology of WE‐skin11f was similar to that of other walleye cell lines with 26°C showing best temperature for growth and 4°C showing no growth but 100% viability. The transcript levels of b2m and mhIa genes of the major histocompatibility class I receptor in WE‐skin11f were largely similar at all temperatures examined (4, 14, 20 and 26°C). Cortisol had a variety of effects on WE‐skin11f cells: growth inhibition, morphological change from fibroblastoid to epithelioid, and enhancement of barrier function. Treatment of WE‐skin11f cells with the physiologically relevant concentration of 100 ng/ml cortisol inhibited collagen I synthesis and matrix formation. Thus, WE‐skin11f cell line could be useful in fish dermatology, endocrinology, and immunology research.

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“…Winter ulcer is a temperature dependent disease that occurs at temperature below 8°C [3]. It has also been shown that wound healing in Atlantic salmon occurs faster at 12°C than at 6°C [54]. Similarly, as…”

Section: Discussionmentioning

confidence: 99%

The AinS autoinducer synthase and LitR master regulator of quorum sensing regulate N-3-hydroxy-decanoyl-homoserine-lactone production and motility in Aliivibrio wodanis 06/09/139

Maharajan

Hansen²,

Willassen³

2019

Preprint

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Background Quorum Sensing (QS) is a cell to cell communication system, in which bacteria synthesize and respond to signaling molecules called autoinducers (AI). QS is cell density dependent and known to be involved in regulating virulence, motility and secretion systems to interact with the host or other bacteria. Aliivibrio wodanis is frequently isolated together with Moritella viscosa from the infected Atlantic salmon during outbreaks of the winter ulcer disease. M. viscosa is the main causative agent of the disease while the presence of A. wodanis is still unclear. It is hypothesized that A. wodanis might influence the progression of winter ulcer. The genome of A. wodanis 06/09/139 encodes two autoinducer synthase genes (ainS and luxS) and a master regulator litR. LitR homologs in other aliivibrios have been shown to regulate several phenotypes in a cell density dependent manner. Moreover, a previous study has shown that A. wodanis 06/09/139 produces only one AHL N-3-hydroxy-decanoyl-homoserine-lactone (3OHC10-HSL). Hence, in this work, we have studied the QS system in A. wodanis 06/09/139 by knocking out QS genes ainS and litR. The effects of the deletions were studied with regard to growth, AHL production and motility at different temperatures. Results By using HPLC-MS/MS, we found that the deletion of ainS in A. wodanis 06/09/139 resulted in the loss of 3OHC10-HSL production. The 3OHC10-HSL production in A. wodanis 06/09/139 increased with increase in cell density and more 3OHC10-HSL was produced at 6°C than at 12, 16 and 20°C. The litR mutant demonstrated a ~20% reduction in the production of 3OHC10-HSL relative to the wild type at the stationary phase. Compared to the wildtype and the ainS mutant strains, the litR mutant resulted in a strain with improved temperature tolerance. The motility in mutants (∆litR and ∆ainS) were significantly higher than that of the wildtype. Conclusions Our study shows that AinS in A. wodanis 06/09/139 is the AHL synthase responsible for 3OHC10-HSL production, where the production is both cell density and temperature dependent. Our data also shows that LitR regulates 3OHC10-HSL production only to a minor extent and both LitR and AinS are negative regulators of motility.

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Effect of temperature and diet on wound healing in Atlantic salmon (Salmo salar L.)

Cited by 55 publications

References 58 publications

High fish density delays wound healing in Atlantic salmon (Salmo salar)

High fish density delays wound healing in Atlantic salmon (Salmo salar)

Characterization of the continuous skin fibroblastoid cell line, WE‐skin11f, from walleye (Sander vitreus)

The AinS autoinducer synthase and LitR master regulator of quorum sensing regulate N-3-hydroxy-decanoyl-homoserine-lactone production and motility in Aliivibrio wodanis 06/09/139

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