Effect of temperature and arsenic on Aeromonas hydrophila growth, a modelling approach

Goswami, Ramansu; Mandal, Sukumar; Mandal, Sandip; Padhy, Pratap Kumar; Ray, Santanu; Mazumder, Shibnath

doi:10.2478/s11756-014-0392-6

Cited by 8 publications

(3 citation statements)

References 43 publications

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“…For example, the growth of bacterial isolates from soil and water from the Hazaribagh tannery industrial area in Bangladesh decreased with increasing concentrations of As (Prosun et al 2020). Goswami et al (2014) modeled the effect of As (arsenic trioxide, As 2 O 3 ) on the growth of Aeromonas hydrophila and observed no significant change in bacterial growth within the range of 0-30.32 μM As.…”

Section: Resultsmentioning

confidence: 99%

Cultivating efficiency: High-throughput growth analysis of anaerobic bacteria in compact microplate readers

Snoeyenbos-West,

Guerrero,

Valencia

et al. 2023

Preprint

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Anaerobic microbes play crucial roles in environmental processes, industry, and human health. Traditional methods for monitoring the growth of anaerobes, including plate counts or subsampling broth cultures for optical density measurements, are time and resource intensive. The advent of microplate readers revolutionized bacterial growth studies by enabling high-throughput and real-time monitoring of microbial growth kinetics but their use in anaerobic microbiology has remained limited. Here, we present a workflow for using small-footprint microplate readers and the Growthcurver R package to analyze the kinetic growth metrics of anaerobic bacteria. We benchmarked the small-footprint Cerillo Stratus microplate reader against a BioTek Synergy HTX microplate reader in aerobic conditions usingEscherichia coliDSM 28618 cultures. The growth rates and carrying capacities obtained from the two readers were statistically indistinguishable. However, the area under the logistic curve was significantly higher in cultures monitored by the Stratus reader. We used the Stratus to quantify the growth responses of anaerobically grownE. coliandClostridium bolteaeDSM 29485 to different doses of the toxin sodium arsenite. The growth ofE. coliandC. bolteaewas sensitive to arsenite doses of 1.3 μM and 0.4 μM, respectively. Complete inhibition of growth was achieved at 38 μM arsenite forC. bolteae, and 338 μM inE. coli. These results show that the Stratus performs similarly to a leading brand of microplate reader and can be reliably used in anaerobic conditions. We discuss the advantages of the small format microplate readers and our experiences with the Stratus.Importance statementWe present a workflow that facilitates the production and analysis of growth curves for anaerobic microbes using small-footprint microplate readers and an R script. This workflow is a cost and space-effective solution to most high-throughput solutions for collecting growth data from anaerobic microbes. This technology can be used for applications in which high-throughput would advance discovery, including microbial isolation, bioprospecting, co-culturing, host-microbe interactions, and drug/toxin-microbial interactions.

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Section: Resultsmentioning

confidence: 99%

Cultivating efficiency: High-throughput growth analysis of anaerobic bacteria in compact microplate readers

Snoeyenbos-West,

Guerrero,

Valencia

et al. 2023

Preprint

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“…The temperature stability of the crude Actinomycetes extracts were incubated in a water bath for 24 h at 4 °C, 25 °C, 35 °C, 45 °C, 55 °C, and 65 °C. The post-treatment extracts were tested to determine their ability to inhibit and destroy biofilms [28][29][30]66 . For biofilm inhibition test, 100 µL of crude extracts and 100 µL of bacterial cultures (OD 600 = 0.132) were transferred into 96-well microtiter plates then incubated at pathogens' respective temperatures for 24 h. Meanwhile, for biofilm destruction test, 100 µL of bacterial culture were transferred into 96-well microtiter plates then incubated at pathogens' respective temperature.…”

Section: Methodsmentioning

confidence: 99%

Antibiofilm properties of bioactive compounds from Actinomycetes against foodborne and fish pathogens

Miller

Waturangi²,

Yogiara³

2022

Sci Rep

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In nature, bacteria can form biofilms, multi-layered structures that adhere microbial populations to solid surfaces by exopolysaccharides, proteins, and nucleic acids. In addition to causing foodborne infections, biofilms can be a major problem in aquaculture. Actinomycetes extracts have previously demonstrated antibiofilm activity against multiple foodborne and fish pathogens, and further characterization of these extracts is needed. In this study, we identified the chemical structures and antibiofilm properties of four extracts and determined the genetic similarity of the isolates to known Streptomyces isolates. We found that several extracts contained multiple antibiofilm compounds, and the antibiofilm activities of all extracts were most stable at pH 6. Furthermore, the antibiofilm inhibition and destruction activities of the isolates were stable at different temperatures. All of crude extracts demonstrated activity against biofilms formed by foodborne and fish pathogens on the surface of stainless-steel coupons as well as polystyrene that commonly used in industrial equipment. Using PCR 16S-rRNA gene and DNA sequencing analysis, the four Actinomycetes isolates were found to be 99% (1 AC), 97% (20 PM), 95% (16 PM), and 85% (18 PM) similar to Streptomyces. Biofilm structure were analyzed using Scanning Electron Microscopy coupled with Energy-Dispersive Spectrometry analysis. Coniine/(S)-2-propylpiperidine was the most active fraction of the crude extracts of the 1 AC, 20 PM, and 16 PM isolates, and piperidine, 2-(tetrahydro-2-furanyl) was most active in the 18 PM isolate.

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“…First sampling was done at 2-3 min after the primary inoculation and was carried out at every 12 h interval throughout the experimental period for optical density measurement, taken at 688 nm wavelength for visible light 17 . Measuring the optical density of a growing culture is a common method to estimate growth of the species at specific time intervals because we know that the optical density of a growing culture increases proportionately with the increased concentration of liquid culture [17][18][19] .…”

Section: Experimental Designmentioning

confidence: 99%

Growth Profile of <i>Chaetoceros</i> Sp. and its Steady State Behaviour with Change in Initial Inoculum Size:A Modelling Approach

Kundu

Mukherjee²,

Yeasmin

et al. 2018

Current Science

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Monitoring and modelling of the growth profile of microalgae species should be an important tool for the hatchery industries before standardizing the best yielding and cost-effective protocol for their unit. Several factors are responsible in determining the nature of the growth profile. The most important regulator of such growth profile should be the volume of the initial inoculum. In addition, identification and determination of different phases (lag, log, stationary, etc.) of the growth curves of microalgae may be an essential part in the growth profile monitoring. Estimation of growth phases will also help the hatchery scientists in standardizing the commercial culture for industry. Moreover, the transition of different phases can be accurately identified through theoretical models, which are mostly overlooked in simple analysis. Summing up, we have two precise objectives: (1) to study the effects of choice of initial inocula levels on the time to maturity of the Chaetoceros sp., (2) to model the growth profile of the species from which we can theoretically determine its different phases, based on the optical density measurement as a proxy of the biomass. The estimated values of each phase are compared under two initial inocula levels through statistical tests. Using the conceptual approach of the proposed theoretical technique, there is scope for developing a similar model, which can be used in determining cost-effective culture protocol for commercial use.

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Effect of temperature and arsenic on Aeromonas hydrophila growth, a modelling approach

Cited by 8 publications

References 43 publications

Cultivating efficiency: High-throughput growth analysis of anaerobic bacteria in compact microplate readers

Cultivating efficiency: High-throughput growth analysis of anaerobic bacteria in compact microplate readers

Antibiofilm properties of bioactive compounds from Actinomycetes against foodborne and fish pathogens

Growth Profile of <i>Chaetoceros</i> Sp. and its Steady State Behaviour with Change in Initial Inoculum Size:A Modelling Approach

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