Effect of temperature acclimation on the liver antioxidant defence system of the Antarctic nototheniids Notothenia coriiceps and Notothenia rossii

Machado, Cíntia; Zaleski, Tânia; Rodrigues, Edson; Carvalho, Cleoni dos Santos; Cadena, Sílvia Maria Suter Correia; Gozzi, Gustavo Jabor; Krebsbach, Priscila; Rios, Flávia Sant’Anna; Donatti, Lucélia

doi:10.1016/j.cbpb.2014.02.003

Cited by 58 publications

(27 citation statements)

References 66 publications

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“…Similar to the findings in our study, Mueller and colleagues reported that SOD and CAT activity in the red-blooded notothenioid Notothenia coriiceps appeared relatively temperature insensitive (Mueller et al, 2012). Machado and colleagues also determined that exposure to elevated temperature in N. coriiceps and N. rossii did not elicit any changes in SOD or CAT activities (Machado et al, 2014). Furthermore, similar thermal responses have also been observed in non-notothenioid species with respect to SOD and CAT activity, with heart and liver tissues from cold-acclimated Fundulus heteroclitus and Lepomis machrochirus showing no increase in antioxidant activity levels (Grim et al, 2010).…”

Section: Tissue-specific Changes In Antioxidant Capacitysupporting

confidence: 89%

“…While these studies provide evidence that Antarctic fish are capable of acclimating to an increase in temperature, there is little information about the cost of acclimation on a cellular level. Of the studies that have examined the effects of temperature on oxidative damage in Antarctic fish, most have focused primarily on the hemoglobinless fish from the family Channicthyidae, with only three red-blooded notothens represented, Gobionotothen gibberifrons, Notothenia rossii and Notothenia coriiceps (Beers and Sidell, 2011;Mueller et al, 2011;Mueller et al, 2012;Machado et al, 2014). Moreover, with the exception of the study by Machado et al (Machado et al, 2014), which examined liver tissues of fishes acclimated up to 6 days, these studies utilized isolated mitochondria from fish exposed to their critical thermal maximum for 2 h. To date, little information exists with respect to tissue-specific levels of cellular damage in redblooded notothenioids, especially when considering potential differences in chronic versus acute exposures.…”

Section: Introductionmentioning

confidence: 99%

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Is warmer better? Decreased oxidative damage in notothenioid fish after long-term acclimation to multiple stressors

Enzor

Place

2014

Journal of Experimental Biology

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Antarctic fish of the suborder Notothenioidei have evolved several unique adaptations to deal with subzero temperatures. However, these adaptations may come with physiological trade-offs, such as an increased susceptibility to oxidative damage. As such, the expected environmental perturbations brought on by global climate change have the potential to significantly increase the level of oxidative stress and cellular damage in these endemic fish. Previous single stressor studies of the notothenioids have shown they possess the capacity to acclimate to increased temperatures, but the cellularlevel effects remain largely unknown. Additionally, there is little information on the ability of Antarctic fish to respond to ecologically relevant environmental changes where multiple variables change concomitantly. We have examined the potential synergistic effects that increased temperature and Ṗ CO2 have on the level of protein damage in Trematomus bernacchii, Pagothenia borchgrevinki and Trematomus newnesi, and combined these measurements with changes in total enzymatic activity of catalase (CAT) and superoxide dismutase (SOD) in order to gauge tissue-specific changes in antioxidant capacity. Our findings indicate that total SOD and CAT activity levels displayed only small changes across treatments and tissues. Short-term acclimation to decreased seawater pH and increased temperature resulted in significant increases in oxidative damage. Surprisingly, despite no significant change in antioxidant capacity, cellular damage returned to near-basal levels, and significantly decreased in T. bernacchii, after long-term acclimation. Overall, these data suggest that notothenioid fish currently maintain the antioxidant capacity necessary to offset predicted future ocean conditions, but it remains unclear whether this capacity comes with physiological trade-offs.

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Section: Tissue-specific Changes In Antioxidant Capacitysupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Is warmer better? Decreased oxidative damage in notothenioid fish after long-term acclimation to multiple stressors

Enzor

Place

2014

Journal of Experimental Biology

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“…Instead, the biochemical responses of biotransformation found in this study are in accordance with the previous investigations of thermal stress by other authors. These studies reported that the levels of GSH-dependent antioxidant enzymes, such as glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione S-transferase (GST), in the different tissues of Carassius auratus (at 3 and 23°C) (Bagnyukova et al, 2007), Morone saxatilis (at 7 and 25°C) (Grim et al, 2013) and Notothenia coriiceps (at 0 and 8°C) (Machado et al, 2014) were slightly affected by the increase in temperature.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, there are several reports concerning patterns of CAT expression under oxidative-stress-inducing conditions, such as chemical toxicity and thermal stress. These antioxidant defenses may be increased (Khessiba et al, 2005;Vinagre et al, 2012Vinagre et al, , 2014, inhibited (Kaur et al, 2011;Sabatini et al, 2011) or unaffected (Mueller et al, 2012;Gabriel et al, 2013;Machado et al, 2014) by agent stressors. The occurrence of one type of response or another depends on the intensity and duration of the stress applied, the susceptibility of the species that are exposed and/or the route of exposure (Bebianno et al, 2005;Sanchez et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Effects of manganese on fat snook Centropomus parallelus (Carangaria: Centropomidae) exposed to different temperatures

et al. 2017

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This study evaluates the effects of exposure to manganese (Mn 2+ ) for 96 hours at two different temperatures (24 and 27°C) on juveniles of Centropomus parallelus through the activities of glutathione S-transferase (GST) and catalase (CAT), micronuclei test (MN) and comet assay. The GST activity did not show any significant difference between the groups exposed to Mn 2+ and the respective control groups; in contrast, a major increase in the CAT activity was observed at 27°C in the group exposed to Mn 2+ compared to the control group. The genotoxic analyses showed that in all animals exposed to Mn 2+, the number of red cells with micronuclei increased significantly compared to the respective control groups. There was also a significant increase in the incidence of DNA damage in the groups exposed to Mn 2+. At a temperature of 24ºC, animals exposed to Mn 2+ had more DNA damage than those at 27°C. It is likely that the increase in temperature can also induce oxidative stress. Thus, we conclude that manganese is toxic to the fat snook juveniles, causing genotoxic damage, and when associated with an increase in temperature, manganese can also provoke an increase in oxidative stress.Keywords: Biomarkers, DNA damage, Enzymes, Genotoxicity, Metal, Micronuclei.Este estudo avaliou os efeitos da exposição ao manganês (Mn 2+ ), após 96 horas, a duas temperaturas (24 e 27°C) em juvenis de Centropomus parallelus por meio de análises bioquímicas (atividade das enzimas glutationa S-transferase (GST) e catalase (CAT)) e genotóxicas (teste do micronúcleo e ensaio cometa). A atividade da GST não mostrou diferença significativa entre os grupos expostos ao Mn 2+ e os seus respectivos grupos controle, enquanto que um aumento significativo na atividade da CAT foi observado a 27°C no grupo exposto ao Mn 2+ , quando comparado ao grupo controle. As análises genotóxicas mostraram que os animais expostos ao Mn 2+ tiveram aumento significativo na quantidade de células com micronúcleo em relação aos seus grupos de controles. Houve também aumento significativo na incidência de danos ao DNA nos grupos expostos a esse contaminante. Na temperatura de 24°C, os animais expostos ao Mn 2+ tiveram maior quantidade de danos no DNA em relação a 27°C. É provável que o aumento da temperatura também possa induzir o estresse oxidativo. Assim, concluímos que o manganês é tóxico para os juvenis de robalo, causando dano genotóxico, e quando associado a um aumento da temperatura, também pode provocar um aumento no estresse oxidativo.

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“…In particular, changes in water temperature not only cause oxidative stress in the fish body but also have negative effects on physiological phenomena, such as antioxidant control ability, immunity, and sexual maturity (Machado et al 2014;.…”

Section: Introductionmentioning

confidence: 99%

Effects of Various Light Spectra on Physiological Stress and DNA Damage by Thermal Stress in Juvenile Rock Bream (Oplegnathus fasciatus)

Choe¹,

Shin²,

Choi³

et al. 2017

Ocean and Polar Research

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: In this study, we investigated the effects of light spectra on physiology stress and DNA damage in juvenile rock bream (Oplegnathus fasciatus) using light-emitting diodes (LEDs; green, 520 nm; red, 630 nm) at two intensities (0.25 and 0.5 W/m 2 ) with application of thermal stress (25 and 30 o C). We measured the mRNA expression of heat shock protein 70 (HSP70) and the levels of plasma cortisol, glucose, aspartate aminotransferase (AspAT), and alanine aminotransferase (AlaAT). Additionally, DNA damage was measured using comet assays. Our findings showed that HSP70 mRNA expression and plasma cortisol, glucose, AspAT, and AlaAT levels were significantly higher after exposure to high temperatures and were significantly lower after exposure to green LED light. Thus, although high water temperatures induced stress in juvenile rock bream, green LED light inhibited stress. In particular, green LED light reduced stress and DNA damage to a greater degree than other light sources.

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Effect of temperature acclimation on the liver antioxidant defence system of the Antarctic nototheniids Notothenia coriiceps and Notothenia rossii

Cited by 58 publications

References 66 publications

Is warmer better? Decreased oxidative damage in notothenioid fish after long-term acclimation to multiple stressors

Is warmer better? Decreased oxidative damage in notothenioid fish after long-term acclimation to multiple stressors

Effects of manganese on fat snook Centropomus parallelus (Carangaria: Centropomidae) exposed to different temperatures

Effects of Various Light Spectra on Physiological Stress and DNA Damage by Thermal Stress in Juvenile Rock Bream (Oplegnathus fasciatus)

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