Effect of tea tree oil on Staphylococcus aureus growth and enterotoxin production

Shi, Ce; Zhao, Xingchen; Yan, Haiyang; Meng, Rizeng; Zhang, Yan; Li, Wenli; Liu, Zonghui; Guo, Na

doi:10.1016/j.foodcont.2015.10.049

Cited by 42 publications

(31 citation statements)

References 37 publications

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“…S. aureus is also considered a foodborne pathogen, as this type of bacteria can produce enterotoxins in contaminated food. Given this, foodborne spoilages resulting from S. aureus infections present a major food safety challenge (Abdolshahi et al, ; Shi et al, ). It was reported that throughout the logarithmic and stationary phases of its growth, pathogenic S. aureus secretes virulence factors such as hemolysin, staphylococcal enterotoxins (SEs), toxic shock syndrome toxin (TSST), and other factors (Li et al, ; Shi et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Given this, foodborne spoilages resulting from S. aureus infections present a major food safety challenge (Abdolshahi et al, ; Shi et al, ). It was reported that throughout the logarithmic and stationary phases of its growth, pathogenic S. aureus secretes virulence factors such as hemolysin, staphylococcal enterotoxins (SEs), toxic shock syndrome toxin (TSST), and other factors (Li et al, ; Shi et al, ). Virulent S. aureus generally produces coagulase, lecithinase, DNase, and hemolysin, which have been identified as indicators of pathogenicity (Lee, Kim, Park, & Lee, ).…”

Section: Introductionmentioning

confidence: 99%

“…Particularly, staphylococcal hemolysin possesses hemolytic, cytolytic, and dermonecrotic activities against human erythrocytes, monocytes, lymphocytes, macrophages, and epithelial cells (Jing et al, ; Qiu et al, ). Additionally, 20 different types of enterotoxins have been discovered to arise from S. aureus , and enterotoxin types A–E are the most common enterotoxins that cause food poisoning in humans (Shi et al, ). Qiu et al () reported that these enterotoxins exhibit the immunomodulatory properties of super antigens and can stimulate T‐cell activation and cytokine release from T‐cells.…”

Section: Introductionmentioning

confidence: 99%

“…phases of its growth, pathogenic S. aureus secretes virulence factors such as hemolysin, staphylococcal enterotoxins (SEs), toxic shock syndrome toxin (TSST), and other factors (Li et al, 2018;Shi et al, 2016). Virulent S. aureus generally produces coagulase, lecithinase, DNase, and hemolysin, which have been identified as indicators of pathogenicity (Lee, Kim, Park, & Lee, 2017).…”

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confidence: 99%

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Inhibitory effects of Inula britannica extract fermented by Lactobacillus plantarum KCCM 11613P on coagulase activity and growth of Staphylococcus aureus including methicillin‐resistant strains

Bae

Kim

et al. 2019

J Food Biochem

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The aim of this study was to evaluate the antimicrobial efficacy of fermented Inula britannica extract (FIBE) against Staphylococcus aureus strains including methicillin‐resistant S. aureus (MRSA). I. britannica extract was fermented by Lactobacillus plantarum KCCM 11613P, and the pathogenicity of S. aureus strains was determined via assessment of coagulase, DNase, and hemolytic activities. Epicatechin concentration increased from 4.38 to 6.05 μg/mg during fermentation (p < 0.01). FIBE treatment inhibited coagulase release from S. aureus to levels below the inhibitory concentration. FIBE promoted the release of intracellular nucleic acids and N‐phenyl‐1‐naphthylamine absorption. In three S. aureus strains, damaged cells exhibited 21.58, 16.79, and 17.65% decreases in membrane potential induced by cell membrane depolarization, respectively (p < 0.05). Upon FIBE treatment in culture, the minimum inhibitory concentration of FIBE exerted a bacteriostatic effect. In conclusion, FIBE possesses antimicrobial properties, including inhibition of virulence factors, damage to cell membranes, and inhibition of bacterial growth. Practical applications Methicillin‐resistant Staphylococcus aureus (MRSA) is a serious concern in hospitals because of its known antibiotic resistance. Vancomycin and tigecycline are used for treating MRSA, but the appearance of vancomycin‐intermediate and multidrug‐resistant strains of these bacteria has created a demand for new antimicrobial agents. This study demonstrates the effective application of Inula britannica and fermentation technology for developing natural antimicrobial agents against methicillin‐resistant Staphylococcus aureus.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Inhibitory effects of Inula britannica extract fermented by Lactobacillus plantarum KCCM 11613P on coagulase activity and growth of Staphylococcus aureus including methicillin‐resistant strains

Bae

Kim

et al. 2019

J Food Biochem

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“…Because of its bioactivities, its efficacy has recently been evaluated against Staphylococcus aureus, Escherichia coli, Aspergillus niger , and Candida albicans in vitro (Gustafson et al, 1998; Hammer et al, 2003; Shi et al, 2016). TTO controls the growth of B. cinerea more effectively than pine and cinnamon oil (Szczerbanik et al, 2007), or clove oil and garlic oil (Cheng and Shao, 2011).…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Analysis and Mode of Action of Metabolites of Tea Tree Oil Involved in the Suppression of Botrytis cinerea

Shao

et al. 2017

Front. Microbiol.

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Tea tree oil (TTO), a volatile essential oil, has been widely used as an antimicrobial agent. However, the mechanism underlying TTO antifungal activity is not fully understood. In this study, a comprehensive metabolomics survey was undertaken to identify changes in metabolite production in Botrytis cinerea cells treated with TTO. Significant differences in 91 metabolites were observed, including 8 upregulated and 83 downregulated metabolites in TTO-treated cells. The results indicate that TTO inhibits primary metabolic pathways through the suppression of the tricarboxylic acid (TCA) cycle and fatty acid metabolism. Further experiments show that TTO treatment decreases the activities of key enzymes in the TCA cycle and increases the level of hydrogen peroxide (H2O2). Membrane damage is also induced by TTO treatment. We hypothesize that the effect of TTO on B. cinerea is achieved mainly by disruption of the TCA cycle and fatty acid metabolism, resulting in mitochondrial dysfunction and oxidative stress.

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The dynamics and mechanism of the antimicrobial activity of tea tree oil against bacteria and fungi

Shi

et al. 2016

Appl Microbiol Biotechnol

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Tea tree oil (TTO) is a yellow liquid extracted from Melaleuca alternifolia. Although the antimicrobial activity of TTO has been known for a long time, its specific antimicrobial effects and mechanism underlying these remain poorly characterized. The present study investigated the chemical composition of TTO and the dynamics and mechanism of its antimicrobial activities in two bacterial and two fungal strains. Gas chromatography-mass spectrometry analysis identified alkenes and alcohols as the main constituents of TTO. Terpinen-4-ol was the most abundant individual component, accounting for approximately 23 % of the TTO. Poisoned food technique assessment showed that the minimum inhibitory concentrations of TTO for bacterial strains (Escherichia coli and Staphylococcus aureus) and fungal strains (Candida albicans and Aspergillus niger) were 1.08 and 2.17 mg/mL, respectively. Antimicrobial dynamic curves showed that with increasing concentrations of TTO, the rate of cell killing and the duration of growth lag phase increased correspondingly. These data indicated that TTO produced concentration and time-dependent antimicrobial effects. The minimum bactericidal and fungicidal concentrations of TTO were 2.17, 4.34, and 4.34 against E. coli, S. aureus, and C. albicans, respectively. However, A. niger conidia were not completely eradicated, even after 3 days in the presence of 17.34 mg/mL TTO. Transmission electron microscopy images indicated that TTO penetrated the cell wall and cytoplasmic membrane of all the tested bacterial and fungal strains. TTO may also penetrate fungal organelle membrane. These findings indicated that TTO maybe exerts its antimicrobial effects by compromising the cell membrane, resulting in loss of the cytoplasm and organelle damage, which ultimate leads to cell death.

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Effect of tea tree oil on Staphylococcus aureus growth and enterotoxin production

Cited by 42 publications

References 37 publications

Inhibitory effects of Inula britannica extract fermented by Lactobacillus plantarum KCCM 11613P on coagulase activity and growth of Staphylococcus aureus including methicillin‐resistant strains

Inhibitory effects of Inula britannica extract fermented by Lactobacillus plantarum KCCM 11613P on coagulase activity and growth of Staphylococcus aureus including methicillin‐resistant strains

Metabolomic Analysis and Mode of Action of Metabolites of Tea Tree Oil Involved in the Suppression of Botrytis cinerea

The dynamics and mechanism of the antimicrobial activity of tea tree oil against bacteria and fungi

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