Effect of TDZ on Various Plant Cultures

Pai, Sandeep R.; Desai, Neetin

doi:10.1007/978-981-10-8004-3_25

Cited by 16 publications

(15 citation statements)

References 80 publications

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“…Thidiazuron (TDZ) (N-phenyl-N′-1,2,3-thiadiazol-5-ylurea) is a thiadiazole-substituted phenylurea, which was reported to possess more cytokinin activity as compared to phenyl urea derivatives and other active adenine types of cytokinins [34]. Thidiazuron was also reported to possess actions of other plant growth regulators such as auxins, gibberellins, and ethylene [35]. Thidiazuron (TDZ) was used by several researchers in regeneration studies of medicinal plants with higher frequency of shoot let production from friable callus [36, 37].…”

Section: Resultsmentioning

confidence: 99%

Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

Rohela

Jogam

Bylla

et al. 2019

BioMed Research International

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Rauwolfia tetraphylla L. is an important medicinal plant species which is well known for its pharmaceutically important alkaloids. In the present study, we are reporting about its conservation by in vitro clonal multiplication through the standardized protocol of indirect regeneration by using leaf and stem based callus and assessment of genetic fidelity of acclimated plantlets by start codon targeted (SCoT), inter simple sequence repeats (ISSR), and randomly amplified polymorphic DNA (RAPD) marker based analysis. Initially friable callus was induced in maximum amounts (378.7, 323.8, and 412.8 in mg) from leaf, root, and stem explants on Murashige and Skoog (MS) media supplemented with 5.0 mg/L, 3.0 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D) and 5.0 mg/L of naphthalene acetic acid (NAA), respectively. Shoot regeneration with the maximum number of shoot buds (25 and 20) was obtained from leaf and stem calluses on MS media supplemented with TDZ (0.25 mg/L) + BAP (2 mg/L). The regenerated shoots were rooted successfully with maximum rooting percentage of 98.0 on full strength MS media amended with IAA (1.0 mg/L) and IBA (1.0 mg/L). The regenerated plantlets were hardened using 2:1 ratio of sterile garden soil and sand, followed by acclimatization in field conditions with 86% of survival. SCoT, ISSR, and RAPD primers based polymerase chain reaction (PCR) analysis was carried out to check possible genetic variations in micro propagated plants in comparison with mother plant. Among the ten SCoT (S), ISSR (R), and RAPD (OPA) primers used, S2, R10, and OPA3 has given good amplification with scorable DNA bands. The results revealed that the regenerated plants did not have any polymorphism with mother plant. Hence, the in vitro regenerated R. tetraphylla plantlets were confirmed as true-to-type.

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Section: Resultsmentioning

confidence: 99%

Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

Rohela

Jogam

Bylla

et al. 2019

BioMed Research International

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“…The data of bud emergence can be seen at Remarks: Rep.=replication, consist of 1 bottle with 6-8 explants each; dap= days after planting; the punctuation mark of " -" means no bud was detected from all explants. (Pai and Desai, 2018). It has cytokinin activity and has been reported to be effective in stimulating adventitious shoot formation and promoting axillary shoot proliferation in micropropagation (Lu, 1993) and also reported as a potent cytokinin for woody and recalcitrant species in tissue culture (Huetteman & Preece, 1993).…”

Section: Resultsmentioning

confidence: 99%

RUNNER-TIP CULTURE OF STRAWBERRY (Fragaria x ananassa Duch) GROWN ON SEVERAL SHOOT-INDUCTION MEDIUM

Dwiyani

Yuswanti

Mayadewi

et al. 2020

Int. J. Biosci. Biotech.

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A research regarding “Runner-tip culture of strawberry (Fragaria x ananassa Duch) Grown on Several Shoot-induction Medium” has been investigated. The objective of the research was to find out the most suitable medium for shoot production from runner-tip culture of strawberry at establishment step of micropropagation. The research was laid out in a Completely Randomized Design, 4 treatments of medium type for shoot induction and 10 replication, each was represented by one (1) bottle with 6-8 explants. The treatments were summarized as follows: T1 = MS ; T2 = MS + 2 ppm BAP + 0.01 ppm NAA; T3 = MS + 1 ppm of TDZ; T4 = WPM + 2 ppm BAP + 0.01 ppm NAA. The parameters observed were days of the bud emergence, the average number of shoots per explant, and the average number of leaves per explant. It can be concluded that among medium used in the current research, the medium of MS added with 1 ppm thidiazuron (TDZ) was the most suitable medium for shoot production of strawberry from the explant of runner-tips. The treatment was resulted in the earliest time of bud emergence, and producing the highest number of shoots and leaves.

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“…Plant Growth Regulators (PGRs) are non-nutrient organic molecules. NAA, TIBA, IAA, GA3, Cytokinin, and other synthetic PGR-like substances are sold commercially [6]. Plant Growth regulators have been used for a wide range of purposes, including overcoming dormancy problems in seeds, accelerating seedling growth, hastening and increasing rooting of cuttings in several vegetative propagated plants, and altering plant growth in areas where genetic or other manipulation is not possible.…”

Section: Introductionmentioning

confidence: 99%

Effect of Plant Growth Regulators on the Morphology, Flowering, Maturity, Yield Attributes, and Yield of Lentil (Lens culinaris L. Medik.)

Prasad

Tripathi

Rawat

et al. 2023

IJECC

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Comprehensively, nutrient deficiencies in humans and animals are a quiet epidemic in many underdeveloped nations. Nutrient deficiencies in humans and animals are a global problem in most developing countries. Lentil is one of the most important and nutritious Rabi pulse in India. It’s a leguminous crop that improves the soil fertility through biological nitrogen fixation. In this contest an experiment was conducted to study the enhancement of plant growth, chlorophyll content, flowering, yield and seed protein content by plant growth regulators in DPL-62 lentil (Lens culinaris L. Medik) variety during two Rabi seasons of 2019-20 and 2020-21. The field experiment was laid out in randomized block design (RBD). Five growth regulators viz. triiodobenzoic acid (TIBA), gibberellic acid (GA3), cytokinin, indole acetic acid (IAA), and naphthaleneacetic acid (NAA) were used in Eleven treatments were tested T1: Control, T2: 25 ppm TIBA, T3: 50 ppm TIBA, T4: 25 ppm GA3, T5: 50 ppm GA3, T6: 5 ppm Cytokinin, T7: 10 ppm Cytokinin, T8: 25 ppm IAA, T9: 50 ppm IAA, T10: 25 ppm NAA, T11: 50 ppm NAA. The results revealed that the maximum plant height (38.12 & 38.15 cm at harvest) was obtained by the 50 ppm GA3 followed by 50 ppm IAA (37.00 & 38.10 cm at harvest) spray at all stages. However, the 50 ppm TIBA showed better results (per plant) viz., number of branches (15.00, 15.33 at 110DAS), leaf area (86.50, 86.32 at 110DAS), number of pods (160.20, 160.37), pod setting (65.45, 65.50%), seed weight (2.02, 2.10g), test weight (27.32, 27.39g) and seed yield (1500.00, 1510.00 kg ha-1) of lentil as compared to all other treatments. The maximum days of flowering (67.50, 67.85) and days of maturity. On the basis of the observed results the growing of DPL-60 lentil variety with foliar applications of 50 ppm TIBA is recommended for the farmers of the test area for greater production and profitability.

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Effect of TDZ on Various Plant Cultures

Cited by 16 publications

References 80 publications

Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

RUNNER-TIP CULTURE OF STRAWBERRY (Fragaria x ananassa Duch) GROWN ON SEVERAL SHOOT-INDUCTION MEDIUM

Effect of Plant Growth Regulators on the Morphology, Flowering, Maturity, Yield Attributes, and Yield of Lentil (Lens culinaris L. Medik.)

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