Effect of Systemic and Orally Administered Iota-Carrageenan on Ovalbumin-Specific Antibody Response in the Rat

Coste, Michel; Dubuquoy, Catherine; Tomé, Daniel

doi:10.1159/000234735

Cited by 7 publications

(5 citation statements)

References 9 publications

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“…Carrageenan is used as a component to induce non-specific inflammation, possibly through activation of innate immune responses. It modulates acquired immunity, adjuvant and suppressive effects (Coste et al, 1989;Macino and Morelli, 1983;Bash and Vago, 1980;Nicklin and Miller, 1984;Cochran and Baxter, 1984;Vijayakumar et al, 1990). In our results, we observed that CAR stimulated-macrophages displayed a remarkable increase (142-75%) of nitric oxide production as compared to nonstimulated cells and a significant increase of about 50-33% as compared to the macrophages collected from mice stimulated with LPS.…”

Section: Discussionsupporting

confidence: 57%

Morphological and Biochemical Characterization of Macrophages Activated by Carrageenan and Lipopolysaccharide In Vivo

Nacife

Soeiro

Gomes

et al. 2004

Cell Struct. Funct.

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ABSTRACT. Macrophages are able to recognize, internalize and destroy a large number of pathogens, thus restricting the infection until adaptive immunity is initiated. In this work our aim was to analyze the surface charge of cells activated by carrageenan (CAR) and lipopolysaccharide (LPS) through light and electron microscopy approaches as well as the release of inflammatory mediators in vitro. The ultrastuctural analysis and the light microscopy data showed that in vivo administration of CAR represents a potent inflammatory stimulation for macrophages leading to a high degree of spreading, an increase in their size, in the number of the intracellular vacuoles and membrane projections as compared to the macrophages collected from untreated animals as well as mice submitted to LPS. Our data demonstrated that CAR stimulated-macrophages displayed a remarkable increase in nitric oxide production and PGE2 release as compared to the cells collected from non-stimulated and stimulated mice with LPS in vivo. On the other hand, non-stimulated macrophages as well as macrophages stimulated by LPS produce almost the same quantities of TNF-α, while in vivo stimulation by CAR leads to a 30-40% increase of cytokine release in vitro compared to the other groups. In conclusion, our morphological and biochemical data clearly showed that in vivo stimulation with CAR induces a potent inflammatory response in macrophages representing an interesting model to analyze inflammatory responses.

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Section: Discussionsupporting

confidence: 57%

Morphological and Biochemical Characterization of Macrophages Activated by Carrageenan and Lipopolysaccharide In Vivo

Nacife

Soeiro

Gomes

et al. 2004

Cell Struct. Funct.

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“…Carrageenan is widely used by the food industry due to its gelling and stabilizing properties, and it has been extensively used as a reagent to induce non‐specific inflammation, possibly through activation of innate immune responses. Carrageenan has also been ascribed the property of modulating acquired immunity, and both adjuvant and suppressive effects have been reported [2–7]. Such inconsistent outcomes may be due to differences in the route of administration, dosage and heterogeneity of carrageenan preparations.…”

Section: Discussionmentioning

confidence: 99%

“…Several reports describe the modulation of acquired immunity by carrageenan, where both adjuvant and suppressive effects are observed. Adjuvant effects are usually obtained by systemic administration of carrageenan, resulting in augmentation of antibody responses [2, 3]. On the contrary, several studies demonstrate that both systemic and oral administration of carrageenans suppress antibody production and mitogen‐induced T cell proliferation [4–7].…”

Section: Introductionmentioning

confidence: 99%

Suppression of allergic reaction by λ‐carrageenan: Toll‐like receptor 4/MyD88‐dependent and ‐independent modulation of immunity

Tsuji

Hoshino

Noro

et al. 2003

Clin Experimental Allergy

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“…Previous studies reported similarly diminished lymphocyte responsiveness in presence of carrageenan, without a visible modification, or a toxic effect on intraepithelial lymphocytes or lymph node T cells (3,7,13). To explain low lymphocyte responsiveness, several authors implicated a role for macrophages (8,14).…”

Section: Discussionmentioning

confidence: 68%

“…Although large amounts of oral carrageenan induce colitis (5,6), small quantities do not appear to be toxic for the gut mucosa (3). Studies in rats have demonstrated an adjuvant effect similar to alum when carrageenan was given with ovalbumin by systemic route, but abrogation of the PCA response when given orally (7). Diminished cellular immune response mediated by carrageenan has also been demonstrated in other models.…”

mentioning

confidence: 96%

Oral Carrageenan Induces Antigen-Dependent Oral Tolerance: Prevention of Anaphylaxis and Induction of Lymphocyte Anergy in a Murine Model of Food Allergy

2001

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Immunosuppressive effects of carrageenan, a high-molecularweight polysaccharide, on antibody and T cell responses have been previously demonstrated. However, its effect on anaphylaxis is unknown. Our objectives were to test carrageenanmediated oral tolerance induction in young mice subsequently sensitized to a common cow's milk antigen. C3H/HeJ mice were fed or not -carrageenan (0.5 g/L) and/or 0.01 mg/mL ␤-lactoglobulin (BLG) for 5 d before oral sensitization with BLG and cholera toxin. Subsequently, the mice were challenged with BLG and symptom scores of anaphylaxis were recorded. Mesenteric lymph node cells, spleen cells, Peyer's patches cells, intraepithelial lymphocytes, and lamina propria lymphocytes were isolated and stimulated in vitro with BLG, IL-2, or left unstimulated. BLG-specific IgG, IgG 1 , and IgG 2a antibodies were measured. Pretreatment with carrageenan and BLG, but not pretreatment with either carrageenan or BLG alone or omission of pretreatment, diminished significantly the number of anaphylactic mice after BLG challenge (6.3% versus 53% in mice without pretreatment, p ϭ 0.006). Mesenteric lymph nodes and spleen cells from pretreated mice proliferated less in presence of BLG or IL-2 than cells from sensitized control mice. Antigen-specific antibody production and passive cutaneous anaphylaxis was not suppressed by carrageenan and BLG pretreatment. In conclusion, carrageenan administered to young mice in conjunction with low doses of allergen before sensitization efficiently prevents anaphylaxis. During infancy, large amounts of potentially harmful food antigens are ingested in large quantities and, in most infants, oral tolerance is naturally acquired. Animal studies have revealed that early oral exposure to low amounts of antigen tend to promote sensitization, whereas large amounts favor tolerance induction (1). In human infants it is accepted, that potential food allergens should be completely avoided during the first months of life in infants at risk for allergy. However, these measures are only partially efficient in atopic individuals (2). Development of further strategies favoring induction of earlylife antigen-specific tolerance are strongly needed.Carrageenan is a high-molecular-weight galactose-based polysaccharide. When ingested it is found mostly undegraded in the gut submucosa (3), where it is taken up and cleared by macrophages (4). Although large amounts of oral carrageenan induce colitis (5, 6), small quantities do not appear to be toxic for the gut mucosa (3). Studies in rats have demonstrated an adjuvant effect similar to alum when carrageenan was given with ovalbumin by systemic route, but abrogation of the PCA response when given orally (7). Diminished cellular immune response mediated by carrageenan has also been demonstrated in other models. Bash and Vago fed rats with carrageenan and observed a strongly diminished cellular response in spleen cells and mesenteric lymph nodes cells (8). Rumjanek and Brent showed that intraperitoneal carrageenan suppressed T cell activity ...

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Effect of Systemic and Orally Administered Iota-Carrageenan on Ovalbumin-Specific Antibody Response in the Rat

Cited by 7 publications

References 9 publications

Morphological and Biochemical Characterization of Macrophages Activated by Carrageenan and Lipopolysaccharide In Vivo

Morphological and Biochemical Characterization of Macrophages Activated by Carrageenan and Lipopolysaccharide In Vivo

Suppression of allergic reaction by λ‐carrageenan: Toll‐like receptor 4/MyD88‐dependent and ‐independent modulation of immunity

Oral Carrageenan Induces Antigen-Dependent Oral Tolerance: Prevention of Anaphylaxis and Induction of Lymphocyte Anergy in a Murine Model of Food Allergy

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