Effect of synthetic surfactants and soapwort (Saponaria officinalis L.) extract on skin-mimetic model lipid monolayers

Jurek, Ilona; Góral, Ilona; Mierzyńska, Zofia; Moniuszko-Szajwaj, Barbara; Wojciechowski, Kamil

doi:10.1016/j.bbamem.2018.12.005

Cited by 24 publications

(19 citation statements)

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“…The high resistance of the DPPC/cholesterol liposomes to the anionic surfactants is also surprising in view of our previous results on solubilization of monolayers with the same lipid composition upon contact with SLS, ALS and SLES introduced into the subphase [ 21 ]. These monolayers were completely solubilized at the surfactant concentration as low as 1%, i.e., much lower than for the bilayers.…”

Section: Resultsmentioning

confidence: 96%

“…Only for the amphoteric CAPB, the solubilization capacity is comparable towards the mono- and bilayers (both the monolayer and the liposomes vanished in presence of CAPB). In contrast, 1% SAP solution did not solubilize the same monolayer, although clear signs of incorporation in the lipid structure could be noted [ 21 ]. The same solution at 5% dry mass increased the size of the liposomes from below 100 nm to over 300 nm (note the disappearance of the original large structures, d 32 = 1178 ± 165 nm, observed in bare SAP solutions).…”

Section: Resultsmentioning

confidence: 99%

“…Because of the high affinity to membrane lipids, especially to cholesterol [ 25 , 26 ], some saponins display various levels of hemolytic activity [ 27 ]. However, we demonstrated that numerous isolated saponins and saponin-rich extracts do not have any degrading effect on lipid monolayers, as opposed to several synthetic surfactants [ 21 , 28 , 29 , 30 , 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

“…One of the plants widely distributed around the globe and abundant in biosurfactants is Saponaria officinalis L., commonly known as soapwort, a perennial herb f–rom the Caryophyllaceae family [ 19 ]. The plant produces high amounts of glycosidic compounds (saponins), capable of lowering surface tension to the extent comparable to that of synthetic surfactants [ 17 , 20 , 21 ]. For centuries, the aqueous extracts of soapwort roots have not only been used as a natural detergent but also as an emulsifier, and even as a medicine to relieve coughing [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Soapwort (Saponaria officinalis L.) Extract vs. Synthetic Surfactants—Effect on Skin-Mimetic Models

et al. 2021

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Our skin is continuously exposed to different amphiphilic substances capable of interaction with its lipids and proteins. We describe the effect of a saponin-rich soapwort extract and of four commonly employed synthetic surfactants: sodium lauryl sulfate (SLS), sodium laureth sulfate (SLES), ammonium lauryl sulfate (ALS), cocamidopropyl betaine (CAPB) on different human skin models. Two human skin cell lines were employed: normal keratinocytes (HaCaT) and human melanoma cells (A375). The liposomes consisting of a dipalmitoylphosphatidylcholine/cholesterol mixture in a molar ratio of 7:3, mimicking the cell membrane of keratinocytes and melanoma cells were employed as the second model. Using dynamic light scattering (DLS), the particle size distribution of liposomes was analyzed before and after contact with the tested (bio)surfactants. The results, supplemented by the protein solubilization tests (albumin denaturation test, zein test) and oil emulsification capacity (using olive oil and engine oil), showed that the soapwort extract affects the skin models to a clearly different extent than any of the tested synthetic surfactants. Its protein and lipid solubilizing potential are much smaller than for the three anionic surfactants (SLS, ALS, SLES). In terms of protein solubilization potential, the soapwort extract is comparable to CAPB, which, however, is much harsher to lipids.

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Soapwort (Saponaria officinalis L.) Extract vs. Synthetic Surfactants—Effect on Skin-Mimetic Models

et al. 2021

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“…Other lipid-based models have been reported as skin mimetic systems and were used for the study of the effect of a set of synthetic surfactants on the skin. These models contain dipalmitoylphosphatidylcholine (DPPC) and cholesterol or a mixture of ceramide, stearic acid, and cholesterol(Jurek, Góral, Mierzy nska, Moniuszko-Szajwaj, & Wojciechowski, 2019). Recently, our research group developed a cheaper and simpler alternative that simulates SC(Shakel, Nunes, Costa Lima, & Reis, 2019) and can allow the screening of drug candidates.…”

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confidence: 99%

Human skin models: From healthy to disease‐mimetic systems; characteristics and applications

Moniz

Lima

2020

British J Pharmacology

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Skin drug delivery is an emerging route in drug development, leading to an urgent need to understand the behaviour of active pharmaceutical ingredients within the skin. Given, As one of the body's first natural defences, the barrier properties of skin provide an obstacle to the successful outcome of any skin drug therapy. To elucidate the mechanisms underlying this barrier, reductionist strategies have designed several models with different levels of complexity, using non‐biological and biological components. Besides the detail of information and resemblance to human skin in vivo, offered by each in vitro model, the technical and economic efforts involved must also be considered when selecting the most suitable model. This review provides an outline of the commonly used skin models, including healthy and diseased conditions, in‐house developed and commercialized models, their advantages and limitations, and an overview of the new trends in skin‐engineered models.

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Engineering and Development of a Tissue Model for the Evaluation of Microneedle Penetration Ability, Drug Diffusion, Photothermal Activity, and Ultrasound Imaging: A Promising Surrogate to Ex Vivo and In Vivo Tissues

et al. 2023

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Driven by regulatory authorities and the ever‐growing demands from industry, various artificial tissue models have been developed. Nevertheless, there is no model to date that is capable of mimicking the biomechanical properties of the skin whilst exhibiting the hydrophilicity/hydrophobicity properties of the skin layers. As a proof‐of‐concept study, tissue surrogates based on gel and silicone are fabricated for the evaluation of microneedle penetration, drug diffusion, photothermal activity, and ultrasound bioimaging. The silicone layer aims to imitate the stratum corneum while the gel layer aims to mimic the water‐rich viable epidermis and dermis present in in vivo tissues. The diffusion of drugs across the tissue model is assessed, and the results reveal that the proposed tissue model shows similar behavior to a cancerous kidney. In place of typical in vitro aqueous solutions, this model can also be employed for evaluating the photoactivity of photothermal agents since the tissue model shows a similar heating profile to skin of mice when irradiated with near‐infrared laser. In addition, the designed tissue model exhibits promising results for biomedical applications in optical coherence tomography and ultrasound imaging. Such a tissue model paves the way to reduce the use of animals testing in research whilst obviating ethical concerns.

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Effect of synthetic surfactants and soapwort (Saponaria officinalis L.) extract on skin-mimetic model lipid monolayers

Cited by 24 publications

References 46 publications

Soapwort (Saponaria officinalis L.) Extract vs. Synthetic Surfactants—Effect on Skin-Mimetic Models

Soapwort (Saponaria officinalis L.) Extract vs. Synthetic Surfactants—Effect on Skin-Mimetic Models

Human skin models: From healthy to disease‐mimetic systems; characteristics and applications

Engineering and Development of a Tissue Model for the Evaluation of Microneedle Penetration Ability, Drug Diffusion, Photothermal Activity, and Ultrasound Imaging: A Promising Surrogate to Ex Vivo and In Vivo Tissues

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