Effect of synthetic and natural media on lipid production from Fusarium oxysporum

Μάτσακας, Λεωνίδας; Giannakou, Maria; Vörös, Dimitrij

doi:10.1016/j.ejbt.2017.10.003

Cited by 28 publications

(9 citation statements)

References 62 publications

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“…The FAME (fatty acid methyl ester) was collected in the n-hexane layer after centrifugation. The FAMEs were analyzed by gas chromatography (Varian CP-3800; Agilent Technologies, Santa Clara, CA, USA) using a capillary column (WCOT-fused silica 100-m × 0.25-mm coating, CPSIL 88; Agilent Technologies) under operating conditions that have previously been reported [ 63 ]. Estimation of biodiesel properties was done using the following derived empirical formulae [ 6 ]: where Mi was the molecular weight of each fatty acid, DB was the number of double bonds, FA was the fatty acid content (%), MUFA was the monounsaturated fatty acid content, and PUFA was the polyunsaturated fatty acid content.…”

Section: Methodsmentioning

confidence: 99%

Heterotrophic cultivation of Auxenochlorella protothecoides using forest biomass as a feedstock for sustainable biodiesel production

Patel

Μάτσακας

Rova

et al. 2018

Biotechnol Biofuels

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BackgroundThe aim of this work was to establish a process for the heterotrophic growth of green microalgae using forest biomass hydrolysates. To provide a carbon source for the growth of the green microalgae, two forest biomasses (Norway spruce and silver birch) were pretreated with a hybrid organosolv-steam explosion method, resulting in inhibitor-free pretreated solids with a high cellulose content of 77.9% w/w (birch) and 72% w/w (spruce). Pretreated solids were hydrolyzed using commercial cellulolytic enzymes to produce hydrolysate for the culture of algae.ResultsThe heterotrophic growth of A. protothecoides was assessed using synthetic medium with glucose as carbon source, where the effect of sugar concentration and the carbon-to-nitrogen ratio were optimized, resulting in accumulation of lipids at 5.42 ± 0.32 g/L (64.52 ± 0.53% lipid content) after 5 days of culture on glucose at 20 g/L. The use of birch and spruce hydrolysates was favorable for the growth and lipid accumulation of the algae, resulting in lipid production of 5.65 ± 0.21 g/L (66 ± 0.33% lipid content) and 5.28 ± 0.17 g/L (63.08 ± 0.71% lipid content) when grown on birch and spruce, respectively, after only 120 h of cultivation.ConclusionsTo the best of our knowledge, this is the first report of using organosolv pretreated wood biomass hydrolysates for the growth and lipid production of microalgae in the literature. The pretreatment process used in this study provided high saccharification of biomass without the presence of inhibitors. Moreover, the lipid profile of this microalga showed similar contents to vegetable oils which improve the biodiesel properties.

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Section: Methodsmentioning

confidence: 99%

Heterotrophic cultivation of Auxenochlorella protothecoides using forest biomass as a feedstock for sustainable biodiesel production

Patel

Μάτσακας

Rova

et al. 2018

Biotechnol Biofuels

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“…Unexpectedly, the biomass concentration of the engineered strain using yeast extract was less than the cultures using nitrate sources, which is in contrast with the published research works, mentioning that yeast extract was the best nitrogen source for fermentation of several filamentous fungi, such as Aspergillus sp. and Fusarium oxysporum ( Colla et al, 2016 ; Matsakas et al, 2017 ). Actually, the nitrogen source suitable for cell growth might not enhance lipid production.…”

Section: Discussionmentioning

confidence: 99%

“…such as Aspergillus sp. and Fusarium oxysporum (Colla et al, 2016;Matsakas et al, 2017). Actually, the nitrogen source suitable for cell growth might not enhance lipid production.…”

Section: Kinetic Models Parameters Valuesmentioning

confidence: 99%

Physiological Traits of Dihomo-γ-Linolenic Acid Production of the Engineered Aspergillus oryzae by Comparing Mathematical Models

et al. 2020

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Dihomo-γ-linolenic acid (DGLA; C20:3 n-6) is expected to dominate the functional ingredients market for its role in anti-inflammation and anti-proliferation. The DGLA production by the engineered strain of Aspergillus oryzae with overexpressing Pythium 6-desaturase and 6-elongase genes was investigated by manipulating the nutrient and fermentation regimes. Of the nitrogen sources tested, the maximum biomass and DGLA titers were obtained in the cultures using NaNO 3 grown at pH 6.0. For establishing economically feasible process of DGLA production, the cost-effective medium was developed by using cassava starch hydrolysate (CSH) and NaNO 3 as carbon and nitrogen sources, respectively. The supplementation with 1% (v/v) mother liquor (ML) into the CSH medium promoted the specific yield of DGLA production (Y DGLA/X) comparable with the culture grown in the defined NaNO 3 medium, and the DGLA proportion was over 22% in total fatty acid (TFA). Besides, the GLA was also generated at a similar proportion (about 25% in TFA). The mathematical models of the cultures grown in the defined NaNO 3 and CSH/ML media were generated, describing that the lipid and DGLA were growth-associated metabolites corresponding to the relevant kinetic parameters of fermentations. The controlled mode of submerged fermentation of the engineered strain was explored for governing the PUFA biosynthesis and lipid-accumulating process in relation to the biomass production. This study provides an informative perspective in the n-6 fatty acid production through physiological manipulation, thus leading to a prospect in viable production of the DGLA-enriched oil by the engineered strain.

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“…Lipomyces starkeyi CBS 1807 Sweet sorghum stalks juice 30 [13] Fungi Fusarium oxysporum Sweet sorghum stalks (12% w/w solid load) 22 [14] Glucose 42…”

Section: Rhodosporidium Toruloidesmentioning

confidence: 99%

Lipids detection and quantification in oleaginous microorganisms: an overview of the current state of the art

2019

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Oleaginous microorganisms are among the most promising feedstocks for the production of lipids for biofuels and oleochemicals. Lipids are synthesized in intracellular compartments in the form of lipid droplets. Therefore, their qualitative and quantitative analysis requires an initial pretreatment step that allows their extraction. Lipid extraction techniques vary with the type of microorganism but, in general, the presence of an outer membrane or cell wall limits their recovery. This review discusses the various types of oleaginous microorganisms, their lipid accumulating capabilities, lipid extraction techniques, and the pretreatment of cellular biomass for enhanced lipid recovery. Conventional methods for lipid quantification include gravimetric and chromatographic approaches; whereas nonconventional methods are based on infrared, Raman, nuclear magnetic resonance, and fluorescence spectroscopic analysis. Recent advances in these methods, their limitations, and fields of application are discussed, with the aim of providing a guide for selecting the best method or combination of methods for lipid quantification.

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Effect of synthetic and natural media on lipid production from Fusarium oxysporum

Cited by 28 publications

References 62 publications

Heterotrophic cultivation of Auxenochlorella protothecoides using forest biomass as a feedstock for sustainable biodiesel production

Heterotrophic cultivation of Auxenochlorella protothecoides using forest biomass as a feedstock for sustainable biodiesel production

Physiological Traits of Dihomo-γ-Linolenic Acid Production of the Engineered Aspergillus oryzae by Comparing Mathematical Models

Lipids detection and quantification in oleaginous microorganisms: an overview of the current state of the art

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