Effect of surfactants on carryover liquid volume in immiscible phase magnetic bead separation

Troiano, Derek; Deraney, Rachel N.; Tripathi, Anubhav

doi:10.1016/j.colsurfa.2016.10.039

Cited by 14 publications

(11 citation statements)

References 26 publications

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“…A previous study also reported that PMP transfer was improved by reducing the surface energy of the interface . At the same time, this improvement can also prevent the magnetic beads from remaining at the interface position . Second, the square permanent magnet used in the previous study was replaced by a spherical permanent magnet.…”

Section: Resultsmentioning

confidence: 97%

Smartphone-Based Droplet Digital LAMP Device with Rapid Nucleic Acid Isolation for Highly Sensitive Point-of-Care Detection

Zhang

et al. 2019

Anal. Chem.

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While advances in microfluidics have enabled rapid and highly integrated detection of nucleic acid targets, the detection sensitivity is still unsatisfactory in the current POC (point-of-care) detection systems, especially for low abundance samples. In this study, a chip that integrates rapid nucleic acid extraction based on IFAST (immiscible phase filtration assisted by surface tension) and digital isothermal detection was developed to achieve highly sensitive POC detection within 60 min. Based on the interface theory, the factors influencing the interface stability of the IFAST process were studied, and the IFAST nucleic acid extraction conditions were optimized to increase the nucleic acid extraction recovery rate to 75%. Spiral mixing channel and flow-focusing droplet generation structure were designed to achieve the mixing and sample partitioning by applying negative pressure. A portable microdroplet fluorescence detection device was developed based on smartphone imaging. Validation tests were carried out for quantification of low-abundance cfDNA and detection of mutations.

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Section: Resultsmentioning

confidence: 97%

Smartphone-Based Droplet Digital LAMP Device with Rapid Nucleic Acid Isolation for Highly Sensitive Point-of-Care Detection

Zhang

et al. 2019

Anal. Chem.

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“…It is reported that the volume of carriers increases with the surface tension of the immiscible interface until it reaches a critical value. When the surface tension rises to a critical value, the volume of carriers remains constant, which will provide a guidance for the size of magnetic particles and the value of surface tension [69]. If the oil was not involved, the magnetic particles assisted solid phase extraction process would be simpler.…”

Section: The Water-oil Interface Based Nucleic Acid Purification Devicementioning

confidence: 99%

Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting

Chen

Liu

Shi

et al. 2020

TrAC Trends in Analytical Chemistry

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Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre -including this research content -immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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“…The microchannels are filled with oil to provide a phase separation of different fluid contents in the reservoirs. [12][13][14][15] A magnet external to the chip is used to manipulate the paramagnetic beads in the chip that transport targeted molecules. The magnetic particles are driven from one reservoir to next to provide a single-motion separation between bound (target) and unbound (undesired) molecules.…”

Section: Introductionmentioning

confidence: 99%

“…Bright field microscopy of reservoir 1 and the oil channel after DNA extraction and separation showed less than 1 ± 0.25% of beads remaining, and liquid carryover was determined to be very small in our previous work. 12…”

mentioning

confidence: 99%

A Microfluidics Workflow for Sample Preparation for Next-Generation DNA Sequencing

et al. 2019

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Next-generation sequencing technology requires amplified, short DNA fragments with known end sequences. Samples must undergo processing steps, including extraction and purification of genomic DNA (gDNA), fragmentation, end repair, adapter ligation, and amplification, to prepare a sequencing library. The process of sample preparation requires careful control of temperature and buffer conditions, as well as the stringent removal of contaminants. As a result, library preparation methods are often plagued by sample loss, long protocol times, numerous manual steps, and high cost. We attempt to understand and optimize each step of sample preparation on a microfluidic platform using magnetic bead motion through channels containing immiscible phases. Our platform integrates all steps associated with library preparation with no buffer exchanges and utilizes just 30-60 µL of reagents. Our chip shows a sixfold improvement in yield compared with an affinity spin column when capturing gDNA from samples of ~50 ± 4 MCF-7 cells. Finally, we show whole-genome shotgun sequencing results from 660 pg of human gDNA, in which >93 ± 1% of reads map to a reference genome at or above 99.9% confidence, matching a commercially available sample preparation kit optimized for low-cell-count samples.

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Effect of surfactants on carryover liquid volume in immiscible phase magnetic bead separation

Cited by 14 publications

References 26 publications

Smartphone-Based Droplet Digital LAMP Device with Rapid Nucleic Acid Isolation for Highly Sensitive Point-of-Care Detection

Smartphone-Based Droplet Digital LAMP Device with Rapid Nucleic Acid Isolation for Highly Sensitive Point-of-Care Detection

Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting

A Microfluidics Workflow for Sample Preparation for Next-Generation DNA Sequencing

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