Effect of surfactant on 5-aminolevulinic acid uptake and PpIX generation in human cholangiocarcinoma cell

Chung, Chung-Wook; Kim, Cy Hyun; Choi, Kyung Ha; Yoo, Jin-Ju; Kim, Do-Hyung; Chung, Kyudon; Jeong, Young‐Il; Kang, Dae Hwan

doi:10.1016/j.ejpb.2011.10.008

Cited by 18 publications

(22 citation statements)

References 30 publications

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“…CHO-K1 cells were chosen as a convenient model system for this study because the kinetics of PpIX production are well characterized. 39 In agreement with studies using other cell lines, 28,40 intracellular PpIX production reached a plateau when the 5-ALA concentration was > ~800 μM (Figure 2). To ensure that any additive enhancement effect was not masked by this PpIX plateau, a standard set of cell treatment conditions was employed (100 μM 5-ALA and 6 hour incubation time) for all experiments.…”

Section: Discussionsupporting

confidence: 87%

“…22 Non-covalent methods of 5-ALA delivery circumvent the need for intracellular esterases and proteases since they deliver unmodified 5-ALA that can directly enter the PpIX biosynthesis pathway. Different research groups have studied chitosan nanoparticles 23,24 and liposomes 25,26 loaded with 5-ALA to aid in intracellular delivery and, in some cases, transdermal delivery of 5-ALA. 23,26 The simplest chemical approach is to use an organic solvent such as DMSO (dimethylsulfoxide) 27 or a surfactant 28 as a formulation additive to enhance cell permeation. An obvious concern with this method is the potential for localized chemical toxicity or inflammation.…”

Section: Introductionmentioning

confidence: 99%

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Small molecule additive enhances cell uptake of 5-aminolevulinic acid and conversion to protoporphyrin IX

Harmatys

Musso

Clear

et al. 2016

Photochem Photobiol Sci

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Administration of exogenous 5-aminolevulinic acid (5-ALA) to cancerous tissue leads to intracellular production of photoactive protoporphyrin IX, a biosynthetic process that enables photodynamic therapy and fluorescence-guided surgery of cancer. Cell uptake of 5-ALA is limited by its polar structure and there is a need for non-toxic chemical additives that can enhance its cell permeation. Two zinc-bis(dipicolylamine) (ZnBDPA) compounds were evaluated for their ability to promote uptake of 5-ALA into Chinese Hamster Ovary (CHO-K1) cells and produce protoporphyrin IX. One of the ZnBDPA compounds was found to be quite effective, and a systematic comparison of cells incubated with 5-ALA (100 μM) for 6 hours showed that the presence of this ZnBDPA compound (10 μM) produced 3-fold more protoporphyrin IX than cells treated with 5-ALA alone. The results of mechanistic studies suggest that the ZnBDPA compound does not interact strongly with the 5-ALA. Rather, the additive is membrane active and transiently disrupts the cell membrane, permitting 5-ALA permeation. The membrane disruption is not severe enough to induce cell toxicity or allow passage of larger macromolecules like plasmid DNA.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Small molecule additive enhances cell uptake of 5-aminolevulinic acid and conversion to protoporphyrin IX

Harmatys

Musso

Clear

et al. 2016

Photochem Photobiol Sci

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“…When we studied the kinetics of PpIX fluorescence in PECA cells, it was found that PpIX production in PECA cells increased with the incubation time ( Figure 1). This result is consistent with other studies [42][43]. We also found that PpIX production in PECA cells didn't depend on ALA concentration.…”

Section: Discussionsupporting

confidence: 83%

Improvement of DC vaccine with ALA-PDT induced immunogenic apoptotic cells for skin squamous cell carcinoma

Wang

2015

Photodiagnosis and Photodynamic Therapy

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“…We previously reported that nonionic surfactants, such as Pluronic ® F68 or Tween ® 80 (Sigma-Aldrich), effectively enhance ALA uptake by tumor cells. 33 We suggest that PVA nanofibers are also effective vehicles for delivery of ALA.…”

Section: Discussionmentioning

confidence: 83%

5-aminolevulinic acid-incorporated poly(vinyl alcohol) nanofiber-coated metal stent for application in photodynamic therapy

Yoo¹,

Kim²,

Chung³

et al. 2012

IJN

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BackgroundThe study investigated the use of combined photodynamic therapy (PDT) and stent placement for the treatment of cholangiocarcinoma (CC). For this purpose, 5-aminolevulinic acid (ALA) was incorporated into poly(vinyl alcohol) (PVA) nanofiber, and coated onto metal stents. Their efficacy was assessed in PDT towards HuCC-T1 CC cells.MethodsFabrication of ALA-PVA nanofiber, and simultaneous coating onto metal stents, was performed through electrospinning. The dark-toxicity, generation of protoporphyrin IX (PpIX), and PDT effect of ALA and ALA-PVA nanofiber were studied in vitro, using HuCC-T1 CC cells.ResultsThe ALA-PVA nanofibers were coated onto metal stents less than 1000 nm in diameter. ALA-only displayed marginal cytotoxicity; ALA-PVA nanofiber showed less cytotoxicity. PpIX generation was not sigficantly different between ALA and ALA-PVA nanofiber treatments. PVA itself did not generate PpIX in tumor cells. ALA and ALA-PVA nanofiber displayed a similar PDT effect on tumor cells. Cell viability was decreased, dose-dependently, until ALA concentration reached 100 μg/mL. Necrosis and apoptosis of tumor cells occurred similarly for ALA and ALA- PVA nanofiber treatments.ConclusionThe ALA-PVA nanofiber-coated stent is a promising candidate for therapeutic use with cholangiocarcinoma.

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Effect of surfactant on 5-aminolevulinic acid uptake and PpIX generation in human cholangiocarcinoma cell

Cited by 18 publications

References 30 publications

Small molecule additive enhances cell uptake of 5-aminolevulinic acid and conversion to protoporphyrin IX

Small molecule additive enhances cell uptake of 5-aminolevulinic acid and conversion to protoporphyrin IX

Improvement of DC vaccine with ALA-PDT induced immunogenic apoptotic cells for skin squamous cell carcinoma

5-aminolevulinic acid-incorporated poly(vinyl alcohol) nanofiber-coated metal stent for application in photodynamic therapy

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