Effect of surface roughness on proliferation and alkaline phosphatase expression of rat calvarial cells cultured on polystyrene

Hatano, Kiyotoshi; Inoue, Hiromasa; Kojo, Tatsuro; Matsunaga, Tatsuaki; Tsujisawa, Toshiyuki; Uchiyama, Choji; Uchida, Yasunari

doi:10.1016/s8756-3282(99)00192-1

Cited by 201 publications

(152 citation statements)

References 23 publications

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“…That is, too many irregularities presented on the rough surface may make the cell hard to form enough number of stable contacts with the substrate. In the case of fetal rat calvarial cells, a similar trend in proliferation on a smooth and another four rough surfaces (R a : 0.37, 0.81, 1.70, 2.90 µm) was also reported [19], indicating the optimum average roughness of 0.81 µm. Deligianni, et al [20] found that the number of adherent human bone marrow cells per surface unit of Ti alloy increased with the increase of surface roughness in a narrow scope (R a : 0.32, 0.49 and 0.87 µm).…”

Section: Cell Proliferationsupporting

confidence: 53%

Effect of surface roughness on the initial response of MC3T3-E1 cells cultured on polished titanium alloy

Chen

Zheng

et al. 2015

BME

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Abstract. Surface roughness has been considered as an important influencing factor for cell response. The aim of this study was to find out whether MC3T3-E1 cells, a mouse osteoblast-like cell line, can sense the amplitudes of surface topography of titanium alloy (Ti6Al4V), and if surface-dependent cell morphology would be presented on the substrata with varied roughness. A series of polished samples (R a : 0.30~1.80 µm) were prepared to produce macroscopically parallel grooves using different grades of silicon carbide sandpaper (#100, #320, #600, #1000 and #2000). The experimental results indicated that the behavior and morphology of cells largely depended on the substrata where they were cultured. More efficient proliferation of MC3T3-E1 cells was shown on the surfaces with R a of 0.50~1.00 µm, with respect to either the rougher or the smoother specimens. Furthermore, MC3T3-E1 cells seeded on the Ti6Al4V surfaces within this narrow range responded to the increasing surface roughness with increased proliferation. Contact guidance of cells could be observed on the rougher specimens (R a : 0.80~1.00 µm), whereas more random orientations were exhibited for the adsorbed cells on the smoother surfaces (R a : 0.50~0.60 µm).

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Section: Cell Proliferationsupporting

confidence: 53%

Effect of surface roughness on the initial response of MC3T3-E1 cells cultured on polished titanium alloy

Chen

Zheng

et al. 2015

BME

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“…Significantly, these micro-scale geometric features influence osteoblast proliferation and differentiation in cell culture. For example, Hatano"s results [3]show that cellular proliferation levels increase in response to the increase in the degree of surface roughness up to 0.8 μm (Ra) and then decrease to the level observed for the smooth surface. The conclusions of Mustafa [4] indicate that the proliferation and differentiation of cells can be enhanced by increasing the particle size up to 300 m used in the blasting process.…”

Section: Introductionmentioning

confidence: 99%

“…Conventional surface texture characterisation parameters such as Ra, Rt and Rz [3] of a surface profile or Sa, Sds and Sdr [4,5] on an areal surface have been used to ascertain the functionality of titanium surfaces and have been proven to be of limited use. However, surfaces are complex entities which cannot be described completely by a single or even a few numerical descriptors [6].…”

Section: Introductionmentioning

confidence: 99%

Numerical characterisation of biomedical titanium surface texture using novel feature parameters

Wang

Jiang

Gurdak

et al. 2011

Wear

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In biomedicine, titanium materials with specific surface textures (for example, those produced using consecutive polishing, sandblasting and acid etching) are widely used to facilitate osseointegration of human/animal tissue with implant surface structures. The surface texture has a critical role on the material"s functionality in terms of cellular adhesion and proliferation. However, conventional surface topography characterisation parameters pertinent to cellular attachment such as Sds (density of summits of a surface) and Ssc (arithmetic mean summit curvature of a surface) are liable to be influenced by low amplitude high spatial frequency components or measurement noise. In this research, a novel feature characterisation, based on pattern recognition techniques is implemented on specially processed titanium surfaces. The mean dimensions and densities of the micro-scale features of the surfaces are extracted. The statistical analysis results demonstrate the efficiency and stability of the feature analysis compared to the use of conventional surface texture parameters. Additionally, potentially efficient parameters for characterisation of biomedical surfaces are indicated through the use of a one-way analysis of variance. As a case study, from the point of view of surface metrology, limited experimental data is presented; the intention of the authors is to give a guide to innovative use of the novel surface characterisation techniques. A large amount of biomedical experiments would be needed in the future to fully validate the correlation between the surface texture parameters and its biomedical functions but the present work provides a useful start point for a larger study.

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“…Some studies showed that the smooth surfaces favor the cellular proliferation [23,24]. However, De Santis et al [25] and Hatano et al [26] observed higher proliferation in cells plated onto roughness surfaces and Rosa and Beloti [27] did not verify the difference in this proliferation under roughness conditions. Although the cellular response in different surfaces is largely researched, the studies are limited by the use of the terms "smooth or rough", that is, surfaces with roughness values that little varies among them.…”

Section: Seven Daysmentioning

confidence: 97%

“…This change in the protocol was possible because of the greatest cautions taken during the surgical procedure, the development STEM CELLS BEHAVIOR ON TITANIUM SURFACES MODIFIED BY PLASMA Braz Dent Sci 2012 abr./jun. ; 15 (2) [19][20][21][22][23][24][25][26] of knowledge on the implant biomechanics, the modification in the implant design as well as the improvement of the techniques of modification of the implant surfaces [5]. The surface modification of this material results in a new Dentistry, where it is expected to decrease the non-functional time period of the implant, increase it applicability in alveolar bone with little quality, cause minimum discomfort to patient, and minimize the failure rates [1,[6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Stem cells behaviour on titanium surface modificated by plasma

Sá¹,

Silva²,

Rocha³

et al. 2012

BDS

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Over the last decade, a considerable development of new technologies to the modification of dental implants has been observed contributing to reduce the healing process and their use in areas with low bone density. Among the new techniques, plasma nitriding has showed excellent results. In this study, a superficial modification of commercial pure titanium (Degree II), by using two different plasma treatments (planar and hollow cathode nitriding) was accomplished aiming at an optimization of the surface for biomedical applications. An evaluation of the chemical composition in all samples was carried out, in addition to a study of their roughness and texture. Then, stem cells were deposited onto these surfaces and a comparison among their properties and their biological behavior was accomplished. The results showed that the nitriding techniques produced significant changes in the superficial texture of the Ti samples. The roughness test presented better results in samples nitrided by hollow cathode technique. Statistically significant differences were found in the cell proliferation of samples nitrided by hollow cathode nitriding when compared to samples without treatment. The used techniques were, therefore, effective and directly influenced the characteristics of the titanium surface and consequently, the stem cell behavior.

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Effect of surface roughness on proliferation and alkaline phosphatase expression of rat calvarial cells cultured on polystyrene

Cited by 201 publications

References 23 publications

Effect of surface roughness on the initial response of MC3T3-E1 cells cultured on polished titanium alloy

Effect of surface roughness on the initial response of MC3T3-E1 cells cultured on polished titanium alloy

Numerical characterisation of biomedical titanium surface texture using novel feature parameters

Stem cells behaviour on titanium surface modificated by plasma

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