Effect of surface roughness on performance of magnetoelastic biosensors for the detection of Escherichia coli

A magnetoelastic (ME) biosensor for wireless detection of analytes in liquid is described. The ME biosensor was tested against human IgG in the range 0–20 μg∙mL−1. The sensing elements, anti-human IgG produced in goat, were immobilized on the surface of the sensor by using a recently introduced photochemical immobilization technique (PIT), whereas a new amplification protocol exploiting gold coated magnetic nanoparticles (core-shell nanoparticles) is demonstrated to significantly enhance the sensitivity. The gold nanoflowers grown on the magnetic core allowed us to tether anti-human IgG to the nanoparticles to exploit the sandwich detection scheme. The experimental results show that the 6 mm × 1 mm × 30 μm ME biosensor with an amplification protocol that uses magnetic nanoparticles has a limit of detection (LOD) lower than 1 nM, works well in water, and has a rapid response time of few minutes. Therefore, the ME biosensor is very promising for real-time wireless detection of pathogens in liquids and for real life diagnostic purpose.

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“…Compared with other works found in literature [ 38 , 39 ], polishing and annealing of the ME ribbons were not needed in our procedure.…”

Section: Methodsmentioning

confidence: 93%

Core-Shell Magnetic Nanoparticles for Highly Sensitive Magnetoelastic Immunosensor

et al. 2020

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“…This coating can be an enzyme or molecule that specifically binds with an analyte, a coating that absorbs/desorbs water as pH changes, or molecules that are specifically consumed during reactions/metabolism. Functionalization of magnetoelastic sensors has increased their popularity as a platform for label-free detection [ 5 ] and has facilitated the detection and measurement of chemical analytes, such as pH [ 2 ], ammonia [ 2 ], carbon dioxide [ 2 ], calcium oxalate precipitate [ 6 ], and octachlorostyrene [ 7 ], and biologicals and biotoxins such as E. coli , Staphylococcus enterotoxin B, and other endotoxins [ 8 , 9 , 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Magnetoelastic Sensor Optimization for Improving Mass Monitoring

Skinner

Zhang

Guldberg

et al. 2022

Sensors

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Magnetoelastic sensors, typically made of magnetostrictive and magnetically-soft materials, can be fabricated from commercially available materials into a variety of shapes and sizes for their intended applications. Since these sensors are wirelessly interrogated via magnetic fields, they are good candidates for use in both research and industry, where detection of environmental parameters in closed and controlled systems is necessary. Common applications for these sensors include the investigation of physical, chemical, and biological parameters based on changes in mass loading at the sensor surface which affect the sensor’s behavior at resonance. To improve the performance of these sensors, optimization of sensor geometry, size, and detection conditions are critical to increasing their mass sensitivity and detectible range. This work focuses on investigating how the geometry of the sensor influences its resonance spectrum, including the sensor’s shape, size, and aspect ratio. In addition to these factors, heterogeneity in resonance magnitude was mapped for the sensor surface and the effect of the magnetic bias field strength on the resonance spectrum was investigated. Analysis of the results indicates that the shape of the sensor has a strong influence on the emergent resonant modes. Reducing the size of the sensor decreased the sensor’s magnitude of resonance. The aspect ratio of the sensor, along with the bias field strength, was also observed to affect the magnitude of the signal; over or under biasing and aspect ratio extremes were observed to decrease the magnitude of resonance, indicating that these parameters can be optimized for a given shape and size of magnetoelastic sensor.

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“…Several authors have used magneto-elastic alloys in the study of specific properties of coatings, such as mechanical behavior [13], water absorption [14] and magnetic coupling [15]. In addition, several researches have been carried out with the use of this metal alloy as a biosensor platform for the detection of microorganisms [16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Monitoring the electrochemical behavior of hybrid coatings on magneto-elastic sensors using resonant frequency variations

Beltrami

Missell

et al. 2021

Materials Science and Engineering: B

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In this work we present results for the protective performance of coatings used on magneto-elastic ribbons. It is found that monitoring the resonance frequency variation (Δf) of a magneto-elastic sensor can give useful electrochemical information. This method has the advantage of allowing one to monitor the electrochemical behavior of coatings without the need for direct access to the sample. A sensor developed from the FeNiMoB alloy (Metglas 2826MB3) was covered with hybrid coatings based on silicon alkoxide precursors. The results obtained from the magneto-elastic sensor test were correlated with the results obtained from electrochemical impedance spectroscopy (EIS). In general, it was observed that the Δf monitoring technique provided results consistent with those obtained by EIS, with the advantage that it does not need to have direct contact with the sample. Besides that monitoring the resonance frequency also allows one to evaluate the electrochemical behavior of the coating in the medium of interest.

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Effect of surface roughness on performance of magnetoelastic biosensors for the detection of Escherichia coli

Cited by 20 publications

References 31 publications

Core-Shell Magnetic Nanoparticles for Highly Sensitive Magnetoelastic Immunosensor

Core-Shell Magnetic Nanoparticles for Highly Sensitive Magnetoelastic Immunosensor

Magnetoelastic Sensor Optimization for Improving Mass Monitoring

Monitoring the electrochemical behavior of hybrid coatings on magneto-elastic sensors using resonant frequency variations

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