Effect of Surface Morphology and Charge on the Amount and Conformation of Fibrinogen Adsorbed onto Alginate/Chitosan Microcapsules

Xie, Hong; Zheng, Juanjuan; Li, Xiao X.; Liu, Xiu D.; Zhu, Jing; Wang, Feng; Xie, Wei; Xiao, Jianru

doi:10.1021/la903874g

Cited by 37 publications

(32 citation statements)

References 67 publications

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“…Yet, roughness is only a single measure of the topography of a surface, and despite many studies there appears to be no single unifying description of how roughness affects binding (1,2,(52)(53)(54). Our experiments are performed on surfaces with rms roughness between 1.1 nm and 0.18 nm (PD and SCD), considerably smaller than the rms roughness in most other studies (53,55).…”

Section: Discussionmentioning

confidence: 99%

Surface functionalization of thin-film diamond for highly stable and selective biological interfaces

Stavis

Clare

Butler

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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Carbon is an extremely versatile family of materials with a wide range of mechanical, optical, and mechanical properties, but many similarities in surface chemistry. As one of the most chemically stable materials known, carbon provides an outstanding platform for the development of highly tunable molecular and biomolecular interfaces. Photochemical grafting of alkenes has emerged as an attractive method for functionalizing surfaces of diamond, but many aspects of the surface chemistry and impact on biological recognition processes remain unexplored. Here we report investigations of the interaction of functionalized diamond surfaces with proteins and biological cells using X-ray photoelectron spectroscopy (XPS), atomic force microscopy, and fluorescence methods. XPS data show that functionalization of diamond with short ethylene glycol oligomers reduces the nonspecific binding of fibrinogen below the detection limit of XPS, estimated as >97% reduction over H-terminated diamond. Measurements of different forms of diamond with different roughness are used to explore the influence of roughness on nonspecific binding onto H-terminated and ethylene glycol (EG)-terminated surfaces. Finally, we use XPS to characterize the chemical stability of Escherichia coli K12 antibodies on the surfaces of diamond and amine-functionalized glass. Our results show that antibody-modified diamond surfaces exhibit increased stability in XPS and that this is accompanied by retention of biological activity in cell-capture measurements. Our results demonstrate that surface chemistry on diamond and other carbonbased materials provides an excellent platform for biomolecular interfaces with high stability and high selectivity.biointerfaces | surface chemistry | cells

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Section: Discussionmentioning

confidence: 99%

Surface functionalization of thin-film diamond for highly stable and selective biological interfaces

Stavis

Clare

Butler

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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“…During the membrane formation process, strong attractive forces between the nearest opposite charge groups in the polymer chains caused the contraction and aggregation of the gel skeleton as shown in Figure . The space rearrangement of gel skeleton induced the formation of big aggregates and pores on AC 1 membrane surface, which was consistent with the results observed by previous papers . However, the rougher and thicker AC 1 membrane with porous structure hardly obstructed the inward diffusion of C 2 molecules [Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The surface roughness of flat membrane was measured in the wet state by a noncontact, three-dimensional optical interferometer (New-View 5020, ZYGO). 28 Before starting the measurement, free water on the surface of membranes was removed with absorbent article from one side of the gel membrane. Average values were obtained from multiple roughness values (at least three) on different areas of each sample.…”

Section: Characterization Of the Surface Morphology Of Pec Hydrogel Mmentioning

confidence: 99%

The cause and influence of sequentially assembling higher and lower deacetylated chitosans on the membrane formation of microcapsule

Zheng

Xie

et al. 2015

J Biomedical Materials Res

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Alginate-chitosan (AC) microcapsules with desired strength and biocompatibility are preferred in cell-based therapy. Sequential assembly of higher and lower deacetylated chitosans (C1 and C2 ) on alginate has produced AC1 C2 microcapsule with improved membrane strength and biocompatibility. In this article, the assembly and complexation processes of two cationic chitosans on anionic alginate were concerned, and the cause and influence of sequentially assembling chitosans on AC1 C2 microcapsules membrane formation were evaluated. It was found that C1 complexation was the key factor for deciding the membrane thickness of AC1 C2 microcapsule. Specifically, the binding amount of C2 positively related to the binding amount of C1 , which suggested the first layer by C1 complexation on alginate had no obvious resistance on the sequential cationic C2 complexation. Further analyses demonstrated that outward migration of alginate molecules and inward diffusion of both chitosans under electrostatic interaction contributed to the sequential coating of C2 on first C1 layer. Moreover, C2 complexation through the surface to inner layer of membrane helped smoothen the first layer by C1 complexation that displayed a synergy role on the formation of AC1 C2 microcapsule membrane. Therefore, the two chitosans played different roles and synergistically contributed to membrane properties that can be easily regulated with membrane complexation time.

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“…As the electrolyte is introduced into the cell, the surface charges on the microcapsules are disrupted, causing a potential difference, i.e. the streaming potential (Xie et al, 2010). Electrophoresis uses an electric field to force charged particles to move in a surrounding liquid.…”

Section: Surface Charge and Zeta Potentialmentioning

confidence: 99%

Determination of microcapsule physicochemical, structural, and mechanical properties

et al. 2016

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a b s t r a c tResearch into the fundamental properties of microcapsules and use of the results to develop a wide variety of products in industries such as printing, fast-moving consumer goods, construction, pharmaceuticals, and agrochemicals is a dynamic and ever-progressing field of study. For microcapsules to be effective in providing protection from harsh environments or delivering large payloads, it is essential to have a good understanding of their properties to enable quality control during formulation, storage, and applications. This review aims to outline the commonly used techniques for determining the physicochemical, structural, and mechanical properties of microcapsules, and highlights the interlinked nature of these three areas with respect to the end-use industrial application. This review provides information on techniques that are well supported in the literature, and also examines microcapsule analytical techniques that will become more prevalent as a result of new technological developments or extensions from other areas of study.

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Effect of Surface Morphology and Charge on the Amount and Conformation of Fibrinogen Adsorbed onto Alginate/Chitosan Microcapsules

Cited by 37 publications

References 67 publications

Surface functionalization of thin-film diamond for highly stable and selective biological interfaces

Surface functionalization of thin-film diamond for highly stable and selective biological interfaces

The cause and influence of sequentially assembling higher and lower deacetylated chitosans on the membrane formation of microcapsule

Determination of microcapsule physicochemical, structural, and mechanical properties

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