Effect of Surface Charge on the Uptake of Magnetic Nanoparticles in Mouse Fibroblast Cells

Matahum, Jefunnie; Su, Chao-Ming; Wang, Weijie; Lou, Shyh-Liang; Ger, Tzong-Rong

doi:10.1109/lmag.2016.2629458

Cited by 12 publications

(9 citation statements)

References 27 publications

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“…The particle size distribution shows that before purification, the sample exhibited significant clustering with particles that even reached the micron size range. Similar distributions have been observed previously for materials with different metal components due to different surface charge values [ 22 , 38 ]. The FTIR data in Figure 3 A confirmed the purification process’s effectiveness as peaks associated with organic compounds significantly decreased or even disappeared.…”

Section: Resultssupporting

confidence: 87%

Patchy Core/Shell, Magnetite/Silver Nanoparticles via Green and Facile Synthesis: Routes to Assure Biocompatibility

et al. 2020

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Nanomedicine is entering a high maturity stage and is ready to reach full translation into the clinical practice. This is because of the ample spectrum of applications enabled by a large arsenal of nanostructured materials. In particular, bimetallic patchy core/shell nanoparticles offer tunable surfaces that allow multifunctional responses. Despite their attractiveness, major challenges regarding the environmental impact and biocompatibility of the obtained materials are yet to be solved. Here, we developed a green synthesis scheme to prepare highly biocompatible patchy core/shell magnetite/silver nanoparticles for biological and biomedical applications. The magnetite core was synthesized by the co-precipitation of ferric chloride and ferrous chloride in the presence of NaOH. This was followed by the patchy silver shell’s growth by a green synthesis approach based on natural honey as a reducing agent. A purification process allowed selecting the target patchy nanoparticles and removing excess toxic reagents from the synthesis very efficiently. The obtained patchy magnetite/silver nanoparticles were characterized by UV-Vis spectrophotometry, dynamic light scattering (DLS), thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope equipped with energy-dispersive spectroscopy (SEM + EDS), and transmission electron microscopy (TEM). The morphology, patchiness level, and size of the nanoparticles were determined via SEM and TEM. In addition, the spectrophotometric characterization confirmed the presence of the patchy silver coating on the surface of the magnetite core. The nanoparticles show high biocompatibility, as evidenced by low cytotoxicity, hemolytic effect, and platelet aggregation tendency. Our study also provides details for the conjugation of multiples chemistries on the surface of the patchy bimetallic nanoparticles, which might be useful for emerging applications in nanomedicine, where high biocompatibility is of the utmost importance.

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Section: Resultssupporting

confidence: 87%

Patchy Core/Shell, Magnetite/Silver Nanoparticles via Green and Facile Synthesis: Routes to Assure Biocompatibility

et al. 2020

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“…Magnetite was synthesized by co-precipitation, which has been reported as one of the simplest synthesis methods [39,40]. Even though the particle size distribution obtained is ampler than with other methods [41], co-precipitation is an attractive route from the scaling-up viewpoint as no expensive reagents or extreme process conditions are required. Additionally, the silver shell was synthesized by a "green synthesis" scheme with commercially available honey as a reducing agent [37].…”

Section: Resultsmentioning

confidence: 99%

“…Figure 2 B shows the obtained Zeta-potentials in the pH range from 6 to 8. In all cases, the Zeta-potential remains negative, which may be useful to improve nanomaterial cellular uptake [ 25 , 41 ]. Also, it shows how both magnetite and magnetite/silver exhibit similar behavior.…”

Section: Resultsmentioning

confidence: 99%

PH-Responsive, Cell-Penetrating, Core/Shell Magnetite/Silver Nanoparticles for the Delivery of Plasmids: Preparation, Characterization, and Preliminary In Vitro Evaluation

et al. 2020

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Over the past decade, gene therapies have attracted much attention for the development of treatments for various conditions, including cancer, neurodegenerative diseases, protein deficiencies, and autoimmune disorders. Despite the benefits of this approach, several challenges are yet to be solved to reach clinical implementation. Some of these challenges include low transfection rates, limited stability under physiological conditions, and low specificity towards the target cells. An avenue to overcome such issues is to deliver the therapies with the aid of potent cell-penetrating vectors. Non-viral vectors, such as nanostructured materials, have been successfully tested in drug and gene delivery. Here, we propose the development and in vitro evaluation of a nanostructured cell-penetrating vehicle based on core/shell, magnetite/silver nanoparticles. A subsequent conjugation of a pH-responsive polymer was used to assure that the vehicle can carry and release circular DNA. Additionally, the translocating peptide Buforin II was conjugated with the aid of a polyether amine polymer to facilitate translocation and endosome escape. The obtained nanobioconjugates (magnetite/silver-pDMAEMA-PEA-BUFII) were characterized by UV-Vis spectrophotometry, dynamic light scattering (DLS), thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope equipped with energy dispersive spectroscopy (SEM+EDS), and transmission electron microscopy (TEM). They were also encapsulated in lecithin liposomes to form magnetoliposomes. The cell viability of Vero cells in the presence of the nanobioconjugates was above 95% and declined to 80% for the magnetoliposomes. The hemolytic tendency of nanobioconjugates and magnetoliposomes was below 10%, while the platelet aggregation approached that of the negative control (i.e., 35%). Cytoplasm coverage values of about 50% for both Vero and neuroblastoma cells confirmed significant cell penetration. Pearson’s correlation coefficients for both cell lines allowed us to estimate 20–40% colocalization of the nanobioconjugates with lysotracker green, which implied high levels of endosomal escape. The developed vehicles were also capable of loading around 16% of the added DNA and releasing such cargo with 8% efficiency. The developed nanoplatform holds a significant promise to enable highly efficient gene therapies as it overcomes some of the major issues associated with their eventual translation to the pre-clinical and clinical scale.

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“…The properties of nanoparticles depend mainly on the nature of their synthesis. Co-precipitation and thermal decomposition are currently the most common chemical methods to obtain MNPs for various applications notably in nanobiomedicine, due to their potential to achieve large quantity and desired size, morphology, structure and magnetic control [38]. Although thermal decomposition can produce MNPs of greater quality than co-precipitation, the requirement of temperature in a range between 200°C and 300°C for reaction and the as-prepared MNPs only dispersible in organic solvents limit its applications in the field of biology.…”

Section: Discussionmentioning

confidence: 99%

Synthesis and characterization of magnetic nanoparticles coated with polystyrene sulfonic acid for biomedical applications

Chen

Sung

et al. 2020

Science and Technology of Advanced Materials

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The development of novel magnetic nanoparticles (MNPs) with satisfactory biocompatibility for biomedical applications has been the subject of extensive exploration over the past two decades. In this work, we synthesized superparamagnetic iron oxide MNPs coated with polystyrene sulfonic acid (PSS-MNPs) and with a conventional co-precipitation method. The core size and hydrodynamic diameter of the PSS-MNPs were determined as 8-18 nm and 50-200 nm with a transmission electron microscopy and dynamic light scattering, respectively. The saturation magnetization of the particles was measured as 60 emu g −1 with a superconducting quantum-interference-device magnetometer. The PSS content in the PSS-MNPs was 17% of the entire PSS-MNPs according to thermogravimetric analysis. Fouriertransform infrared spectra were recorded to detect the presence of SO 3 − groups, which confirmed a successful PSS coating. The structural properties of the PSS-MNPs, including the crystalline lattice, composition and phases, were characterized with an X-ray powder diffractometer and 3D nanometer-scale Raman microspectrometer. MTT assay and Prussian-blue staining showed that, although PSS-MNPs caused no cytotoxicity in both NIH-3T3 mouse fibroblasts and SK-HEP1 human liver-cancer cells up to 1000 μg mL −1 , SK-HEP1 cells exhibited significantly greater uptake of PSS-MNPs than NIH-3T3 cells. The low cytotoxicity and high biocompatibility of PSS-MNPs in human cancer cells demonstrated in the present work might have prospective applications for drug delivery.

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Effect of Surface Charge on the Uptake of Magnetic Nanoparticles in Mouse Fibroblast Cells

Cited by 12 publications

References 27 publications

Patchy Core/Shell, Magnetite/Silver Nanoparticles via Green and Facile Synthesis: Routes to Assure Biocompatibility

Patchy Core/Shell, Magnetite/Silver Nanoparticles via Green and Facile Synthesis: Routes to Assure Biocompatibility

PH-Responsive, Cell-Penetrating, Core/Shell Magnetite/Silver Nanoparticles for the Delivery of Plasmids: Preparation, Characterization, and Preliminary In Vitro Evaluation

Synthesis and characterization of magnetic nanoparticles coated with polystyrene sulfonic acid for biomedical applications

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