Effect of suramin on trypomastigote forms of Trypanosoma cruzi: Changes on cell motility and on the ultrastructure of the flagellum-cell body attachment region

Bisaggio, Danielle F. R.; Campanati, Loraine; Pinto, Rodrigo Coelho Ventura; Souto-Padrón, Thaïs

doi:10.1016/j.actatropica.2006.04.003

Cited by 23 publications

(30 citation statements)

References 36 publications

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“…Treatment of T. cruzi with suramin, a symmetrical polysulfonated derivative of urea used in the prophylactic treatment of human trypanosomiasis in Africa, caused increased activity of a Mg 2+ ecto-ATPase and the FRA antigen and redistribution of negative surface charges. Alterations in extracellular components in the region of adhesion between the cell body and the flagellum and an increase in the adhesion of epimastigotes to resident macrophages were also observed [92,98,99] (Fig. 2L). …”

Section: The Cell Surfacementioning

confidence: 74%

Contributions of Ultrastructural Studies to the Cell Biology of Trypanosmatids: Targets for Anti-Parasitic Drugs

Almeida¹,

Souto-Padrón

2010

TOPARAJ

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Protozoan parasites cause disease in humans worldwide, and many fall into the genera Trypanosoma and Leishmania; these parasites are responsible for African trypanosomiasis, Chagas disease and the different forms of Leishmaniasis. Strategies for the development of new drugs against these protozoans have been based on their cell biology and biochemistry complemented by the use of electron microscopy. Trypanosoma and Leishmania have special organelles that are involved in metabolic pathways, which are very distinct from those in mammalian cells; these organelles are potential drug targets. Scanning and transmission electron microscopy can identify not only the target organelles but also alterations to the cell surface and ultrastructural changes that characterize distinct forms of programmed cell death.

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Section: The Cell Surfacementioning

confidence: 74%

Contributions of Ultrastructural Studies to the Cell Biology of Trypanosmatids: Targets for Anti-Parasitic Drugs

Almeida¹,

Souto-Padrón

2010

TOPARAJ

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“…Moreover, prolonged incubation of T. cruzi-infected LLC-MK2 cells in the presence of suramin (500 μM) causes morphological changes on trypomastigote forms characterized by an accentuated decrease on parasite motility [49]. In trypomastigotes, suramin causes a decrease in ~5% in cell length and an increase in ~43% in cell width [49]. Also, it was observed that 95% of trypomastigotes exposed to suramin present a partial or even total detachment of the flagellum from the cell body [49].…”

Section: Functional Studiesmentioning

confidence: 99%

“…In trypomastigotes, suramin causes a decrease in ~5% in cell length and an increase in ~43% in cell width [49]. Also, it was observed that 95% of trypomastigotes exposed to suramin present a partial or even total detachment of the flagellum from the cell body [49].…”

Section: Functional Studiesmentioning

confidence: 99%

“…Also, suramin (300 μM for 12 h) did not affect the total Cx43 level [48]. Moreover, prolonged incubation of T. cruzi-infected LLC-MK2 cells in the presence of suramin (500 μM) causes morphological changes on trypomastigote forms characterized by an accentuated decrease on parasite motility [49]. In trypomastigotes, suramin causes a decrease in ~5% in cell length and an increase in ~43% in cell width [49].…”

Section: Functional Studiesmentioning

confidence: 99%

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Involvement of Gap Junction Proteins in Infectious Diseases Caused by Parasites

Vega¹,

Barría²,

Güiza³

et al. 2017

Natural Remedies in the Fight Against Parasites

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Parasitic diseases affect low-income nations with health consequences that affect the economy of these countries. Research aimed at understanding their biology and identification of potential targets for drug development is of the highest priority. Inhibitors of channels formed by proteins of the gap junction family such as suramin and probenecid are currently used for treatment of parasitic diseases caused by pathogenic protozoan. Gap junction proteins are present in both vertebrates and invertebrates permitting direct and indirect cellular communication. These cellular specializations are formed by two protein families corresponding to connexins (vertebrates) and innexins (invertebrates). In addition, a third protein family composed by proteins denominated pannexins is present in vertebrates and shows primary sequence homology to innexins. Channels formed by these proteins are essential in many biological processes. Recent evidences suggest that gap junction proteins play a critical role in bacterial and viral infections. Nonetheless, little is known about the role of these channels in parasitic infections. In this chapter, we summarized the current knowledge about the role of gap junction family proteins and channels in parasitic infections.

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“…It has been reported that in T. cruzi, suramin can inhibit various enzymes, the endocytosis of some molecules, and interfere with the process of cell division 25 . Bisaggio et al 26 showed modulatory effect of suramin on enzymatic activity of the ecto-ATPase Mg 2+ dependent on the surface of T. cruzi. This inhibition was dose-dependent, and an important biological consequence reported is the accentuated reduction of the ATP hydrolysis rate and a significant reduction in the electrondense reaction product observed on the cell surface of epimastigote and trypomastigote forms of the parasite.…”

Section: All Authors Contributed To Conception and Design Manuscriptmentioning

confidence: 99%

Implication of purinergic signaling pathways in clinical management of Chagas' disease

Santos¹,

Novaes²,

Cardoso³

et al. 2013

OA Biotechnology

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Effect of suramin on trypomastigote forms of Trypanosoma cruzi: Changes on cell motility and on the ultrastructure of the flagellum-cell body attachment region

Cited by 23 publications

References 36 publications

Contributions of Ultrastructural Studies to the Cell Biology of Trypanosmatids: Targets for Anti-Parasitic Drugs

Contributions of Ultrastructural Studies to the Cell Biology of Trypanosmatids: Targets for Anti-Parasitic Drugs

Involvement of Gap Junction Proteins in Infectious Diseases Caused by Parasites

Implication of purinergic signaling pathways in clinical management of Chagas' disease

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