Effect of supplementation of various selenium forms and doses on selected parameters of ruminal fluid and blood in sheep

Panev, Aleksandar; Hauptmanová, K.; Pavlata, L.; Pechová, Alena; Filípek, J.; Dvořák, R.

doi:10.17221/6524-cjas

Cited by 29 publications

(24 citation statements)

References 46 publications

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“…Previous findings (Mihaliková et al 2005;Panev et al 2013) have shown that rumen microbial population is capable of incorporating Se into their cells. Several studies have dealt with the effects of various Se forms on the rumen enzyme activity.…”

Section: Rumen Blood Enzymes Ruminantmentioning

confidence: 92%

“…In addition, the chemical form of Se in the supplement did not affect ruminal fermentation indicators in dependence on different doses of Se contained in feeding rations. However, a negative effect of a sudden start of feeding high contents of organically bound Se on the infusoria count was reported, which was accompanied by the increase of GMT, LDH and AST enzymes activity in ruminal fluid (Panev et al 2013).…”

Section: Rumen Blood Enzymes Ruminantmentioning

confidence: 99%

“…selenium concentration in the rumen fluid was significantly higher in the Se-yeast group. Selenium added to the feeding ration is not fully utilized by the animal's organism, as its significant part is absorbed and metabolized by ruminal bacteria, thus the highest selenium concentrations are found in ruminal biomass, whereas the lowest are in the ruminal fluid (Serra et al 1994;Panev et al 2013).…”

Section: Rumenmentioning

confidence: 99%

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Effect of dietary supplementation with selenium-enriched yeast or sodium selenite on ruminal enzyme activities and blood chemistry in sheep

et al. 2016

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The experiment was conducted to evaluate the effect of feeding a diet supplemented with different forms of selenium on the rumen fluid, blood and serum enzyme activity and osmotic fragility of red blood cells in sheep. The experiment was carried out on 18 sheep of the Valashka breed at the age of 18 months, divided into 3 groups. The first group was given basal diet (BD) with a Se content of 0.17 mg/kg of dry matter (DM). The second group received BD supplemented with 0.4 mg Se/kg of (DM) in the form of sodium selenite. The third group received BD supplemented with 0.4 mg Se/kg of (DM) in the form of Se-yeast extract. Duration of the trial was 12 weeks. Selenium concentration in blood and total rumen fluid were elevated in both supplemented groups with the highest values in Se-yeast-treated sheep. Blood glutathione peroxidase (GPx) activity was significantly increased, regardless of the source of selenium. Osmotic resistance of red blood cells was not affected by selenium supplementation. The results indicate that feeding a diet supplemented with selenium from Se-yeast or selenite improved selenium status in blood and total rumen fluid. Selenium from sodium selenite was as effective as selenium from Se-yeast in the availability of selenium for the blood GPx activity. The effect of selenium supplementation on the ruminal enzyme activity depends on the selenium form; GGT and GDH were significantly higher in the Se-yeast supplement group, AST and ALP were significantly higher in the selenite supplement group. Rumen, blood enzymes, ruminantSelenium is an essential trace element which plays an important role in the antioxidative, reproductive, endocrine and immune systems of species.In most European Union countries the natural Se content in feed is only 0.03-0.12 mg/kg of dry matter (Pappas et al. 2008). The intake of such feeds can result in serious Se deficiencies and health problems especially in young growing and highly producing animals.For this reason commercial animal feeds are routinely supplemented with various Se sources up to the maximum European Union authorized total Se content of 0.5 mg/kg of feed.Currently, sodium selenite (Na 2 SeO 3 ) and sodium selenate (Na 2 SeO 4 ) as inorganic and selenium-enriched yeast (Se-yeast) as organic Se are principal selenium supplements of animal feed.It is established that selenocompounds from dietary inorganic and organic Se sources follow different metabolic pathways (Qin et al. 2007;Wang et al. 2009). Intestinal absorption of Se is much lower in ruminants than in nonruminants. For selenite, the absorption is 79% and 80% in poultry and swine, respectively, whereas only 29% in sheep. For selenomethionine and selenate the absorption is higher than 90% in monogastrics and poultry. Low absorption of selenium in ruminants is believed to result from reduction of dietary selenium (selenite and selenate) to unsoluble forms such as elemental selenium or selenides in the rumen environment (Mehdi et al. 2013).

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Section: Rumen Blood Enzymes Ruminantmentioning

confidence: 92%

Section: Rumen Blood Enzymes Ruminantmentioning

confidence: 99%

Section: Rumenmentioning

confidence: 99%

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Effect of dietary supplementation with selenium-enriched yeast or sodium selenite on ruminal enzyme activities and blood chemistry in sheep

et al. 2016

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“…In the study on pregnant goats Pavlata et al (2012) found that all the above forms of selenium (sodium selenite, lactate-protein selenium complex, selenium proteinate, and selenium yeasts) were similarly utilized and transferred into the fetus in the goats. The results of the study by Panev et al (2013) demonstrate that the actual intake of both organic and inorganic selenium is reflected in selenium concentration in ruminal fluid and ruminal biomass and, similarly, in selenium content and GSH-Px activity in blood. The form of supplemented selenium did not have a significant effect on ruminal fermentation parameters.…”

Section: Discussionmentioning

confidence: 87%

“…It is reported that the advantage of organically bound Cu is its greater solubility and ability to better withstand negative interactions with the antagonists (Brown and Zeringue, 1994). Panev et al (2013) however suggested possible negative interaction between the intake of organically bound selenium and the concentration of Cu in blood of sheep. Zeng and Botnen (2004) described that selenite and selenocysteine can cause cell cycle arrest via distinct mechanisms, and suggest that Cu may interact with selenite extracellularly, which represents the basis of antagonism between copper and selenite.…”

Section: Discussionmentioning

confidence: 99%

Copper metabolism in goat-kid relationship at supplementation of inorganic and organic forms of copper

Páleníková¹,

Hauptmanová²,

Pitropovská³

et al. 2014

CZECH J ANIM SCI

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AbstrACt:The aim of the experiment was to compare the effect of inorganic and organic forms of copper (Cu) supplementation on Cu status of goats and their kids, colostrum and milk composition and quality, and on the Cu concentrations in amniotic fluids and fetal membranes. The experiment involved 22 clinically healthy pregnant goats with similar mean Cu concentration in blood serum. Goats were divided into 3 groups: E1, E2, and C. Basal feed ration differed only in Cu form and concentration in a grain mixture. The goats of experimental groups E1 and E2 received the supplement of Cu sulfate and Cu chelate, respectively. Control group C was without Cu supplementation. Blood samples from goats and kids were collected on the day of parturition (day 0; in kids before colostrum intake) and on days 2, 7, and 21 postpartum. On the same days the kids were weighed. Colostrum or milk samples were collected on days 0, 5, and 30. During delivery, also samples of amniotic fluids and fetal membranes were collected. Both forms of Cu supplementation resulted in higher average concentration of Cu (compared to control group) in blood serum of goats (19.5 ± 1.7 and 18.5 ± 2.5 vs. 15.2 ± 4.4 µmol/l, respectively) and blood serum of kids (6.7 ± 0.8 and 6.0 ± 0.5 vs. 6.0 ± 1.0 µmol/l, respectively). Significant differences in Cu serum concentration on the day of parturition in goats were observed in group E1 compared to control (P < 0.05) and also in kids of group E1 compared to group E2 and control (P < 0.05). The Cu concentration in the blood of kids on the day of parturition was significantly (P < 0.01) lower compared to that in maternal blood. Percentages of Cu concentration in the blood of kids in groups E1, E2, and C were 34, 33, and 39% of that in maternal blood. The results of Cu concentration in blood serum of goats on days 2, 7, and 21 were without significant differences between groups. Significantly higher Cu serum concentrations (P < 0.05) were observed in kids on day 2 in group E1 compared to control group and also on day 21 in group E1 compared to group E2. Colostrum Cu concentration was significantly (P < 0.05) higher in group E1 (10.6 ± 3.3 µmol/l) compared to group E2 (7.1 ± 1.5 µmol/l). There were no significant differences observed in Cu concentration in amniotic fluids and fetal membranes. The kids on both forms of Cu supplementation (on day 0 in group E1 and on days 2, 7, and 21 in group E2) had significantly (P < 0.05) higher average weight than the kids from control group. Our results are suggesting that the inorganic form of Cu (copper sulfate) is more efficient than organic (copper chelate) in influencing the Cu metabolism in goat-kid relationship and that Cu supplementing has a positive effect on the weight of kids.

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Effects of Different Dietary Selenium Sources on Antioxidant Status and Blood Phagocytic Activity in Sheep

Čobanová

Faix

Plachá

et al. 2016

Biol Trace Elem Res

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The objective of this experiment was to investigate the effects of feed supplementation with equivalent doses of selenium from sodium selenite (SS) or selenized yeast (SY) on Se deposition, selenoenzyme activity and lipid peroxidation in tissues as well as in bacterial and protozoal fractions of rumen contents in sheep. The phagocytic activity of monocytes and neutrophils in whole blood was also assessed after 3 months of dietary treatment. While animals in the control group were fed with unsupplemented basal diet (BD) containing only background Se (0.16 mg/kg DM), the diet of the other two groups (n = 6) consisted of identical BD enriched with 0.4 mg Se/kg DM either from SS or SY. Concentrations of Se in blood and tissues were found to be significantly increased in both supplemented groups. No response in Se deposition was recorded in the musculus longissimus dorsi of sheep given dietary SS. The intake of SY resulted in a significantly higher Se level in the blood, kidney medulla, skeletal muscles, heart, intestinal and ruminal mucosa than in the case of SS supplementation. No differences appeared between tissue Se contents in the liver and kidney cortex due to the source of added Se. Regardless of source, Se supplementation to feeds significantly increased the glutathione peroxidase (GPx) activity in blood and tissues except the kidney medulla and jejunal mucosa. Supplementation with SY resulted in significantly higher activity of thioredoxin reductase in the liver and ileal mucosa, and also reduced malondialdehyde content in the liver and duodenal mucosa. Dietary Se intake increased Se concentrations in the total rumen contents and bacterial and protozoal fractions. The accumulation of Se in rumen microbiota was associated with increased GPx activity. Phagocytic cell activity was enhanced by Se supplementation. Our results indicate that Se from both sources has beneficial effects on antioxidant status in sheep and can be utilized by rumen microflora.

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Effect of supplementation of various selenium forms and doses on selected parameters of ruminal fluid and blood in sheep

Cited by 29 publications

References 46 publications

Effect of dietary supplementation with selenium-enriched yeast or sodium selenite on ruminal enzyme activities and blood chemistry in sheep

Effect of dietary supplementation with selenium-enriched yeast or sodium selenite on ruminal enzyme activities and blood chemistry in sheep

Copper metabolism in goat-kid relationship at supplementation of inorganic and organic forms of copper

Effects of Different Dietary Selenium Sources on Antioxidant Status and Blood Phagocytic Activity in Sheep

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