Effect of supplementation of Bacillus subtilis LS 1-2 to broiler diets on growth performance, nutrient retention, caecal microbiology and small intestinal morphology

Sen, Sinol; Ingale, S.L.; Kim, Y.W.; Kim, J.S.; Kim, K.H.; Lohakare, J. D.; Kim, E.K.; Kim, H.S.; Ryu, Mun-Ho; Kwon, I.K.; Chae, B. J.

doi:10.1016/j.rvsc.2011.05.021

Cited by 222 publications

(143 citation statements)

References 32 publications

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“…In the 10 ml/L kefir group, a shortening in the intestinal villi or villous atrophy was detected. By contrast, the higher levels of B. subtilis LS 1-2 led to an increase in the villus height in duodenum and ileum [26] . Moreover, the yeast derivatives improved the numbers of intestinal goblet cells, while reducing those of enterocytes undergoing apoptosis in broilers [40] .…”

Section: Yeni̇ce çElebi̇ Yörük Uçar Sağlam Tunç Altunmentioning

confidence: 78%

“…Lactobacillus can be classified as 'colonising' species [1] , but its amount was markedly decreased in kefir-treated layers herein. The potential of probiotics to improve the beneficial bacteria while possibly suppressing the pathogenic ones in the intestines have been shown previously [8,17,26,27] . Further, an increase of beneficial microorganisms in the intestine affected the performance favourably [28] .…”

Section: Yeni̇ce çElebi̇ Yörük Uçar Sağlam Tunç Altunmentioning

confidence: 99%

“…Probiotics affect the host animal beneficially by both improving its intestinal balance and creating gut micro-ecological conditions that suppress harmful microorganisms like Campylobacters, Clostridium, Salmonella and Coliforms [5,15,19,26] , and by favouring the beneficial ones like Lactobacillus and Bifidobacterium. Kefir is claimed to act against the pathogens and to have some antiinflammatory activities [18,19] .…”

Section: Yeni̇ce çElebi̇ Yörük Uçar Sağlam Tunç Altunmentioning

confidence: 99%

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Effect of Kefir upon the Performance, Intestinal Microflora and Histopathology of Certain Organs in Laying Hens

Yenice¹,

Çelebi²,

Yörük³

et al. 2014

Kafkas Univ Vet Fak Derg

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SummaryIn the current study, the effect of kefir upon the performance, intestinal microflora and histopathology of certain organs in laying hens was investigated. Totally, 108 Lohmann Brown layers, aged 24 weeks, were allocated randomly into three groups, as; Group C (control, n=36): no treatment, Group A (n=36): 10 cc, and Group B (n=36): 7.5 cc kefir per litre of water. Animals were fed for 10 weeks with basal diets. Livers showed moderate level of hydropic degeneration, some lipidosis and focal haemorrhages with high amounts of kefir (Group A). Fewer active follicles in the ovarium were also observed in this group. The egg yield was significantly (P<0.01) lower in Group A (89.40±0.91) than in Group C (92.50±0.83) and Group B (92.86±0.87). For the pH of large intestines, unlike the small ones, it was significantly changed (P<0.01) from basic to acidic milieu in kefir-treated groups. The titres of coliform (E. coli), aerobic (Lactobacillus spp.), and anaerobic bacteria (Peptostreptococcus spp.) were significantly decreased (P<0.05 to P<0.001) with increased intake for both intestinal tracts. We conclude that; i) high kefir intake could unfavourably impair the digestive organ structures, ii) the supplementations led to a marked decrease in the large intestinal pH and microbiological load of the intestines, and iii) high kefir level markedly decreased the egg yield, unlike the low concentration as leading to a considerable improvement from the 6 th weeks onwards. Keywords: Kefir, Laying hen, Microbiology, Pathology, Probiotic Yumurtacı Tavuklarda Kefirin Performans, Barsak Mikroflorası ve Bazı Organların Histopatolojisi Üzerine Etkisi ÖzetMevcut araştırmada, yumurtacı tavuklarda kefirin performans, barsak mikroflorası ve bazı organların histopatolojisi üzerine etkisi araştırıldı. 24 haftalık toplam 108 adet Lohmann Brown yumurtacı tavuk; Grup K (kontrol, n=36), Grup A (n=36): 10 cc ve Grup B (n=36): 7.5 cc kefir/L su olarak rastgele 3 gruba ayrıldı. Hayvanlar 10 hafta süreyle bazal rasyonla beslendi. Yüksek kefir miktarı (Grup A), karaciğerlerde orta düzey hidropik dejenerasyon, belli düzeyde lipidosis ve fokal hemorajiler oluşturdu. Ayrıca, bu grupta ovaryumdaki aktif follikül sayısının daha az olduğu gözlendi. Grup A'daki yumurta verimi (89.40±0.91), Grup C (92.50±0.83) ve Grup B'dekinden (92.86±0.87) önemli düzeyde (P<0.01) daha düşük bulundu. Kefir uygulanan gruplardaki kalın bağırsak pH'sı, ince bağırsakların aksine, bazikten asidik ortama doğru önemli düzeyde (P<0.01) değişti. Koliform (E. coli), aerobik (Lactobacillus spp.), ve anaerobik bakteri (Peptostreptococcus spp.) titreleri artan probiyotik alımıyla birlikte önemli düzeyde (P<0.05 -P<0.001) azaldı. Sonuç olarak; i) yüksek kefir alımının sindirim organı yapılarını olumsuz yönde etkileyebildiği, ii) katkıların kalın bağırsak pH'sını ve bağırsakların mikrobiyolojik yükünü önemli düzeyde azalttığı, ve iii) yüksek kefir düzeyinin yumurta verimini önemli düzeyde azaltmasına karşın, düşük konsantrasyonun 6. haftadan sonra belli oranda artırdığı kanısına va...

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Section: Yeni̇ce çElebi̇ Yörük Uçar Sağlam Tunç Altunmentioning

confidence: 78%

Section: Yeni̇ce çElebi̇ Yörük Uçar Sağlam Tunç Altunmentioning

confidence: 99%

Section: Yeni̇ce çElebi̇ Yörük Uçar Sağlam Tunç Altunmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Kefir upon the Performance, Intestinal Microflora and Histopathology of Certain Organs in Laying Hens

Yenice¹,

Çelebi²,

Yörük³

et al. 2014

Kafkas Univ Vet Fak Derg

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“…These findings are in line with the results of Samanya & Yamauchi (2002), who reported that B. subtilis led to an increase in villus height in the small intestine. Abdel-Raheem et al (2012) and Sen et al (2012) reported that dietary supplementations of B. subtilis increased villus length in comparison with the dietary control. In a later study, B. subtilis was found to increase the length and surface area of villi when compared with the positive control group under challenge by C. perfringens (Jayaraman et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Effects of dietary inclusion of probiotics, prebiotics and synbiotics on intestinal histological changes in challenged broiler chickens

Al-Baadani¹,

Abudabos²,

Al-Mufarrej³

et al. 2016

SA J. An. Sci.

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The present study aimed at evaluating the effects of probiotics, prebiotics and their combination on intestinal histological changes in broiler chickens challenged with Clostridium perfringens. Two hundred and forty newly hatched male broilers (Ross 308) were equally distributed into six treatments: negative control group: unsupplemented unchallenged birds; positive control group: unsupplemented challenged birds; neoxyval-fed group: 0.5 g/kg diet (antibiotic) challenged birds; GalliPro-fed group: 0.6 g/kg diet (probiotic), challenged birds; TechnoMos-fed group: 0.75 g/kg diet (prebiotic), challenged birds; and synbiotic-fed group: birds challenged by C. Perfringens (4 x 10 8 CFU /mL). At 35 days old, samples from the ileum and jejunum were collected for histological measurements (height, width and villus surface area), and histopathological changes were assessed. The results showed that the length and surface area of villi in the ileum and jejunum were lower in the positive control, whereas length and surface area of villi in the ileum and jejunum of all feed additive treatments were higher, compared with the positive control and antibiotics. Necrotic enteritis (NE) development was evident in the positive control group. Signs of necrosis of the mucosal membranes, haemorrhage and fibrosis with depletions of gut associated lymph follicle (GALF) in submucosal layer were very clear. Pathological changes improved in all feed additives, except for the antibiotics. These results indicated that alternatives to antibiotics could be used to improve the length and surface area of a villus and the health of the animal by suppressing C. perfringens.

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“…Quando há densa população de micro-organismos ocorrem mudanças na fisiologia intestinal, com aceleração ou atenuação do turnover celular prejudicando a digestão, o transporte e absorção de nutrientes (Leser & Molbak, 2009). O uso de aditivos nutricionais na dieta promove maior densidade dos vilos em frangos de corte (Pelicano 2006, Sen et al 2012). Como exemplo de fatores agressores à mucosa intestinal, aves inoculadas com Salmonella Enteritidis apresentam maiores quantidades de células caliciformes em relação às tratadas com ácidos orgânicos .…”

Section: Introductionunclassified

Influência da qualidade microbiológica da água de dessedentação na morfologia intestinal de frangos de corte

et al. 2015

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Para avaliar se a qualidade microbiológica da água de dessedentação intervém na morfologia intestinal de frangos de corte, foram analisados o consumo semanal de água, a microbiologia de amostras de água, a microscopia eletrônica de varredura e a histologia do intestino delgado de frangos de corte tratados com água filtrada e não filtrada. Os frangos que ingeriram água filtrada tiveram acesso ao menor número de micro-organismos fecais (2,52±0,99 Número Mais Provável (NMP) de coliformes fecais e 1,17±1,25 NMP de Escherichia coli) em relação aos que ingeriram água não filtrada (3,62±0,67 NMP e 2,53±1,13 NMP). Aos 14, 21 e 45 dias de vida, foram colhidas amostras do duodeno, jejuno e íleo de 96 aves. Após rotina laboratorial, as amostras conservadas em glutaraldeído foram eletronmicrografadas e analisadas quanto à densidade de vilos e o material mantido em solução de Bouin foi destinado à confecção de lâminas histológicas que foram analisadas morfometricamente. O duodeno das aves que receberam água não filtrada apresentou maior densidade dos vilos em resposta à qualidade microbiológica da água. Na morfometria intestinal, observou-se que aves que receberam água não filtrada apresentaram aumento na profundidade das criptas intestinais e elevada altura das vilosidades em relação às aves que ingeriram água filtrada. Infere-se que a água filtrada, oferecida aos frangos de corte em um período de vida de 45 dias, favorece a manutenção da integridade intestinal.

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Effect of supplementation of Bacillus subtilis LS 1-2 to broiler diets on growth performance, nutrient retention, caecal microbiology and small intestinal morphology

Cited by 222 publications

References 32 publications

Effect of Kefir upon the Performance, Intestinal Microflora and Histopathology of Certain Organs in Laying Hens

Effect of Kefir upon the Performance, Intestinal Microflora and Histopathology of Certain Organs in Laying Hens

Effects of dietary inclusion of probiotics, prebiotics and synbiotics on intestinal histological changes in challenged broiler chickens

Influência da qualidade microbiológica da água de dessedentação na morfologia intestinal de frangos de corte

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