Effect of Supercritical Carbon Dioxide Extraction Parameters on the Biological Activities and Metabolites Present in Extracts from Arthrospira platensis

Esquivel-Hernández, Diego; Rodríguez‐Rodríguez, José; Cuéllar‐Bermúdez, Sara P.; García‐Pérez, J. Saúl; Mancera-Andrade, Elena I.; Núñez-Echevarría, Jade E.; Ontiveros‐Valencia, Aura; Rostro-Alanís, Magdalena; García‐García, Rebeca; Torres, J. Antonio; Chen, Wei; Parra‐Saldívar, Roberto

doi:10.3390/md15060174

Cited by 28 publications

(22 citation statements)

References 67 publications

(105 reference statements)

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“…In regard to the composition of monosaccharides from depleted cyanobacterial biomass, it has been reported that these microorganisms have different types of polysaccharides, peptoglycanes in the wall cell and glycogen granules in the intracellular vesicles (Van Eykelenburg, 1977). During the SFE process, the cell wall of the cyanobacteria is broken due to the high temperature and pressure required for the process (Esquivel-Hernández et al, 2017a). This leads to the release of the different polysaccharides embedded in the cell wall.…”

Section: Resultsmentioning

confidence: 99%

“…The cyanobacterial strain Arthrospira platensis used throughout this work was cultivated as reported in (Esquivel-Hernández et al, 2017a) (Esquivel-Hernández et al, 2017b.…”

Section: Cyanobacteria Cultivationmentioning

confidence: 99%

“…Supercritical fluid extraction (SFE) of high value metabolites from the A. platensis cells was performed according to our previous work (Esquivel-Hernández et al, 2017a).…”

Section: Extraction Of High Value Metabolites From Cyanobacteria By Smentioning

confidence: 99%

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Integrated biorefinery fromArthrospira platensisbiomass as feedstock for bioethanol and lactic acid production

Esquivel-Hernández

Pennacchio

Parra‐Saldívar

et al. 2019

Preprint

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An integrated biorefinery for ethanol and lactic acid production from the biomass of cyanobacterium Arthrospira platensis was investigated. Different pretreatments consisting of supercritical fluid extraction (SFE) and microwave assisted extraction (MAE) with non-polar (MAE-NPS) and polar solvents (MAE-PS) were tested on cyanobacterial biomass to obtain bioactive metabolites and the resulting residual biomass was used as a substrate for fermentation with Saccharomyces cerevisiae LPB-287 and Lactobacillus acidophilus ATCC 43121 to produce ethanol and lactic acid, respectively. The maximum concentrations achieved in our processes were 3.02±0.07 g/L of ethanol by the MAE-NPS process at 120 rpm 30 ºC, and 9.67±0.05 g/L of lactic acid by the SFE process at 120 rpm 37 ºC. Our results suggest that the proposed approach can be successfully applied in bioactive metabolites extraction and subsequently in the production of Ethanol and Lactic acid from A. platensis depleted biomass.

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Section: Resultsmentioning

confidence: 99%

“…The cyanobacterial strain Arthrospira platensis used throughout this work was cultivated as reported in (Esquivel-Hernández et al, 2017a) (Esquivel-Hernández et al, 2017b.…”

Section: Cyanobacteria Cultivationmentioning

confidence: 99%

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Integrated biorefinery fromArthrospira platensisbiomass as feedstock for bioethanol and lactic acid production

Esquivel-Hernández

Pennacchio

Parra‐Saldívar

et al. 2019

Preprint

Self Cite

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“…14 Pressure and temperature have to be chosen appropriately to exceed the supercritical point of CO2 at 304.12 K and 73.7 bar. 14,15 Subsequently, they have to be kept high enough, to preserve the CO2 in supercritical state. For choosing temperature and pressure, it has to be taken into account that increasing percentage of co-solvent increases pressure and temperature required to reach the supercritical point of the mixture.…”

Section: Introductionmentioning

confidence: 99%

Supercritical Fluid Extraction Enhances Discovery of Secondary Metabolites from Myxobacteria

Bader

Neuber²,

Panter³

et al. 2020

Preprint

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Supercritical fluid extraction (SFE) is widely used for the isolation of natural products from plants, but its application in efforts to identify structurally and physicochemically often dissimilar microbial natural products is limited to date. In this study we evaluated the impact of SFE on the extractability of myxobacterial secondary metabolites aiming to improve the prospects of discovering novel natural products. We investigated the influence of different co-solvents on the extraction efficiency of secondary metabolites from three myxobacterial strains as well as the antimicrobial activity profiles of the corresponding extracts. For each known secondary metabolite we found extraction conditions using SFE leading to superior yields in the extracts compared to conventional solvent extraction. Compounds with a logP higher than 3 showed best extraction efficiency using 20% EtOAc as a co-solvent, whereas compounds with logP values lower than 3 were better extractable using more polar co-solvents like MeOH. Extracts generated with SFE showed increased antimicrobial activities including the presence of activities not explained by known myxobacterial secondary metabolites, highlighting the advantage of SFE for bioactivity-guided isolation. Moreover, non-targeted metabolomics analysis revealed a group of chlorinated metabolites produced by the well-studied model myxobacterium Myxococcus xanthus DK1622 which were not accessible previously due to their low concentration in conventional extracts. The enriched SF extracts were used for isolation and subsequent structure elucidation of chloroxanthic acid A as founding member of a novel secondary metabolite family. Our findings encourage the increased utilization of SFE as part of future microbial natural products screening workflows.

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“…The literature proposes different lutein extraction technologies from S. almeriensis but the findings are not sufficient to establish a clear methodological and a defined approach for the evaluation of extraction costs, yield, and quality of the final extracts [19,20,21,22,23,24,25,26].…”

Section: Introductionmentioning

confidence: 99%

Supercritical Fluid Extraction of Lutein from Scenedesmus almeriensis

et al. 2019

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Lutein has several benefits for human health, playing an important role in the prevention of age-related macular degeneration (AMD), cataracts, amelioration of the first stages of atherosclerosis, and some types of cancer. In this work, the Scenedesmus almeriensis microalga was used as a natural source for the supercritical fluid (SF) extraction of lutein. For this purpose, the optimization of the main parameters affecting the extraction, such as biomass pre-treatment, temperature, pressure, and carbon dioxide (CO2) flow rate, was performed. In the first stage, the effect of mechanical pre-treatment (diatomaceous earth (DE) and biomass mixing in the range 0.25–1 DE/biomass; grinding speed varying between 0 and 600 rpm, and pre-treatment time changing from 2.5 to 10 min), was evaluated on lutein extraction efficiency. In the second stage, the influence of SF-CO2 extraction parameters such as pressure (25–55 MPa), temperature (50 and 65 °C), and CO2 flow rate (7.24 and 14.48 g/min) on lutein recovery and purity was investigated. The results demonstrated that by increasing temperature, pressure, and CO2 flow rate lutein recovery and purity were improved. The maximum lutein recovery (~98%) with purity of ~34% was achieved operating at 65 °C and 55 MPa with a CO2 flow rate of 14.48 g/min. Therefore, optimum conditions could be useful in food industries for lutein supplementation in food products.

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Effect of Supercritical Carbon Dioxide Extraction Parameters on the Biological Activities and Metabolites Present in Extracts from Arthrospira platensis

Cited by 28 publications

References 67 publications

Integrated biorefinery fromArthrospira platensisbiomass as feedstock for bioethanol and lactic acid production

Integrated biorefinery fromArthrospira platensisbiomass as feedstock for bioethanol and lactic acid production

Supercritical Fluid Extraction Enhances Discovery of Secondary Metabolites from Myxobacteria

Supercritical Fluid Extraction of Lutein from Scenedesmus almeriensis

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