Effect of Suo Quan Wan on the bladder function of aging rats based on the β-adrenoceptor

Xu, Yifei; Liang, Zhijian; Kuang, Zhao‐Jin; Chen, Jiejun; Wu, Jun; Lu, Xiange; Jiang, Weiwen; Fan, Pinglong; Tang, Liyao; Li, Yi‐Tao; Huang, Ping; Cao, Hongying

doi:10.3892/etm.2017.4394

Cited by 12 publications

(14 citation statements)

References 28 publications

(21 reference statements)

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“…Aging is mainly characterized by a progressive dysfunction of metabolism and various physiological roles, such as maintaining metabolic rate and delaying cell aging ( 1 , 2 ). Clinically, abnormalities of the metabolism and physiological functions can affect the morbidity and mortality of patients.…”

Section: Introductionmentioning

confidence: 99%

Ginsenoside Rg1 protects against Sca‑1+ HSC/HPC cell aging by regulating the SIRT1‑FOXO3 and SIRT3‑SOD2 signaling pathways in a γ‑ray irradiation‑induced aging mice model

et al. 2020

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Aging is characterized by a progressive deterioration in metabolic functions. The present study aimed to investigate the antagonistic effects of ginsenoside Rg1 (Rg1) on the γ-ray irradiation-induced aging of mixed hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs). C57BL/6 mice were divided into a control group, a γ-ray irradiation group that served as an aging mouse model, and an Rg1 group. The Rg1 group was treated with Rg1 at dosage of 20 mg/kg/day for 7 days prior to γ-ray irradiation. The aging mouse model was established by exposing the mice to 6.5-Gy γ-ray total-body irradiation. Stem cell antigen 1 positive (Sca-1 + ) HSC/HPCs isolated from the mice were examined using a senescence-associated β-galactosidase (SA-β-Gal) staining assay. The cell cycle of the HSC/HPCs was examined using flow cytometry. A mixed hematopoietic progenitor cell colony-forming unit (CFU-mix) assay was also conducted. The mRNA and protein expression levels of sirtuin 1 (SIRT1), SIRT3, forkhead box O3 (FOXO3) and superoxide dismutase (SOD2) were evaluated using western blot and reverse transcription-quantitative PCR assays. The results indicated that Rg1 treatment significantly increased white blood cell, red blood cell and platelet counts in peripheral blood compared with those in the γ-ray irradiation group (P<0.05). However, Rg1 significantly attenuated the senescence of Sca-1 + HSC/HPCs in the γ-ray irradiation aging mice model. The proportion of SA-β-Gal stained HSC/HPCs was significantly decreased and CFU-Mix counts were significantly increased in the Rg1 group compared with the γ-ray irradiation group (P<0.05). Rg1 significantly increased the mRNA and protein levels of SIRT1, SIRT3, FOXO3 and SOD2 in the Sca-1 + HSC/HPCs compared with those in the γ-ray irradiation group (P<0.05). The percentage of Sca-1 + HSC/HPCs arrested at the G1 phase in the Rg1 group was significantly decreased compared with that in the γ-ray irradiation group (P<0.05). In conclusion, the present study indicates that Rg1 exerts anti-aging effects via the regulation of SIRT1-FOXO3 and SIRT3-SOD2 signaling pathways, and triggering the progression of Sca-1 + HSC/HPCs from the G1 phase to the S phase in γ-ray irradiation-induced aging mice.

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Section: Introductionmentioning

confidence: 99%

Ginsenoside Rg1 protects against Sca‑1+ HSC/HPC cell aging by regulating the SIRT1‑FOXO3 and SIRT3‑SOD2 signaling pathways in a γ‑ray irradiation‑induced aging mice model

et al. 2020

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“…In recent years, the use of traditional Chinese medicine and natural products in the treatment of diabetic and its complications has become noteworthy due to its effectiveness and less toxicity [11]. Radix Linderae (RL; named Wu-Yao in Chinese), the dried root of Lindera aggregata (Sims) Kosterm, was the main component of Suo Quan Wan, which has been shown to enhance bladder storage and contraction ability [12] and improve bladder voiding function based on the regulation of transient receptor potential vanilloid-1 (TRPV1) [13, 14]. Furthermore, RL has been used for centuries to combat frequent urination and other urinary bladder diseases [15–17].…”

Section: Introductionmentioning

confidence: 99%

Effects of Radix Linderae extracts on a mouse model of diabetic bladder dysfunction in later decompensated phase

Yuan

Lian

Fan

et al. 2019

BMC Complement Altern Med

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BackgroundThis study aimed to elucidate the effects and mechanisms of Radix Linderae (RL) extracts on a mouse model of diabetic bladder dysfunction (DBD), especially on later decompensated phase.MethodsMale C57BL/6J mice were intraperitoneally injected with streptozotocin (STZ) after 4 weeks of high-fat diet (HFD) feeding. DBD mouse models (later decompensated phase) were developed by 12-weeks persistent hyperglycemia and then treated with RL extracts for 4 weeks. During administration, the fasting blood glucose (FBG) test was performed once a week. Four weeks later, oral glucose tolerance test (OGTT), voided stain on paper (VSOP), and urodynamic alteration were explored. We also performed haematoxylin and eosin (H&E) and Masson’s trichrome staining to observe the histology of the bladder. Then, the contractile responses to α, β-methylene ATP, capsaicin (CAP), KCl and carbachol were measured. Moreover, qPCR assay was performed to analyse the bladder gene expression levels of M3 receptors and TRPV1.ResultsThe diabetic mice exhibited higher FBG, OGTT and urine production, and no substantial alteration was observed after RL treatment. Urodynamic test showed the maximum bladder capacity (MBC), residual volume (RV) and bladder compliance (BC), as well as the decrement of voided efficiency (VE) and micturition volume (MV), remarkably increased in the DBD mice. Furthermore, RL treatment significant improved urodynamic urination, with lower MBC, RV, and, BC, as well as higher VE and MV, as compared with the model groups. The wall thickness of the bladder and the ratio of smooth muscle/collagen remarkably increased, and RL could effectively attenuate the pathological change. The response of bladder strips to the stimulus was also reduced in the DBD mice, and RL treatment markedly increased the contraction. Furthermore, the gene expression levels of M3 receptors and TRPV1 were down-regulated in the bladders of the diabetic mice, whereas RL treatment retrieved those gene expression levels.ConclusionsRL extracts can improve the bladder voiding functions of the DBD model mice in later decompensated phase, and underlying mechanisms was associated with mediating the gene expression of M3 receptors and TRPV1 in the bladder instead of improving blood sugar levels.Electronic supplementary materialThe online version of this article (10.1186/s12906-019-2448-1) contains supplementary material, which is available to authorized users.

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“…[ 22 ] Suoquan is a mixture of Alpinia oxyphylla Miq., Dioscorea opposita Thunb., and Radix Lindera, prepared at a ratio of 1:1:1, and is used to warm kidney yang and expel cold while relieving frequent urination by stopping leakage. [ 23 – 25 ] In South Korea, Suoquan is widely used for ameliorating urinary tract symptoms, such as NE and frequent or urgent urination. [ 26 ] Various forms of Suoquan are available, such as pills, capsules, tablets, and decoctions.…”

Section: Discussionmentioning

confidence: 99%

Herbal medicine (Suoquan) for treating nocturnal enuresis

Lee

Jang

et al. 2018

Medicine

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Effect of Suo Quan Wan on the bladder function of aging rats based on the β-adrenoceptor

Cited by 12 publications

References 28 publications

Ginsenoside Rg1 protects against Sca‑1+ HSC/HPC cell aging by regulating the SIRT1‑FOXO3 and SIRT3‑SOD2 signaling pathways in a γ‑ray irradiation‑induced aging mice model

Ginsenoside Rg1 protects against Sca‑1+ HSC/HPC cell aging by regulating the SIRT1‑FOXO3 and SIRT3‑SOD2 signaling pathways in a γ‑ray irradiation‑induced aging mice model

Effects of Radix Linderae extracts on a mouse model of diabetic bladder dysfunction in later decompensated phase

Herbal medicine (Suoquan) for treating nocturnal enuresis

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